本文關(guān)鍵詞： 醋酸棉酚 棉粕 紅平紅球菌 發(fā)酵 菌種鑒定　出處：《中國油脂》2016年04期 　論文類型：期刊論文

【摘要】：利用醋酸棉酚作為唯一碳源,篩選出一株高效降解棉酚的菌株RE-1。通過掃描電鏡和16S rRNA分子學(xué)鑒定該菌為紅平紅球菌(Rhodococcus erythropolis),Gen Bank序列登錄號(hào)為KR906527。掃描電鏡和透射電鏡觀察固體LB培養(yǎng)基和棉酚培養(yǎng)基中的RE-1菌株可知,棉酚會(huì)通過破壞菌體細(xì)胞膜通透性的方式來抑制RE-1的生長(zhǎng)。實(shí)驗(yàn)確定的棉粕最優(yōu)發(fā)酵條件為RE-1接種量20%、時(shí)間8 d、溫度34℃、p H 5.0、料水比1∶0.5,最優(yōu)條件下棉酚的降解率達(dá)到72.54%,棉粕中粗蛋白質(zhì)含量提高了21.38%,粗纖維含量降低了20.26%,釋放出營養(yǎng)物質(zhì),提高了棉粕的消化利用率。
[Abstract]:Gossypol acetate was used as the sole carbon source. A highly efficient gossypol degrading strain RE-1 was screened. The strain was identified by scanning electron microscopy and 16s rRNA molecular analysis as Rhodococcus erythropolisus Genin Bank accession number KR906527. The solid LB medium and gossypol medium were observed by scanning electron microscope and transmission electron microscope. The RE-1 strains in the. Gossypol can inhibit the growth of RE-1 by destroying cell membrane permeability. The optimum fermentation conditions of cottonseed meal are as follows: inoculation amount of RE-1 20, time 8 days, temperature 34 鈩，

本文編號(hào)：1531878