本文選題：耐藥菌　切入點：β-內(nèi)酰胺酶抑制劑　出處：《中國預(yù)防獸醫(yī)學(xué)報》2017年09期 　論文類型：期刊論文

【摘要】：為建立用于抗耐藥菌的β-內(nèi)酰胺酶(β-lactamase)抑制劑快速篩選方法,本研究采用雙層平板法,以2%的瓊脂為底層,1%的含碘試劑為上層,以青霉素為β-內(nèi)酰胺酶的底物,克拉維酸作為體系驗證的陽性對照,通過比較透明圈形成大小,優(yōu)化反應(yīng)體系,并對190個微生物發(fā)酵產(chǎn)物進行了初步篩選。研究最終確定的篩選條件為,pH7.0的PBS緩沖液體系,底物青霉素(160萬單位)濃度為50 mg/mL,β-內(nèi)酰胺酶濃度為10 U/mL,40℃孵育40 min。通過測量反應(yīng)體系透明圈形成大小來篩選具有活性的β-內(nèi)酰胺酶抑制劑。結(jié)果證實該方法可以為耐藥菌β-內(nèi)酰胺酶抑制劑的篩選提供快速簡單、易行、穩(wěn)定、可靠的研究平臺,并初步篩選獲得了具有較高β-內(nèi)酰胺酶抑制活性的化合物。
[Abstract]:In order to establish a rapid screening method of 尾 -lactamase inhibitors for resistant bacteria, a double-layer plate method with 2% Agar as the bottom layer and 1% iodine containing reagent as the top layer and penicillin as the substrate of 尾 -lactamase was studied. Clavulanic acid was used as a positive control to verify the system. By comparing the size of transparent circle, the reaction system was optimized, and 190 microbial fermentation products were screened. The final screening conditions were determined as PBS buffer system with pH 7.0. The concentration of penicillin (1.6 million units) was 50 mg / mL, and the concentration of 尾 -lactamase was 10 U / mL at 40 鈩，

本文編號：1605361