發(fā)布時間：2018-03-05 16:35

  本文選題：豬　切入點：JHDMA基因　出處：《中國畜牧獸醫(yī)》2017年06期 　論文類型：期刊論文

【摘要】：試驗旨在克隆豬JHDM2A基因,并研究其在豬卵巢組織中的表達情況。首先克隆豬JHDM2A基因,并構建pLVX-IRES-ZsGreen1-JHDM2A真核表達載體,同時對JHDM2A基因在豬卵泡發(fā)育過程中的表達情況進行分析。結果顯示,克隆得到的豬JHDM2A基因編碼區(qū)長度為3 945bp,編碼1 315個氨基酸。通過多重氨基酸序列比對發(fā)現,豬JHDM2A基因與黃牛、水牛、綿羊和人相應氨基酸序列的同源性分別為93.5%、94.7%、94.7%和93.8%。蛋白質分子系統(tǒng)進化樹分析結果表明,JHDM2A基因在物種進化過程中高度保守。通過脂質體轉染法將構建的pLVX-IRES-ZsGreen1-JHDM2A真核表達載體導入HEK293T細胞,可觀察到清晰的綠色熒光蛋白表達。免疫組化結果顯示,JHDM2A蛋白在不同發(fā)育階段豬卵泡中均有表達。本試驗通過克隆獲得豬JHDM2A基因序列,JHDM2A蛋白在豬卵巢中高度表達,表明其功能可能與豬卵泡發(fā)育密切相關。
[Abstract]:The aim of this study was to clone porcine JHDM2A gene and study its expression in porcine ovary. Firstly, porcine JHDM2A gene was cloned and eukaryotic expression vector of pLVX-IRES-ZsGreen1-JHDM2A was constructed. At the same time, the expression of JHDM2A gene in the development of pig follicle was analyzed. The results showed that the encoding region of the cloned porcine JHDM2A gene was 3 945 BP, encoding 1 315 amino acids, and was found by multiple amino acid sequence alignment. Pig JHDM2A gene and yellow cattle, water buffalo, The homology of corresponding amino acid sequences between sheep and human was 94.5% and 93.8%, respectively. The results of phylogenetic tree analysis showed that the JHDM2A gene was highly conserved in the evolution of species. The constructed pLVX-IRES-ZsGreen1-JHDM2A was expressed in eukaryotic by liposome transfection. The vector was introduced into HEK293T cells. A clear expression of green fluorescent protein was observed. Immunohistochemical results showed that JHDM2A protein was expressed in pig follicles at different stages of development. In this study, the pig JHDM2A gene sequence, JHDM2A protein, was highly expressed in porcine ovaries. It suggests that its function may be closely related to the development of pig follicles.
【作者單位】： 廣西大學亞熱帶農業(yè)生物資源保護與利用國家重點實驗室;
【基金】：國家自然科學基金(31560632) 廣西自然科學基金項目(2014GXNSFAA118099、2016GXNSFDA380030)
【分類號】：Q78;S828
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本文編號：1571050