發(fā)布時(shí)間：2018-03-14 21:38

  本文選題：高通量測(cè)序　切入點(diǎn)：腸道菌群　出處：《江西農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：為探討重金屬對(duì)海蘭褐蛋雞盲腸結(jié)構(gòu)和微生物多樣性的影響。實(shí)驗(yàn)選取30日齡海蘭褐蛋雞72羽,隨機(jī)分成4組:對(duì)照組(CK)、銅組(Cu)、汞組(Hg)和鉛組(Pb),每組3個(gè)重復(fù),每個(gè)重復(fù)6羽。CK組飼喂純凈水,Cu組飼喂含量為150mg/L CuCl2純凈水,Hg組為含量140mg/L HgCl2純凈水,Pb組為含量350 mg/L PbCl2純凈水。于實(shí)驗(yàn)第30d、60d、90d采取雞盲腸及盲腸內(nèi)容物。觀察雞盲腸組織結(jié)構(gòu)和應(yīng)用Illlumina-Hiseq 250高通量測(cè)序技術(shù)分析微生物群落結(jié)構(gòu)和多樣性的變化。結(jié)果如下:1、CK組盲腸組織學(xué)結(jié)構(gòu)完整,黏膜分布規(guī)律,隱窩處腺體發(fā)育正常。與對(duì)照組相比,Cu組、Hg組有少量黏膜脫落并伴有少量淋巴細(xì)胞增生,Pb組腸黏膜大量斷裂脫落并伴有大量淋巴細(xì)胞增生。2、72羽海蘭褐蛋雞盲腸微生物共得到5527565個(gè)有效序列,2431個(gè)OTUs,分類屬于28個(gè)門,348個(gè)屬。3、共享核心菌門主要為厚壁菌門、擬桿菌門、疣微菌門、變形菌門和柔膜菌門。屬水平以擬桿菌屬比例最高,占19.20%,其次是鉤藤菌屬。4、Cu組后壁菌門,乳酸菌及糞球菌等優(yōu)勢(shì)菌門及菌屬豐度最高。5、Hg組柔膜菌門,魏氏梭菌屬和丹毒絲菌屬等致病菌豐度最高。6、Pb組擬桿菌門、后壁菌門等優(yōu)勢(shì)菌門豐度降低;魏氏梭菌屬、丹毒絲菌屬、普雷沃氏菌屬等致病菌屬豐度升高。結(jié)果說明,重金屬對(duì)雞盲腸組織有一定的損傷作用,并影響腸道菌群的結(jié)構(gòu)及多樣性。
[Abstract]:In order to study the effects of heavy metals on the cecal structure and microbial diversity of Hailan brown laying hens, 72 30 day-old Hayland brown laying hens were randomly divided into four groups: control group, copper group, mercury group (Hgg) and lead group (3 replicates in each group). Each repeated 6 plume. CK group was fed with pure water containing 150 mg / L CuCl2 pure water and Hg group with a concentration of 140 mg / L HgCl2 pure water pure water Pb group was fed with 350 mg/L PbCl2 pure water. The contents of chicken caecum and cecum were taken on the 30th day of the experiment, and the contents of cecum and cecum were observed in the chicken caecum group. The changes of microbial community structure and diversity were analyzed by high-throughput sequencing technique of Illlumina-Hiseq 250. The results were as follows: the structure of cecum was intact in the 1: 1 CK group. Mucosal distribution, Compared with the control group, there was a small amount of mucosal exfoliation and a small amount of lymphocyte proliferation. The intestinal mucosa in Pb group was ruptured and exfoliated with a large number of lymphocyte proliferation. A total of 5527565 active sequences were obtained, including 2431 OTUs, belonging to 348 genera of 28 phylum. Bacteroid phylum, Microphylum verrucum, Proteus and Fleuromycetes. The highest proportion of Bacteroides was found in genus 19.20, followed by phylum of posterior wall in group of Leptacterium, Lactobacillus and faecal coccus, and the highest abundance of Bacteroides in the group of 5.5Hg, the second was the phylum of the posterior wall of the genus Leptacterium, the other dominant bacteria such as lactobacillus and faecococcus. The abundance of pathogenic bacteria such as Clostridium Weissii and Rhizoma erysipelas is the highest. The abundance of Bacteroides phylum and phylum of the posterior wall is decreased, the abundance of pathogenic bacteria such as Clostridium Weissii, Staphylococcus erysipelis, Genus Pravo is increased, the results show that, Heavy metals can damage the cecum tissues and affect the structure and diversity of intestinal flora.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S859.8

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