本文選題：白藜蘆醇　切入點(diǎn)：豬睪丸間質(zhì)細(xì)胞　出處：《湖南農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：白藜蘆醇作為一種天然產(chǎn)物的提取物,研究發(fā)現(xiàn)它不但在抗腫瘤、抗心血管疾病、抗炎、抗菌抗病毒、抗衰老方面有影響,而且在生殖系統(tǒng)中也起著很重要的作用。為探究其抗氧化損傷作用,本論文通過體外細(xì)胞實(shí)驗(yàn),并結(jié)合體內(nèi)動(dòng)物實(shí)驗(yàn)研究了白藜蘆醇的抗氧化作用。體外細(xì)胞實(shí)驗(yàn)：通過建立豬原代睪丸間質(zhì)細(xì)胞體系,用誘導(dǎo)劑過氧化氫建立了豬原代睪丸間質(zhì)細(xì)胞的氧化損傷模型,一定劑量的(150 μM或以上)過氧化氫處理間質(zhì)細(xì)胞能影響其形態(tài)學(xué)變化,抑制細(xì)胞的增殖,細(xì)胞發(fā)生凋亡的比例明顯增加,相應(yīng)的抗氧化成分超氧化物歧化酶SOD顯著降低,而且細(xì)胞分泌睪酮能力顯著下降；低劑量的白藜蘆醇處理睪丸間質(zhì)細(xì)胞可以促進(jìn)細(xì)胞的增殖,濃度為0.2 gM的白藜蘆醇對(duì)細(xì)胞作用效果較好；另外,用白藜蘆醇可以在一定程度上修復(fù)由過氧化氫所引起的細(xì)胞氧化損傷,表現(xiàn)為：減少過氧化氫對(duì)細(xì)胞的抑制；提高氧化損傷細(xì)胞中抗氧化物質(zhì)SOD的產(chǎn)生；同時(shí)提高氧化損傷細(xì)胞分泌睪酮的能力并減少細(xì)胞的凋亡。體外白藜蘆醇的抗氧化損傷作用可為后續(xù)的體內(nèi)動(dòng)物實(shí)驗(yàn)提供基礎(chǔ)。體內(nèi)動(dòng)物實(shí)驗(yàn)：選用未成年雄性小鼠為實(shí)驗(yàn)動(dòng)物,用環(huán)磷酰胺建立小鼠生精障礙模型,生殖毒性作用比較明顯,現(xiàn)為小鼠的睪丸指數(shù)和附睪指數(shù)顯著下降；總精子數(shù)下降；精子畸形率升高；睪丸出現(xiàn)較明顯的病理形態(tài)學(xué)變化；睪酮分泌下降；還影響小鼠的生長(zhǎng)；當(dāng)用白藜蘆醇處理環(huán)磷酰胺造模的小鼠時(shí),能在一定程度上減少其生殖毒性,表現(xiàn)為：體重增加、睪丸指數(shù)、附睪指數(shù)和精子總數(shù)顯著增加,相應(yīng)的精子畸形率減少、睪丸病理形態(tài)學(xué)變化減輕以及血清中睪酮含量增加,但血清中抗氧化成分SOD和谷胱甘肽酶GSH-Px沒有顯著變化；另外,單獨(dú)用40 mg/kg · d劑量的白藜蘆醇處理未成年雄性小鼠對(duì)其生殖不造成影響。以上結(jié)果表明：白藜蘆醇對(duì)氧化損傷所致的生殖毒性有一定的治療作用,但其具體作用機(jī)制還需進(jìn)一步研究。相關(guān)結(jié)果白藜蘆醇作為一種體內(nèi)外抗氧化藥物或作為一種飼料添加劑在畜牧生產(chǎn)中的應(yīng)用提供參考。
[Abstract]:Resveratrol as a natural product of extract, research found that it not only in anti-tumor, anti-cardiovascular disease, anti-inflammatory, anti-viral, anti-aging effects, It also plays an important role in the reproductive system. The antioxidation effect of resveratrol was studied in vivo. In vitro cell experiment: the model of oxidative damage of primary testicular interstitial cells of pig was established by using hydrogen peroxide as an inducer. The interstitial cells treated with hydrogen peroxide at a certain dose of 150 渭 M or more could affect the morphological changes, inhibit the proliferation of the cells, increase the proportion of cell apoptosis, and decrease the SOD of superoxide dismutase (SOD). In addition, low dose resveratrol could promote the proliferation of testicular interstitial cells, and the concentration of 0.2 g / g resveratrol had a better effect on the cells. Resveratrol can repair the oxidative damage induced by hydrogen peroxide to some extent, such as reducing the inhibition of hydrogen peroxide on cells, increasing the production of antioxidant SOD in oxidative damage cells. The antioxidative effects of resveratrol in vitro may provide the basis for further in vivo animal experiments. In vivo animal experiments: juvenile males are selected. Mice are experimental animals, Cyclophosphamide was used to establish mouse model of spermatogenesis. The reproductive toxicity was obvious. The testicular index and epididymal index of mice were significantly decreased, the total sperm number was decreased, the sperm deformity rate was increased, the testis had obvious pathomorphological changes. The decrease in testosterone secretion also affected the growth of mice, and when resveratrol was treated with cyclophosphamide, the reproductive toxicity of mice was reduced to a certain extent, as follows: weight gain, testicular index, The epididymal index and total sperm count increased significantly, the corresponding sperm deformity rate decreased, the testicular pathomorphology decreased and the testosterone content in serum increased, but the antioxidant components SOD and glutathione GSH-Px did not change significantly. 40 mg/kg 路d of resveratrol alone had no effect on the reproduction of juvenile male mice. The results showed that resveratrol had a certain therapeutic effect on the reproductive toxicity induced by oxidative injury. Results resveratrol as an antioxidation drug in vivo and in vitro or as a feed additive in animal production provides reference for the application of resveratrol in animal husbandry.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S859.7

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