本文選題：奶牛臨床乳房炎　切入點(diǎn)：大腸桿菌　出處：《寧夏大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：為了研究寧夏地區(qū)奶牛臨床乳房炎大腸桿菌分離株血清型分布和主要毒力基因,采集2013-2014年寧夏地區(qū)臨床乳房炎病牛奶樣作為大腸桿菌檢測(cè)的樣品,進(jìn)行分離培養(yǎng),采用細(xì)菌生化試驗(yàn)進(jìn)行鑒定。使用大腸桿菌0抗原診斷血清對(duì)分離自奶牛臨床乳房炎的120株大腸桿菌分離株進(jìn)行血清學(xué)檢測(cè),并利用PCR方法擴(kuò)增定居因子基因fimH、f17、f165、f41、 f5、eae、cs31a和轉(zhuǎn)運(yùn)蛋白trat基因。采集2013-2014年寧夏地區(qū)臨床乳房炎病牛奶樣作為大腸桿菌檢測(cè)的樣品,進(jìn)行分離培養(yǎng),采用細(xì)菌生化試驗(yàn)進(jìn)行行鑒定,120株鑒定為大腸桿菌。大腸桿菌O抗原鑒定結(jié)果：經(jīng)鑒定120株奶牛臨床乳房炎大腸桿菌分離株中有71株檢測(cè)出血清型,鑒定出08,010,015,018,022,O 26,053,055,068,086,091,092,093,0101,0107,0117,0126,0142,0148,0153,0158共21種血清型。優(yōu)勢(shì)血清型為0158,0101,0126,091,015,026,0107,占定型株的61.9%,49株未鑒定出血清型。選擇trat和7種定居因子fimH、f17、f165、f41、f5、eae、cs31a進(jìn)行檢測(cè)。實(shí)驗(yàn)結(jié)果顯示,120株大腸桿菌PCR擴(kuò)增結(jié)果為trat基因分離率為24.2%,fimH基因分離率為85.8%,f17基因分離率為5%,f165基因分離率為3.3%,f41基因分離率為0,f5基因分離率,0,eae基因分離率為0,cs31a基因分離率0。實(shí)驗(yàn)結(jié)果表明腸道內(nèi)感染和臨床乳房炎大腸桿菌分離株血清型、毒力基因分離率不同。本實(shí)驗(yàn)選取奶牛臨床乳房炎大腸桿菌分離株(0158)為樣品,設(shè)計(jì)并合成引物,對(duì)fimH基因進(jìn)行克隆和序列測(cè)定.測(cè)序結(jié)果顯示fimh基因在第714堿基位點(diǎn)位點(diǎn)由T突變?yōu)锳,在第717個(gè)堿基位點(diǎn)位點(diǎn)由A突變?yōu)镚,第807個(gè)堿基位點(diǎn)位點(diǎn)由G突變?yōu)锳,第831個(gè)堿基位點(diǎn)位點(diǎn)由T突變?yōu)镃。
[Abstract]:In order to study the distribution of serotype and the main virulence genes of clinical mastitis Escherichia coli isolated from dairy cattle in Ningxia, samples of clinical mastitis in Ningxia from 2013 to 2014 were collected as samples for detection of Escherichia coli, and were isolated and cultured. The serological tests of 120 strains of Escherichia coli isolated from clinical mastitis of dairy cattle were carried out by using the diagnostic serum of Escherichia coli 0 antigen. The PCR method was used to amplify the gene fimHf17f165f41, f5eaeae cs31a and transporter trat. The samples of clinical mastitis in Ningxia from 2013 to 2014 were collected as samples for detection of Escherichia coli, and were isolated and cultured. A total of 120 strains of Escherichia coli were identified by bacterial biochemistry test. Results of the identification of Escherichia coli O antigen: 71 of 120 strains of clinical mastitis Escherichia coli isolated from dairy cattle were identified as serotype. 21 serotypes were identified from 080100 150 18022 O260530550680880860910920310101n010701177, 012614801480153150158. The dominant serotype was 0158O1010101260910260107, which accounted for 61.9107.The trat and the seven settlement factor fimHf17f165f41f41a were selected for detection. The results showed that the PCR amplification of 120 strains was trat based Escherichia coli. Because the isolation rate of fimH gene was 24.2and the isolation rate of f17 gene was 85.8.The isolation rate of 5f165 gene was 3.3f41 gene and the isolation rate of f41 gene was zero f5 gene. The isolation rate of 0eae gene was 0 cs31a gene. The results showed that intestinal tract infection and clinical mastitis were caused by intestinal infection and clinical mastitis. Serotype of Escherichia coli isolates, The isolation rate of virulence gene was different. In this experiment, primers were designed and synthesized by using clinical mastitis Escherichia coli strain 0158 as the sample. FimH gene was cloned and sequenced. The results of sequencing showed that fimh gene mutated from T to A at 714 base site, from A to G at 717 base site, from G to A at 807 base site, and from G to A at 714 base site. 831 base loci were mutated from T to C.
【學(xué)位授予單位】：寧夏大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S852.61

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 劉廣亮;;奶牛的干奶策略及乳房炎防制[J];養(yǎng)殖技術(shù)顧問;2009年11期

2 胡菊梅;溫嘉琪;鄭曉培;王娟;;奶牛乳房炎病原分離鑒定及其治療方法研究[J];畜牧獸醫(yī)雜志;2012年01期

3 劉蘇萌;鄒方東;;體細(xì)胞計(jì)數(shù)及其在降低奶牛乳房炎發(fā)病率方面的應(yīng)用[J];中國畜牧雜志;2006年09期

4 斯木吉德;敖日格樂;王純潔;吳美云;;奶牛新鮮糞便致病性大腸桿菌分離鑒定[J];中國獸醫(yī)雜志;2009年08期

5 喻華英;周麗婷;;致犢牛腹瀉埃希氏菌eaeA基因克隆和序列分析[J];西北農(nóng)業(yè)學(xué)報(bào);2013年04期

，

本文編號(hào)：1644970