本文關(guān)鍵詞： 妊娠母鼠 血紅蛋白 鐵含量 鐵調(diào)素 血紅素鐵　出處：《動(dòng)物營養(yǎng)學(xué)報(bào)》2017年06期 　論文類型：期刊論文

【摘要】：本試驗(yàn)旨在探索血紅素鐵與硫酸亞鐵(FeSO_4)對妊娠母鼠繁殖成績,妊娠母鼠組織與胎鼠鐵含量,妊娠母鼠組織鐵調(diào)素(hepcidin)、膜鐵轉(zhuǎn)運(yùn)蛋白(Fpn)、貓白血病病毒C亞類受體(Flvcr)、轉(zhuǎn)鐵蛋白受體1(Tfr1)、轉(zhuǎn)鐵蛋白受體2(Tfr2)、二價(jià)金屬轉(zhuǎn)運(yùn)體1(DMT1)和血紅素轉(zhuǎn)運(yùn)蛋白(HCP)表達(dá)的影響。隨機(jī)選取2月齡體況接近的昆明小鼠母鼠80只,隨機(jī)分為8組,分別為對照組、缺鐵組、血紅素鐵組(15、60、90 mg/kg血紅素鐵)、FeSO_4組(75、300、450 mg/kg FeSO_4),每組10只。配種受孕起對照組飼喂正常飼糧(基礎(chǔ)飼糧中添加400 mg/kg FeSO_4);其他各組均飼喂基礎(chǔ)飼糧,妊娠第10~13天注射40 mg/kg去鐵胺(DFO),誘導(dǎo)妊娠母鼠缺鐵模型;妊娠第14天血紅素鐵組和FeSO_4組開始在基礎(chǔ)飼糧中添加血紅素鐵或FeSO_4,缺鐵組不添加。試驗(yàn)期為妊娠后1~20 d。結(jié)果表明:1)60 mg/kg血紅素鐵組胎鼠重最高,極顯著高于對照組與缺鐵組(P0.01)。2)60 mg/kg血紅素鐵組與450 mg/kg FeSO_4組母鼠血液血紅蛋白(HGB)含量、紅細(xì)胞數(shù)(RBC)和紅細(xì)胞容積(HCT)極顯著高于缺鐵組(P0.01)。3)60 mg/kg血紅素鐵組胎鼠鐵含量最高,極顯著高于對照組與FeSO_4組(P0.01);450 mg/kg FeSO_4組母鼠肝臟、脾臟和胎盤鐵含量均為最高。4)90 mg/kg血紅素鐵組和450 mg/kg FeSO_4組母鼠肝臟hepcidin表達(dá)量較高,極顯著高于缺鐵組與對照組(P0.01);15 mg/kg血紅素鐵組母鼠肝臟Fpn、Tfr2表達(dá)量較高,極顯著高于對照組和缺鐵組(P0.01);60 mg/kg血紅素鐵組母鼠肝臟Tfr1、Flvcr表達(dá)量較高,極顯著高于對照組和缺鐵組(P0.01);75 mg/kg FeSO_4組母鼠肝臟Tfr1表達(dá)量較高,極顯著高于對照組和缺鐵組(P0.01)。5)缺鐵組母鼠十二指腸Fpn、HCP、DMT1、Flvcr表達(dá)量均極顯著高于對照組(P0.01)。6)缺鐵組母鼠胎盤Fpn、Tfr1、DMT1、Flvcr、HCP表達(dá)量極顯著高于對照組(P0.01);90 mg/kg血紅素鐵組和450 mg/kg FeSO_4組母鼠胎盤hepcidin表達(dá)量較高,極顯著高于缺鐵組與對照組(P0.01)。7)飼糧血紅素鐵添加量為61.00 mg/kg或FeSO_4添加量為336.11 mg/kg時(shí),胎鼠鐵含量最高;飼糧血紅素鐵添加量為93.49 mg/kg時(shí),母鼠肝臟鐵含量最高。綜合得出,母鼠飼糧中添加適宜量的血紅素鐵或FeSO_4均可顯著促進(jìn)胎鼠增重,誘導(dǎo)母鼠靶組織鐵調(diào)基因的表達(dá),提高母鼠組織和妊娠20 d胎鼠機(jī)體鐵含量;HCP和Flvcr在母鼠腸道對血紅素鐵吸收或胎盤轉(zhuǎn)運(yùn)起至關(guān)重要的作用,但腸道吸收或胎盤轉(zhuǎn)運(yùn)FeSO_4主要以DMT1和Tfr2為主。關(guān)鍵詞:妊娠母鼠;血紅蛋白;鐵含量;鐵調(diào)素;血紅素鐵
[Abstract]:The aim of this study was to investigate the reproductive performance of pregnant rats, the tissue of pregnant rats and the content of iron in fetal mice by heme iron and FeSO4. The effects of ferritin hepcidinine, membrane iron transport protein (FpnnP), feline leukemia virus subclass C receptor (Flvcrn), transferrin receptor 1 (Tfr1), transferrin receptor 2 (Tfr2), divalent metal transporter 1 (DMT1) and heme transporter (HCP) were investigated. 80 female Kunming mice with similar body condition at 2 months of age, They were randomly divided into 8 groups: control group, iron deficiency group and heme iron group for 90 mg/kg heme iron and FeSO4 + 4 groups with 45 mg/kg FeSO4 and 10 rats in each group. The control group was fed with normal diet (400 mg/kg FeSO4 in basic diet), and all other groups were fed basic diet, and all other groups were fed with basic diet, and the other groups were fed with basic diet, and the control group was fed with normal diet (400 mg/kg FeSO4 +), and all the other groups were fed with basic diet, and all the other groups were fed with basic diet. On the 13th day of gestation, 40 mg/kg deferramide was injected to induce the model of iron deficiency in pregnant rats. On the 14th day of pregnancy, heme iron group and FeSO_4 group began to add heme iron or FeSO4 in basic diet, but iron deficiency group did not add heme iron. The contents of hemoglobin (HGB), erythrocyte count (RBC) and erythrocyte volume (RBC) in the heme iron group and the 450 mg/kg FeSO_4 group were significantly higher than those in the iron deficiency group and the iron deficiency group, and the highest iron content was found in the fetal rats in the iron deficiency group. The expression of hepcidin in liver, spleen and placenta was significantly higher than that in the control group and FeSO_4 group, and the expression of hepcidin was higher in the liver, spleen and placenta of the female rats than those in the control group and the FeSO_4 group. The expression of hepcidin in the liver of the female rats was significantly higher than that of the control group and the FeSO_4 group. It was significantly higher than that of iron deficiency group and control group for 15 mg/kg heme iron group. The expression of Fpnntf2 in liver of female rats was significantly higher than that of control group and iron deficiency group. The expression of Tfr1 flvcr in liver of female rats was significantly higher than that of control group and iron deficiency group for 60 mg/kg heme iron group, and the expression of Tfr1 flvcr in liver of female rats was significantly higher than that of control group and iron deficiency group for 60 mg/kg. The expression of Tfr1 in liver of female rats was significantly higher than that of control group and iron deficiency group. The expression of Tfr1 in liver of female rats was higher than that of control group and iron deficiency group. The expression of Fpnntrol / DMT1 / Flvcr in the duodenum of the iron-deficient group was significantly higher than that of the control group (P0.01 / 6).) the expression of Fpnnntrol Tfr1DMT1 / Flvcr-HCP in placenta of the iron-deficient group was significantly higher than that of the control group (P0.01 / 90 mg/kg heme iron group and 450 mg/kg FeSO_4 group, respectively.) the expression of Fpnntropium in the placental placenta of the iron-deficient group was significantly higher than that in the control group (P 0.01) and the female fetus in the 450 mg/kg FeSO_4 group was significantly higher than that in the control group. The expression of hepcidin was high. The iron content of fetal rats was the highest when dietary heme iron addition was 61.00 mg/kg or FeSO_4 addition was 336.11 mg/kg, and the highest iron content in maternal liver was obtained when dietary heme iron supplementation was 93.49 mg/kg, respectively, which was significantly higher than that in iron deficiency group and control group (P 0.01g 路7), and the content of heme iron in maternal liver was the highest when the content of heme iron was 61.00 mg/kg or 336.11 mg/kg. The addition of appropriate amount of heme iron or FeSO_4 in maternal diet could significantly promote the weight gain of fetal mice and induce the expression of iron regulatory gene in the target tissues of maternal mice. To increase the iron content in maternal tissues and fetal mice on the 20th day of gestation, HCP and Flvcr play an important role in the absorption of heme iron or placental transport in maternal intestines, but DMT1 and Tfr2 are the main components in intestinal absorption or placental transport of FeSO_4. Hemoglobin; iron content; iron modulin; heme iron
【作者單位】： 云南農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院云南省動(dòng)物營養(yǎng)與飼料重點(diǎn)試驗(yàn)室;
【基金】：云南省重大科技計(jì)劃——生物育種(2012ZA018-3)
【分類號】：S816

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