發(fā)布時間：2018-03-18 09:02

  本文選題：膠紅酵母　切入點：固態(tài)發(fā)酵　出處：《西南大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本試驗主要通過對發(fā)酵底物、發(fā)酵條件的優(yōu)化來提高膠紅酵母(Rhodotorula muciladinosa TZR2014)生產(chǎn)類胡蘿卜素的效率,同時研究膠紅膠母發(fā)酵產(chǎn)物(RMFP)對蛋雞生產(chǎn)性能、蛋品質(zhì)、腸道消化酶、盲腸微生物區(qū)系以及血漿和肝臟生化指標(biāo)的影響。發(fā)酵工藝優(yōu)化。首先采用Design-Expert軟件進(jìn)行發(fā)酵底物的優(yōu)化設(shè)計,然后根據(jù)類胡蘿卜素的產(chǎn)量確定最優(yōu)發(fā)酵底物配比;在此基礎(chǔ)上利用正交設(shè)計對接種量、含水量、發(fā)酵溫度、pH和發(fā)酵時間進(jìn)行優(yōu)化設(shè)計,并根據(jù)試驗結(jié)果得到膠紅酵母產(chǎn)類胡蘿卜素的最優(yōu)發(fā)酵條件。研究得出膠紅酵母固態(tài)發(fā)酵底物的最佳配比為麥麩52.5%、豆粕20.0%、玉米粉3.00%、米糠14.0%、玉米漿10.0%、硫酸銨0.40%、磷酸二氫鉀0.05%和硫酸鎂0.04%。最佳發(fā)酵條件為菌液接種量為5.0%、發(fā)酵時間為72.0 h、發(fā)酵溫度為28.0℃、pH為6.0、含水量為60.0%。經(jīng)過發(fā)酵工藝的優(yōu)化,類胡蘿卜素含量提高到4535μg/kg,且發(fā)酵后粗蛋白質(zhì)提高了9.67%。動物試驗。選用體況良好、體重相近的40周齡羅曼蛋雞216只,按隨機(jī)區(qū)組分為4個處理,每組3個重復(fù),每個重復(fù)18只。四組日糧組成分別為87.5%基礎(chǔ)日糧+12.5%發(fā)酵底物(CON)、87.5%基礎(chǔ)日糧+12.0%發(fā)酵底物+0.50%RMFP(0.50%RMFP)、87.5%基礎(chǔ)日糧+10.0%發(fā)酵底物+2.50%RMFP(2.50%RMFP)、87.5%基礎(chǔ)日糧+12.5%RMFP(12.5%RMFP)。試驗期共31 d,其中預(yù)試期為3 d。結(jié)果顯示,日糧添加RMFP可顯著提高蛋雞的采食量和產(chǎn)蛋重(P0.05)、蛋黃類胡蘿卜素含量(P0.05)。日糧添加RMFP可顯著提高蛋雞血漿免疫球蛋白A(IgA)、免疫球蛋白G(IgG)和球蛋白的含量(P0.05);這一營養(yǎng)調(diào)控措施顯著降低蛋雞血漿丙二醛(MDA)含量并提高蛋雞血漿和肝臟的總抗氧化能力(T-AOC)和過氧化氫酶(CAT)活力(P0.05)。日糧添加RMFP可顯著提高十二指腸和空腸食糜糜蛋白酶的活性(P0.05)。日糧添加RMFP可增加蛋雞盲腸內(nèi)有益菌的數(shù)量(如甲烷粒菌屬、熱纖梭菌屬和瘤胃球菌屬),并減少有害菌(如螺桿菌屬)。綜上所述,通過發(fā)酵工藝優(yōu)化可顯著提高類胡蘿卜素的產(chǎn)量。飼喂12.5%的RMFP可顯著提高蛋雞的生產(chǎn)性能,并在一定程度上改善蛋品質(zhì),對蛋雞的抗氧化能力、免疫功能以及盲腸微生物區(qū)系也具有正調(diào)控作用。
[Abstract]:The production efficiency of carotenoid from Rhodotorula muciladinosa TZR2014) was improved by optimizing the fermentation substrate and fermentation conditions. Meanwhile, the production performance, egg quality and digestive enzyme of intestinal tract of laying hens were studied. Effects of microflora of cecum and biochemical indexes of plasma and liver. Optimization of fermentation process. Design-Expert software was used to optimize the fermentation substrate, and then the optimal fermentation substrate ratio was determined according to the yield of carotenoid. On this basis, the orthogonal design was used to optimize the inoculation amount, water content, fermentation temperature, pH and fermentation time. The optimum fermentation conditions for carotenoid production by red gum yeast were obtained. The optimum proportion of solid fermentation substrate was obtained as follows: wheat bran 52.5, soybean meal 20.0, corn flour 3.00, rice bran 14.0, corn pulp 10.0, ammonium sulfate 0.40, phosphoric acid. Potassium dihydrogen 0.05% and magnesium sulfate 0.04. The optimum fermentation conditions were: inoculation amount of bacteria liquid was 5.0, fermentation time was 72.0 hours, fermentation temperature was 28.0 鈩，

本文編號：1628884