本文選題：豬繁殖與呼吸綜合征病毒　切入點(diǎn)：ORF　出處：《河南農(nóng)業(yè)科學(xué)》2017年10期 　論文類型：期刊論文

【摘要】：為了解華北地區(qū)豬繁殖與呼吸綜合征病毒(PRRSV)的遺傳變異情況,通過對2014—2016年從河南、河北、山東、山西4省采集的疑似豬繁殖與呼吸綜合征(PRRS)病料進(jìn)行檢測,將擴(kuò)增得到的PRRS陽性樣品的ORF5基因進(jìn)行測序,分析華北地區(qū)2014—2016年P(guān)RRSV的變異情況。結(jié)果表明,2014—2016年華北地區(qū)流行毒株與國內(nèi)外代表性毒株核苷酸同源性在84.6%~99.8%;抗原位點(diǎn)比對結(jié)果顯示,變異株中和表位第37—44位氨基酸為SH(I/L/F)QLIY(N/K),誘騙表位27—30位氨基酸為(V/A)L(V/A)N;系統(tǒng)進(jìn)化樹分析表明,以CH-1a為代表的變異株、以VR-2332為代表的經(jīng)典毒株以及NADC30毒株在華北地區(qū)同時存在�？梢�,華北地區(qū)PRRSV流行情況復(fù)雜。
[Abstract]:In order to understand the genetic variation of porcine reproductive and respiratory syndrome virus (PRRSVV) in North China, the suspected PRRSs collected from Henan, Hebei, Shandong and Shanxi provinces from 2014-2016 were detected. The ORF5 gene of the amplified PRRS positive samples was sequenced, and the variation of PRRSV from 2014 to 2016 in North China was analyzed. The results showed that the nucleotide homology of the prevalent strains in North China from 2014-2016 to that of the representative strains at home and abroad was 84.6% 99.8, and the results of antigen locus comparison showed that, The amino acids of the 37-44 amino acids in the neutralizing epitope of the mutant strain were SHI / L / FQLIY / N / KN, and the amino acids at the 27-30 amino acids of the decoy epitope were V / A / L / V / AN. The phylogenetic tree analysis showed that the variant strain represented by CH-1a, the classical strain represented by VR-2332 and the strain of NADC30 virus existed simultaneously in North China. The prevalence of PRRSV in North China is complicated.
【作者單位】： 河南省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;
【基金】：河南省科技攻關(guān)項(xiàng)目(162102110050,162102110042) 河南省農(nóng)業(yè)科學(xué)院自主創(chuàng)新基金項(xiàng)目(2016ZC51,2017ZC49)
【分類號】：Q78;S852.65
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本文編號：1610865