吉林省活禽市場外環(huán)境禽流感污染情況調查
發(fā)布時間:2018-02-28 08:23
本文關鍵詞: 活禽市場 禽流感 吉林省 出處:《中國公共衛(wèi)生》2017年03期 論文類型:期刊論文
【摘要】:目的目的了解吉林省活禽市場外環(huán)境禽流感病毒分布狀況。方法于2014年3月1日—10日采集吉林省活禽市場外環(huán)境,共抽取370個采樣點,每個點抽取50家活禽攤位采集外環(huán)境雞籠涂抹、雞糞和污水標本,采用實時熒光定量PCR法檢測禽流感病毒FluA H5、H9和H7核酸。結果 370個采樣點中58個(15.68%)檢出陽性標本,各地區(qū)標本核酸陽性率有明顯差異(P0.05);共采集活禽市場外環(huán)境標本1 110份,分別為污水359份,糞便381份,籠具擦拭物370份,其中陽性94份,H9陽性60份,H5陽性30份,H5/H9混合陽性4份,未檢測出H7陽性,不同類型標本的陽性檢出率無統(tǒng)計學差異。結論吉林省活禽市場環(huán)境存在禽流感病毒的污染,主要型別為H9和H5亞型,未發(fā)現(xiàn)H7亞型。
[Abstract]:Objective to understand the Jilin province live poultry market environment of avian influenza virus distribution. Methods from March 1, 2014 - 10 day gathering in Jilin province live poultry market environment, collected a total of 370 sampling points, each point selected 50 live poultry stalls collection environment cages smear, chicken manure and sewage samples, by using real-time fluorescence quantitative PCR method detection of avian influenza virus FluA, H5, H9 and H7 nucleic acid. The results of 370 sampling points in 58 (15.68%) were positive, the positive rate of nucleic acid samples have obvious difference regions (P0.05); collected live poultry market environment 1110 samples respectively, 359 sewage, 381 fecal specimens of cage cleaning 370, of which 94 samples were positive, 60 were H9 positive, 30 were positive for H5, H5/H9 mixed positive 4, did not detect H7 positive, the positive rate of different types of samples, no significant difference. Conclusion Jilin province live poultry market environment contamination of avian influenza virus, the main The type of H9 and H5 subtype was not found in H7 subtype.
【作者單位】: 吉林省疾病預防控制中心傳染病預防控制所;吉林省衛(wèi)生監(jiān)測檢驗中心;
【基金】:吉林省自然科學基金(20140101031JC) 吉林省重點科技攻關項目(20150204005SF)
【分類號】:S855.3
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相關期刊論文 前5條
1 盧珊;陳晨;于偉文;王海印;杜鵬程;闞飆;徐建國;;利用網絡爬蟲技術分析我國活禽貿易與H7N9禽流感病毒傳播的關系[J];中華流行病學雜志;2014年03期
2 孫曉強;溫秋芳;馬學e,
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