本文關(guān)鍵詞： 繁殖母羊 妊娠 胎次 氧化應(yīng)激　出處：《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2017年03期 　論文類(lèi)型：期刊論文

【摘要】：本試驗(yàn)旨在探究不同生殖階段、胎次繁殖母羊的氧化應(yīng)激及氧化損傷狀態(tài)。試驗(yàn)1選取體重、體況相近的2~3胎空懷期、妊娠期(30、60、120 d)、分娩及哺乳期(21 d)的崇明白山羊各5只;試驗(yàn)2選取后備、1胎、2~3胎、4~5胎、6胎及以上的崇明白山羊母羊各5只。分別采集血樣,測(cè)定血清自由基代謝指標(biāo)[皮質(zhì)醇、過(guò)氧化氫(H2O2)含量]、抗氧化指標(biāo)[總抗氧化能力(T-AOC)、谷胱甘肽過(guò)氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性]、氧化損傷指標(biāo)[丙二醛(MDA)、8-異前列腺素-F2α(8-ISO-PGF2α)、8-羥基脫氧鳥(niǎo)苷(8-OHDG)、蛋白質(zhì)羰基化(PC)含量]。結(jié)果表明:試驗(yàn)1,從空懷期至哺乳期,血清皮質(zhì)醇、H2O2含量呈先上升后降低的趨勢(shì),在分娩時(shí)達(dá)最高值,較空懷期顯著提高(P0.05);血清T-AOC、SOD活性逐漸增強(qiáng),且哺乳期21 d較空懷期顯著提高(P0.05);血清MDA、8-ISO-PGF2α、PC含量先增加后趨于平穩(wěn),且在分娩時(shí)達(dá)最高,較空懷期顯著提高(P0.05),血清8-OHDG含量逐漸增加,哺乳期21 d達(dá)到最高,較空懷期顯著提高(P0.05)。試驗(yàn)2,隨著母羊胎次的增加,血清中皮質(zhì)醇含量無(wú)顯著變化(P0.05),4~5胎、6胎及以上血清H2O2含量顯著高于其他胎次(P0.05);血清TAOC無(wú)顯著變化(P0.05),血清SOD及GSH-Px活性逐級(jí)遞減,6胎及以上較后備母羊下降顯著(P0.05);血清MDA、8-ISO-PGF2α、8-OHDG和PC含量逐漸增加,6胎及以上顯著高于其他胎次(P0.05)。綜上所述,伴隨著妊娠的進(jìn)行,母羊體內(nèi)的自由基代謝逐漸增強(qiáng),與此同時(shí),體內(nèi)的抗氧化水平也相應(yīng)提高以維持氧化-抗氧化體系平衡,但在妊娠后期至哺乳期激增的活性氧自由基破壞了自由基穩(wěn)衡體系,導(dǎo)致氧化應(yīng)激和氧化損傷;隨著母羊胎次的增加,抗氧化能力逐漸減弱,高胎次母羊氧化損傷嚴(yán)重。
[Abstract]:The purpose of this experiment was to investigate the oxidative stress and oxidative damage state in different reproductive stages of breeding ewe. In experiment 1, 5 chonming white goats were selected for body weight, similar body condition (2), gestational period (30) and gestational period (60 ~ (120) d) and parturition and lactation for 21 days (n = 5). In experiment 2, the serum free radical metabolism index [cortisol] was determined by collecting blood samples from each of Chongming White Goat ewe, including 1, 1, 2, 2, 3, 4, 4, 4, 4, and 6, and above, of Chongming White Goat, and the serum free radical metabolism index [cortisol] was determined. Content of hydrogen peroxide H _ 2O _ 2], antioxidant index [total antioxidant capacity (T-AOCX), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)], oxidative damage index [malondialdehyde (MDAA) 8-isoprostaglandin F _ 2 偽 (8-ISO-PGF2 偽) -8-hydroxy-deoxyguanosine 8-OHDGN, protein, protein. The results showed that: test 1, from empty pregnancy to lactation, The content of H _ 2O _ 2 in serum increased first and then decreased, reached the highest value at delivery, increased the activity of T-AOCn SOD gradually, and increased significantly at 21 days after lactation. The content of serum MDA8-ISO-PGF2 偽 PC increased first and then tended to be stable. It reached the highest level during labor, significantly increased P0.05N, gradually increased serum 8-OHDG content, reached the highest level at 21 days of lactation, and significantly increased P0.05N during empty pregnancy period. Experiment 2, with the increase of fetal number of ewe, increased. There was no significant change in serum cortisol content. The content of H _ 2O _ 2 in serum of 6 or more fetus was significantly higher than that of other fetus (P 0.05), the serum TAOC was not changed significantly (P 0.05), and the activities of serum SOD and GSH-Px decreased gradually (P 0.05) in 6 fetuses and more than those in reserve ewe (P 0.05). The contents of MDA-8-ISO-PGF2 偽 8-OHDG and PC gradually increased in 6 or more embryos than those in other groups (P 0.05). Along with pregnancy, the metabolism of free radicals in ewe gradually increased, at the same time, the antioxidant level in the body was also increased in order to maintain the balance of oxidation-antioxidant system. However, the proliferation of reactive oxygen free radicals from late pregnancy to lactation resulted in oxidative stress and oxidative damage, which resulted in oxidative stress and oxidative damage. With the increase of birth order, the antioxidant capacity gradually weakened, and the oxidative damage of high parity ewe was serious.
【作者單位】： 上海交通大學(xué)農(nóng)業(yè)與生物學(xué)院上海市獸醫(yī)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;上海市崇明縣動(dòng)物疫病預(yù)防控制中心;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201303143-06-01)
【分類(lèi)號(hào)】：S826

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