發(fā)布時間：2018-03-19 10:31

  本文選題：地衣芽胞桿菌　切入點：聚γ谷氨酸　出處：《動物醫(yī)學進展》2017年04期 　論文類型：期刊論文

【摘要】：為探討調節(jié)因子DegR對聚γ谷氨酸(γ-PGA)生物合成產量的影響,比較聚γ谷氨酸佐劑和傳統(tǒng)鋁佐劑的輔助免疫效果,利用P43啟動子構建了穿梭表達載體pHY-degR,通過電轉化地衣芽胞桿菌WX-02獲得重組菌株WX-02/pHYdegR。同時以福爾馬林滅活的金黃色葡萄球菌蛋白疫苗作為免疫原,分別以不同分子質量聚γ谷氨酸佐劑和鋁佐劑制備相關疫苗并免疫小鼠,7d后注射金黃色葡萄球菌,根據(jù)小鼠死亡數(shù),計算其相對免疫保護率。結果顯示,過表達degR后,聚γ谷氨酸生物合成產量提高至28.5g/L,相比對照菌株WX-02/pHY300提高22.6%。分子質量2×10~3 ku聚γ谷氨酸作為佐劑的相對免疫保護率效果最好(78.9%),優(yōu)于鋁佐劑免疫保護率(52.6%)和未添加佐劑免疫保護率(42.1%)。結果表明,過表達degR基因能提高聚γ谷氨酸生物合成量,且聚γ谷氨酸作為疫苗佐劑能增強疫苗免疫效果,可作為理想的疫苗佐劑。
[Abstract]:In order to investigate the effect of DegR on the biosynthesis yield of poly 緯 -glutamic acid (緯 -PGA), the adjuvant effects of 緯 -glutamic acid adjuvant and traditional aluminum adjuvant were compared. The shuttle expression vector pHY-degRwas constructed by using P43 promoter, and the recombinant strain WX-02 / pHYdegRwas obtained by electrotransformation of Bacillus licheniformis WX-02. The protein vaccine of Staphylococcus aureus inactivated by formalin was also used as immunogen. The related vaccines were prepared with different molecular weight poly (緯 -glutamic acid) adjuvant and aluminum adjuvant. Staphylococcus aureus was injected into mice for 7 days after immunization. The relative immune protection rate was calculated according to the number of mice killed. The results showed that, after overexpression of degR, the relative immune protection rate was calculated. The yield of polyglutamic acid biosynthesis was increased to 28.5g / L, which was 22.6g / L higher than that of the control strain WX-02/pHY300. The molecular weight of 2 脳 10 ~ 3 ku poly (緯 -glutamic acid) as adjuvant had the best relative immune protection rate of 78.9%, which was better than that of aluminum adjuvant (52.6%) and no adjuvant was added. The immune protection rate was 42.1%. The results showed that. Overexpression of degR gene can improve the biosynthesis of poly (緯 -glutamate), and polyglutamic acid as a vaccine adjuvant can enhance the immune effect of the vaccine and can be used as an ideal vaccine adjuvant.
【作者單位】： 長江大學動物科學學院;長江大學城市建設學院;
【基金】：湖北省教育廳青年人才基金項目(q20151306) 濕地生態(tài)與農業(yè)利用教育部工程研究中心基金項目(KF201509)
【分類號】：S852.4
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本文編號：1633883