本文關(guān)鍵詞： 馬崗鵝 公雞 促性腺抑制激素 繁殖性能 生殖發(fā)育　出處：《仲愷農(nóng)業(yè)工程學(xué)院》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：Gn IH可以作用于垂體前葉或作用于Gn RH來(lái)抑制促性腺激素的合成與釋放,是調(diào)節(jié)動(dòng)物繁殖性能的重要生殖激素之一。目前用生殖免疫調(diào)控的方法提高家畜繁殖力已受到廣泛關(guān)注。許多研究應(yīng)用抑制素、催乳素、Gn IH等生殖激素作用于動(dòng)物體內(nèi)或體外實(shí)驗(yàn)均可發(fā)現(xiàn)其參與調(diào)控動(dòng)物的生長(zhǎng)繁殖。因此,本實(shí)驗(yàn)主要以馬崗鵝和公雞為研究對(duì)象,從外源性給予Gn IH和主動(dòng)免疫Gn IH兩種不同的研究方法進(jìn)行研究:實(shí)驗(yàn)一選取健康的成年母鵝36只,平均分成3組。第一組僅在d1給予30%PVP-K30溶解的Gn IH溶液,給予Gn IH 1 mg/只;第二組在d1、d4、d7給予生理鹽水溶解的Gn IH溶液,給予Gn IH 1 mg/只;第三組在d1、d4、d7給予生理鹽水,給予1 m L每只。試驗(yàn)中記錄母鵝每日產(chǎn)蛋數(shù)和就巢情況;并在d1、d4、d7、d10定時(shí)采集血清,在試驗(yàn)結(jié)束時(shí)屠宰母鵝,采集下丘腦和垂體組織樣和檢查卵泡發(fā)育情況。研究表明給予產(chǎn)蛋母鵝外源性Gn IH能一定程度上抑制自身Gn IH的基因表達(dá),降低血液中LH濃度,提高雌二醇濃度,主要通過(guò)抑制中小卵泡發(fā)育,進(jìn)而在一定程度上抑制母鵝產(chǎn)蛋。實(shí)驗(yàn)二選取30只處于就巢期健康的成年馬崗鵝母鵝,平均分成免疫組和對(duì)照組(n=15),試驗(yàn)在正式開(kāi)始時(shí),對(duì)母鵝進(jìn)行人工強(qiáng)制終止就巢。免疫組分別在d1、d21、d41接種含Gn IH蛋白的疫苗1 m L/只(以此含1.0、1.0、0.5 mg的Gn IH蛋白),對(duì)照組在d1、d21、d41注射未含免疫原的生理鹽水制備的乳化苗。兩組鵝試驗(yàn)開(kāi)始飼養(yǎng)于短光照(11L:13D)下以繼續(xù)維持正常產(chǎn)蛋,在d72將每天的光照時(shí)間延長(zhǎng)至18小時(shí)(18L:6D)至d95試驗(yàn)結(jié)束。試驗(yàn)期記錄鵝的就巢發(fā)生、每日產(chǎn)蛋數(shù),并每20天對(duì)母鵝采血一次,分離血清留待激素測(cè)定。在試驗(yàn)結(jié)束時(shí)對(duì)兩組母鵝進(jìn)行屠宰,檢查卵泡發(fā)育情況,并采集下丘腦和垂體樣本,留待基因表達(dá)水平檢測(cè)。研究結(jié)果表明對(duì)成年母鵝主動(dòng)免疫Gn IH可誘導(dǎo)體內(nèi)產(chǎn)生特異性抗Gn IH的抗體,抑制Gn IH的基因表達(dá),略微提高了Gn RH、VIP和PRL的基因表達(dá),調(diào)節(jié)血清中LH、E2、P4等相關(guān)生殖激素進(jìn)而提高母鵝的產(chǎn)蛋性能。另外,延長(zhǎng)光照會(huì)抑制母鵝的產(chǎn)蛋,但其對(duì)對(duì)照組的作用比免疫組更強(qiáng),這可能是因?yàn)橹鲃?dòng)免疫Gn IH對(duì)母鵝的產(chǎn)蛋促進(jìn)作用還在維持。實(shí)驗(yàn)三選120只60日齡健康公雞,隨機(jī)平均分成兩組,免疫組和對(duì)照組,所有雞均采用籠養(yǎng),每籠2只。免疫組接種含Gn IH蛋白的疫苗1 m L/只(含0.5 mg的Gn IH蛋白),對(duì)照組注射未含免疫原的生理鹽水制備的乳化苗。分別在試驗(yàn)的d1、d21和d41(即60、80和100日齡)進(jìn)行胸部肌肉注射接種疫苗。在試驗(yàn)期,從60日齡開(kāi)始,每隔20天進(jìn)行對(duì)公雞進(jìn)行采血,所得血清保存于-20℃,留待測(cè)定血液中抗體水平和激素水平;每隔30天分別從兩組隨機(jī)挑選8只公雞進(jìn)行屠宰,采取睪丸組織并檢測(cè)其發(fā)育情況,并采集下丘腦和垂體留待基因表達(dá)水平檢測(cè)。從195日齡開(kāi)始兩組公雞進(jìn)行人工采精訓(xùn)練(按摩法),待一周建立條件反射后,每隔兩天采精一次進(jìn)行精液品質(zhì)檢測(cè)。研究結(jié)果表明,對(duì)發(fā)育期公雞主動(dòng)免疫Gn IH能有效的刺激了機(jī)體產(chǎn)生特異性抗Gn IH抗體,并在整個(gè)實(shí)驗(yàn)過(guò)程中維持較高水平。免疫Gn IH后能抑制Gn IH、VIP、PRL的基因表達(dá),提高LH的基因表達(dá),進(jìn)而促進(jìn)睪丸發(fā)育和血液循環(huán)中睪酮、INH、ACT的分泌,提高精液品質(zhì)。
[Abstract]:Gn IH can function in the anterior pituitary function in Gn or RH to inhibit the synthesis and release of gonadotropin, is one of the most important reproductive hormone regulation of animal reproductive performance. The current method for reproductive immune regulation to improve the fecundity of cattle has attracted widespread attention. Many studies by prolactin inhibin, Gn, IH and other reproductive hormones in animal in vivo or in vitro experiments can be found which is involved in the regulation of animal growth and reproduction. Therefore, this experiment mainly in Magang geese and cock as the research object, Gn IH and Gn IH active immunization with two different research methods from exogenous research: the first experiment healthy adult female geese 36, divided into 3 groups the first group was given 30%PVP-K30 dissolved in D1 Gn IH Gn IH mg/ 1 solution, given only; second in D1 group, D4 Gn, IH D7 solution of saline solution, given the Gn IH 1 mg/; third in D1 group, D4, D7 Saline, 1 m L each. The goose egg number per Nissan and nest record test; and in D1, D4, D7, D10 in serum collected at the end of the experiment, the slaughter of the goose, collecting the hypothalamus and pituitary tissue samples and check the follicular development. The results show that the goose egg exogenous Gn IH can inhibit the Gn gene expression of IH, reduce the LH concentration in the blood, improve the concentration of estradiol, mainly through the inhibition of small follicular development, thereby inhibiting the goose egg in a certain extent. In experiment two, a total of 30 healthy adult in broodiness period Magang goose goose, divided into immune group and control group (n=15), the test officially started, the goose was forced to terminate the broodiness. Artificial immune group respectively in D1, D21, d41 Gn IH protein vaccine inoculation with 1 m L/ only (mg Gn to 1.0,1.0,0.5 containing IH protein), the control group in D1, D21, d41 without injection Immunogenicity of saline prepared emulsion seedlings. Two groups of goose started to short photoperiod test (11L:13D) to continue to maintain normal production, the D72 daily illumination time is extended to 18 hours (18L:6D d95) to the end of the experiment. Recorded during the experiment of goose nest, daily egg number, and every 20 days for the goose blood once, for the separation and determination of serum hormone. The slaughter of two groups of goose at the end of the test, check the follicular development and acquisition of the hypothalamus and pituitary samples, for the detection of expression level of genes. The results of the study show that the antibody of adult goose Gn active immunization can induce the body to produce specific IH anti Gn IH, inhibit the expression of Gn IH gene, and slightly increased the Gn RH expression of VIP and PRL genes, regulating the serum levels of LH, E2, P4 and other related reproductive hormones and improve the performance of laying goose. In addition, extended light inhibition goose egg, but the The role of the two groups were stronger, this may be because the active immunization of Gn IH on the goose egg role still maintain. Experiment three choose 120 healthy 60 day old cocks were randomly divided into two groups, immune group and control group, all the chickens were used in cages, each cage 2 group. Immune vaccination Gn containing IH protein vaccine M 1 L/ (0.5 mg Gn IH protein), the control group was injected with saline without immunogen prepared emulsion vaccine. Respectively in the test of D1, D21 and d41 (60,80 and 100 days of age) chest muscle injection vaccination. In the trial period. From the beginning of the 60 days, every 20 days of blood serum of cock, stored in the -20 the C, determination of antibody levels and hormone levels in the blood for; every 30 days respectively from two randomly selected group of 8 cocks were slaughtered, take testicular tissue and detect its development situation, and the hypothalamic and pituitary for acquisition Gene expression detection. From 195 days of age two group cock artificial sperm collection training (massage), for a week after the establishment of conditioned reflex, once every two days of semen collection semen quality testing. The results show that can effectively stimulate the body to produce specific anti Gn IH antibody on the development period of the cock active immunization of Gn IH, and to maintain a high level during the whole experiment. Gn IH Gn can inhibit the immune IH, VIP, PRL gene expression, enhance the expression of LH gene, and promote testosterone, testis development and blood circulation in INH, ACT secretion, improve semen quality.

【學(xué)位授予單位】：仲愷農(nóng)業(yè)工程學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831;S835

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 趙悅;楊煥民;黃玉蘭;;家禽抑制素和活化素結(jié)構(gòu)與功能的研究進(jìn)展[J];黑龍江畜牧獸醫(yī);2007年02期

2 劉容珍;黃運(yùn)茂;李萬(wàn)利;田允波;施振旦;;光照對(duì)馬崗鵝卵泡發(fā)育及相關(guān)激素mRNA水平的影響[J];華南農(nóng)業(yè)大學(xué)學(xué)報(bào);2009年03期

相關(guān)碩士學(xué)位論文 前2條

1 秦緒光;中草藥復(fù)方添加劑對(duì)繁殖后期種公雞繁殖性能和血液生理生化指標(biāo)的影響[D];西北農(nóng)林科技大學(xué);2006年

2 夏東坡;野生雄性黃山短尾猴性腺激素與性行為關(guān)系研究[D];安徽大學(xué);2007年

，

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