本文關(guān)鍵詞： 馬屬動(dòng)物 腸道寄生蟲 流行病學(xué)調(diào)查 隱孢子蟲 MLST 群體遺傳結(jié)構(gòu)　出處：《河南農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：馬屬動(dòng)物寄生蟲病是現(xiàn)代馬業(yè)中最常見(jiàn)的一種慢性、隱蔽性疾病,患病動(dòng)物往往無(wú)明顯的臨床癥狀,嚴(yán)重時(shí)表現(xiàn)為消瘦、貧血、腹瀉,甚至是死亡。引起馬屬動(dòng)物寄生蟲病的病原主要有圓線蟲、馬副蛔蟲、絳蟲、球蟲、隱孢子蟲、賈第蟲等,其中隱孢子蟲和賈第蟲是全球分布的人獸共患原蟲病原,可感染多種動(dòng)物,在世界范圍內(nèi)廣泛流行。為了解我國(guó)部分地區(qū)驢腸道寄生蟲感染及流行情況,本研究于2013年3月至2014年5月對(duì)養(yǎng)驢相對(duì)集中的山東省嘉祥縣牲畜交易市場(chǎng)進(jìn)行驢腸道寄生蟲流行病學(xué)調(diào)查。本次調(diào)查共采集驢糞便樣品508份,采用盧戈氏碘液染色法和飽和蔗糖漂浮法進(jìn)行檢測(cè),檢出寄生蟲陽(yáng)性糞樣246份,總感染率為48.43%。共發(fā)現(xiàn)8種腸道寄生蟲,分別為:球蟲、賈第蟲、隱孢子蟲、圓線蟲、蛔蟲、絳蟲、鞭蟲、鉤蟲。其中以圓線蟲感染率最高,為32.48%(165/508),其次是蛔蟲,感染率為20.28%(103/508),球蟲、賈第蟲、隱孢子蟲、絳蟲、鞭蟲、鉤蟲感染率分別為3.74%(19/508)、0.39%(2/508)、5.91%(30/508)、2.76%(14/508)、0.59%(3/508)、0.20%(1/508)。本次調(diào)查發(fā)現(xiàn)驢腸道寄生蟲混合感染感染率為16.14%(82/508),其中以兩種寄生蟲混合感染為主,圓線蟲和另一種寄生蟲混合感染居多。為解析驢源隱孢子蟲的種系發(fā)育關(guān)系及群體遺傳結(jié)構(gòu),本試驗(yàn)選用HSP70、MSC6-7、MSC6-5、CP47、CP56、ML2、Chom3T、RPGR 8個(gè)基因位點(diǎn)對(duì)本實(shí)驗(yàn)室保存的114份驢源隱孢子蟲陽(yáng)性樣品進(jìn)行多位點(diǎn)擴(kuò)增。首先用GP60基因位點(diǎn)鑒定亞型,成功擴(kuò)增出69個(gè)分離株,包括18個(gè)C.parvum(Ⅱd A19G1)分離株,2個(gè)Horse genotype分離株和49個(gè)Donkey genotype(ⅫA16G1)分離株。經(jīng)過(guò)篩選,CP47和CP56位點(diǎn)擴(kuò)增效果不好,舍棄這兩個(gè)位點(diǎn)。共有14個(gè)隱孢子蟲Donkey genotype樣品在所有6個(gè)位點(diǎn)(Chom3T、HSP70、ML2、MSC6-5、MSC6-7、RPGR)全部有擴(kuò)增結(jié)果,用多位點(diǎn)序列分型(MLST)方法分析隱孢子蟲Donkey genotype群體遺傳結(jié)構(gòu)。結(jié)果表明,14個(gè)隱孢子蟲Donkey genotype分離株在上述6個(gè)基因位點(diǎn)分別形成2、1、3、4、4、3個(gè)亞型且形成7個(gè)MLST亞型,連鎖不平衡性分析結(jié)果表明此次調(diào)查的隱孢子蟲Donkey genotype為流行性群體結(jié)構(gòu),暗示驢隱孢子蟲病在馬屬動(dòng)物群體中的流行是不穩(wěn)定地的。
[Abstract]:Equine parasitosis is one of the most common chronic and hidden diseases in modern horse industry. The disease animals often have no obvious clinical symptoms. In severe cases, they are characterized by emaciation, anemia and diarrhea. Even death. The main pathogens causing parasitic diseases in equine animals are nematode, ascaris equina, tapeworm, coccidia, Cryptosporidium, giardia, among which Cryptosporidium and Giardia are the worldwide zoonotic protozoan pathogens. In order to understand the infection and epidemic situation of donkey intestinal parasites in some parts of China, From March 2013 to May 2014, an epidemiological survey of donkey intestinal parasites was carried out in the livestock market of Jiaxiang County, Shandong Province, where donkey breeding was relatively concentrated. A total of 508 donkey feces samples were collected in this study. Two hundred and forty-six parasite positive feces were detected by Lugo's iodine solution staining and sucrose floatation. The total infection rate was 48.43. Eight intestinal parasites were found, which were coccidia, giardia, Cryptosporidium, nematode, ascariasis. The infection rate of tapeworm, Trichuris and hookworm was the highest, 32.48% 165 / 508%, followed by Ascaris lumbricoides, and the infection rate was 20.280.103% 508%, coccidia, giardia, Cryptosporidium, tapeworm, Trichuris, The infection rate of hookworm is 3.74 / 508 / 0.390.39 and 5.91 / 5.91 / 3.76 / 5080.59 / 5080.59 / 5080.20 / 5080.20% / 508. this survey found that the infection rate of mixed infection of intestinal parasites in donkey is 16.14% 822% 508%, in which the two parasites are mainly mixed infection. In order to elucidate the phylogenetic relationship and population genetic structure of Cryptosporidium donkeys, nematode and another parasite were most commonly infected. In this experiment, 8 loci of HSP70 / MSC6-7 / CP47 / CP56 / ML2 / Chom3TGR were used to amplify 114 samples of Cryptosporidium donkeys. First, 69 isolates were successfully amplified by using GP60 gene loci to identify the subtype of Cryptosporidium donkeys. There were 18 C.parvum (鈪，

本文編號(hào)：1547993