本文選題：大腸桿菌　切入點(diǎn)：頭孢噻呋　出處：《黑龍江畜牧獸醫(yī)》2017年05期 　論文類型：期刊論文

【摘要】：為了探討體外頭孢噻呋單用及與甲氧芐啶聯(lián)用對(duì)致病性牛源大腸桿菌防突變濃度(MPC)的影響,試驗(yàn)選取對(duì)頭孢噻呋敏感的8株致病性牛源大腸桿菌,采用微量肉湯稀釋法和棋盤(pán)法測(cè)定頭孢噻呋與甲氧芐啶單藥及聯(lián)合后的最小抑菌濃度(MIC),計(jì)算部分抑菌濃度指數(shù)(FIC指數(shù)),判斷兩藥的相互作用;采用瓊脂平板稀釋法測(cè)定單藥及聯(lián)合后的MPC,并計(jì)算相應(yīng)的選擇指數(shù)(SI,SI=MPC/MIC)。結(jié)果表明:頭孢噻呋與甲氧芐啶的FIC指數(shù)為0.25~1,兩藥聯(lián)用呈協(xié)同或相加作用;頭孢噻呋單用的MPC為64~512μg/m L,SI為64~256;與甲氧芐啶聯(lián)合使用后MPC為16~32μg/m L,SI下降為8~64。說(shuō)明頭孢噻呋與甲氧芐啶聯(lián)用可降低頭孢噻呋單用對(duì)致病性牛源大腸桿菌的防突變濃度,縮小耐藥突變選擇窗。
[Abstract]:In order to investigate the effect of ceftiofuroxime alone and in combination with trimethoprim on MPCs of pathogenic bovine Escherichia coli, eight strains of pathogenic bovine Escherichia coli sensitive to cefotaxime were selected. The minimal inhibitory concentration of ceftiofuroxime and trimethoprim was determined by broth dilution method and chessboard method. The partial inhibitory concentration index and FIC index were calculated to judge the interaction between the two drugs. The single and combined MPCs were determined by Agar plate dilution method, and the corresponding selection index was calculated. The results showed that the FIC index of ceftiofuroxime and trimethoprim was 0. 25 ~ 1, which was synergistic or additive. The MPC of ceftiofuroxime alone was 64 / 512 渭 g / m ~ (-1) / L ~ (-1) and that of trimethoprim was 16 ~ (32) 渭 g / m ~ (-1) / L ~ (-1). The results showed that ceftiofuroxime combined with trimethoprim could reduce the anti-mutation concentration of ceftiofuroxime alone on pathogenic bovine Escherichia coli. Narrow the window of drug resistance mutation selection.
【作者單位】： 東北農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)學(xué)院;
【基金】：國(guó)家重點(diǎn)研發(fā)計(jì)劃專項(xiàng)(2016YFD051310) 黑龍江省自然科學(xué)基金面上項(xiàng)目(C2015020)
【分類號(hào)】：S852.612

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