本文選題：中藥復(fù)方宮炎凈　切入點(diǎn)：簡(jiǎn)化復(fù)方　出處：《石河子大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過(guò)中獸醫(yī)實(shí)驗(yàn)室前期研究發(fā)現(xiàn),中藥復(fù)方“宮炎凈”主要有效成分為生物堿,本試驗(yàn)擬通過(guò)比較中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方、君藥益母草中的生物堿對(duì)奶牛子宮內(nèi)膜炎主要致病菌金黃色葡萄球菌、大腸桿菌及無(wú)乳鏈球菌的抑菌效果,以及對(duì)小鼠耳廓腫脹、足趾腫脹、腹腔毛細(xì)血管通透性以及肉芽腫炎癥模型的抗炎效果,為優(yōu)化中藥復(fù)方“宮炎凈”提供理論依據(jù)。方法:1.將粗提的自擬中藥復(fù)方“宮炎凈”各單味藥(益母草、川芎、當(dāng)歸、桃仁、甘草、五靈脂、干姜、蒲黃)中加入碘化汞鉀試劑、碘化鉍鉀試劑、硅鎢酸試劑、磷鉬酸試劑,檢測(cè)每味藥中是否含有生物堿,對(duì)初步篩選含有生物堿的各單味藥進(jìn)行精制,將含有生物堿的單味藥按原方劑量配制為簡(jiǎn)化復(fù)方。2.通過(guò)乙醇回流提取法與超聲波輔助提取法分別提取中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方、君藥益母草中的生物堿。3.通過(guò)平板打孔法和試管二倍稀釋法測(cè)定中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方、君藥益母草中的生物堿對(duì)奶牛子宮內(nèi)膜炎主要致病菌金黃色葡萄球菌、無(wú)乳鏈球菌、大腸桿菌體外抑菌效果。4.通過(guò)建立小鼠耳廓腫脹、足趾腫脹、腹腔毛細(xì)血管通透性以及肉芽腫炎癥模型來(lái)評(píng)估中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方、君藥益母草中生物堿的抗炎效果。結(jié)果:1.自擬中藥復(fù)方“宮炎凈”8味藥粗提液分別加入生物堿試劑檢測(cè)發(fā)現(xiàn),益母草、川芎、甘草中可能含有生物堿,通過(guò)進(jìn)一步精制后確定上述三味藥含有生物堿,將益母草、川芎、甘草按照原方劑量配制為簡(jiǎn)化復(fù)方。2.通過(guò)對(duì)比乙醇回流提取法與超聲輔助提取法發(fā)現(xiàn),自擬中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方、君藥益母草中的生物堿,乙醇回流提取法提取率分別為0.92%、0.93%、0.71%,超聲輔助提取法提取率分別為0.87%、0.86%、0.62%。3.通過(guò)體外抑菌試驗(yàn),發(fā)現(xiàn)自擬中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方、益母草中的生物堿對(duì)金黃色葡萄球菌、大腸桿菌及無(wú)乳鏈球菌的平均抑菌圈直徑分別為17.4±0.8mm、16.9±1.2mm、7.8±0.8mm,上述三組生物堿對(duì)金黃色葡萄球菌MIC、MBC分別為32 g/L、125 g/L、500g/L,62.5 g/L、125 g/L、500g/L;對(duì)大腸桿菌MIC、MBC分別為62.5 g/L、125 g/L、250g/L,125 g/L、250 g/L、500g/L;對(duì)無(wú)乳鏈球菌MIC、MBC分別為125 g/L、125 g/L、500g/L,250 g/L、250g/L、未測(cè)出。4.對(duì)小鼠的抗炎試驗(yàn)發(fā)現(xiàn)中藥復(fù)方“宮炎凈”、簡(jiǎn)化復(fù)方及益母草中的生物堿均對(duì)小鼠耳廓腫脹、足部腫脹、腹腔毛細(xì)血管通透性和棉球肉芽腫的形成有抑制作用,其中復(fù)方“宮炎凈”和簡(jiǎn)化復(fù)方的抗炎效果與益母草生物堿組差異顯著(P0.05),與地塞米松組差異不顯著(P0.05),簡(jiǎn)化復(fù)方的抗炎效果略優(yōu)于復(fù)方“宮炎凈”。結(jié)論:簡(jiǎn)化復(fù)方與自擬中藥復(fù)方“宮炎凈”的體外抑菌作用、體內(nèi)抗炎活性均相差不大,為簡(jiǎn)化復(fù)方的臨床運(yùn)用提供了理論依據(jù)。
[Abstract]:Objective: to find out that the main active component of the compound "Gong Yan Jing" is alkaloid through the preliminary study of Chinese Veterinary Laboratory. This experiment aims to simplify the compound by comparing the traditional Chinese medicine compound "Gong Yan Jing". The inhibitory effect of alkaloids in Herba Leonurus on Staphylococcus aureus, Escherichia coli and Streptococcus acuminatum, the main pathogens of endometritis in dairy cows, as well as the swelling of auricle and toes in mice, The permeability of celiac capillaries and the anti-inflammatory effect of granulomatous inflammatory model provide the theoretical basis for optimizing the traditional Chinese medicine compound "Gong Yan Jing". Methods: 1. The crude extracted Chinese medicine compound "Gong Yan Jing" (Leonurus chinensis, Ligusticum chuanxiong, Angelica sinensis) was prepared. Peach kernel, licorice, WuLingzhi, dried ginger, fuhuang) were added to each drug to determine whether there were alkaloids in each drug, such as potassium mercuric iodide, potassium bismuth iodide, silicotungstic acid and phosphomolybdic acid. The primary screening of each single drug containing alkaloids was carried out, The single medicine containing alkaloids was prepared as simplified compound compound according to the original dosage. The compound was extracted by ethanol reflux extraction method and ultrasonic assisted extraction method respectively. Alkaloids in Herba Leonurus mongolicum. The alkaloids in Herba Leonurus chinensis were determined by plate perforation method and double dilution method of test tube to simplify and simplify the alkaloids of Herba Leonurus to staphylococcus aureus, the main pathogen of endometritis of dairy cow. No Streptococcus lactobacillus, Escherichia coli in vitro bacteriostatic effect .4. by establishing mouse models of auricle swelling, toe swelling, celiac capillary permeability and granulomatous inflammation to evaluate the compound "Gong Yan Jing" and simplify the compound, The antiinflammatory effect of alkaloids in Herba Leonurus chinensis. Results 1. The crude extracts of 8 kinds of Chinese medicine compound "Gong Yan Jing" were tested with alkaloid reagent respectively. It was found that the alkaloids may exist in Leonurus, Ligusticum chuanxiong and Glycyrrhiza uralensis. After further refining, the above three drugs were confirmed to contain alkaloids, and the three drugs were made up of Leonurus chinensis, Ligusticum chuanxiong and Glycyrrhiza uralensis according to the dosage of the original prescription to simplify compound compound .2.The results were found by comparing the ethanol reflux extraction method with the ultrasonic assisted extraction method. The extraction rates of alkaloids in Herba Leonurus and ethanol reflux extraction were 0.92% and 0.73%, respectively. The extraction rate of ultrasonic assisted extraction method was 0.87%, 0.86%, 0.62% and 0.32%, respectively. The in vitro bacteriostasis test was carried out. It was found that the traditional Chinese medicine compound "Gong Yan Jing", simplified compound, Leonurus alkaloids in Staphylococcus aureus, The average bacteriostasis diameters of Escherichia coli and Streptococcus lactobacillus were 17.4 鹵0.8mm, 16.9 鹵1.2mm and 7.8 鹵0.8mm, respectively. The MBC of these three groups of alkaloids to Staphylococcus aureus were 32 g / L 125g / L ~ 125g / L ~ (125) g 路L ~ (-1) MIC62.5 g / L ~ (125) g / L ~ (500) g 路L ~ (-1) respectively; for Escherichia coli, MBC was 62.5 g / L ~ (125) g / L ~ (125) g / L ~ (125) g / L ~ (125) g / L, 500 g / L, respectively; for Streptococcus acinaceus, it was 250 g / L ~ 125 g / L, 500 g / L, respectively. 125 g / L, 125 g / L, 500 g / L, 250 g / L, 250 g / L, not measured .4.The anti-inflammatory test in mice showed that the compound compound "Gong Yan Jing", simplified alkaloids in compound compound and Leonurus, both caused swelling of auricle in mice. Foot swelling, celiac capillary permeability and the formation of cotton ball granuloma can be inhibited. The anti-inflammatory effect of compound "Gong Yan Jing" and simplified compound is significantly different from that of Leonurus alkaloid group (P 0.05), but not to dexamethasone group (P 0.05). The anti-inflammatory effect of simplified compound is slightly better than that of compound "Gong Yan Jing". Conclusion: simplified compound is better than that of compound "Gong Yan Jing". And the bacteriostatic effect of "Gong Yan Jing", a traditional Chinese medicine compound, in vitro, The anti-inflammatory activity in vivo is not different, which provides a theoretical basis for simplifying the clinical application of compound.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S853.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 郭瑞霞;李力更;王于方;王磊;張Z誒，

本文編號(hào)：1628217