本文關(guān)鍵詞： 免疫應(yīng)答能力 細(xì)胞免疫 體液免疫 淋巴細(xì)胞增殖 支原體　出處：《河北工程大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：免疫系統(tǒng)是機(jī)體執(zhí)行免疫應(yīng)答及免疫功能的重要系統(tǒng)。有研究發(fā)現(xiàn),免疫反應(yīng)有25%的遺傳力。這一遺傳力和生產(chǎn)性能及體型遺傳力相類(lèi)似,充分利用免疫應(yīng)答能力較高的公牛,可以提高公犢利用率,使牛群整體免疫力普遍提高,并且可將這些更高免疫反應(yīng)遺傳給后代。本論文以優(yōu)良荷斯坦?fàn)俟檠芯繉?duì)象,針對(duì)細(xì)胞免疫和抗體免疫,采用流式細(xì)胞術(shù)、熒光定量PCR、淋巴細(xì)胞增殖及ELISA液相阻斷抗體檢測(cè)等試驗(yàn)方法,分析不同個(gè)體免疫應(yīng)答能力。根據(jù)其免疫應(yīng)答水平分為高免疫應(yīng)答個(gè)體與低免疫應(yīng)答個(gè)體,并對(duì)高免疫應(yīng)答能力個(gè)體及低免疫應(yīng)答能力個(gè)體間的差異性進(jìn)行分析。體液免疫試驗(yàn)通過(guò)ELISA液相阻斷抗體檢測(cè)不同個(gè)體免疫刺激后血清中抗體含量。結(jié)果表明,不同個(gè)體間抗體含量差異顯著;不同免疫時(shí)間其抗體含量差異較顯著。母源抗體較高的個(gè)體其一免刺激后抗體效價(jià)出現(xiàn)明顯下降;根據(jù)群體抗體效價(jià)篩選高抗體介導(dǎo)的免疫應(yīng)答個(gè)體�？谔阋呷N亞型間抗體效價(jià)較高的個(gè)體與抗體效價(jià)較低的個(gè)體進(jìn)行分析比較,A型抗體效價(jià)免疫刺激各階段差異較顯著。細(xì)胞免疫試驗(yàn)當(dāng)中,通過(guò)T淋巴亞群CD4~+CD8~+檢測(cè),發(fā)現(xiàn)群體免疫前后表達(dá)率差異不顯著;經(jīng)ConA刺激后,不同個(gè)體間表達(dá)率差異顯著;結(jié)果表明,增殖率較高的個(gè)體其免疫應(yīng)答能力較高,增殖率較低的個(gè)體其免疫應(yīng)答能力較低;熒光定量PCR試驗(yàn)使用T細(xì)胞有絲分裂原ConA和B細(xì)胞有絲分裂原LPS分別刺激后測(cè)定其Th1型細(xì)胞因子IL-2、IFN-γ和Th2型細(xì)胞因子IL-4、IL-6的表達(dá)量。結(jié)果表明,不同個(gè)體間免疫因子的表達(dá)率存在顯著差異;ConA和LPS刺激后差異不顯著。綜合分析個(gè)體間細(xì)胞免疫應(yīng)答能力,篩選免疫應(yīng)答較高與較低的個(gè)體。本研究采用牛支原體作為感染病原,對(duì)高免組和低免組的分組依據(jù)進(jìn)行驗(yàn)證。結(jié)果表明,支原體能夠干擾宿主的免疫應(yīng)答能力,對(duì)于免疫力較高的群體,支原體感染對(duì)宿主免疫應(yīng)答能力的干擾較弱,而對(duì)于免疫低的群體較強(qiáng)。
[Abstract]:The immune system is an important system for the body to carry out immune response and immune function. Some studies have found that the heritability of immune response is 25%. This heritability is similar to the heritability of production performance and somatotype. Making full use of the bull with high immune response ability can improve the utilization rate of bull and improve the whole immunity of cattle. And these higher immune responses can be inherited to the offspring. In this paper, the fine Holstein calf as the study object, cellular immunity and antibody immunization, flow cytometry, fluorescence quantitative PCR. Lymphocyte proliferation and ELISA liquid phase blocking antibody assay were used to analyze the immune response ability of different individuals. According to their immune response level, they were divided into high immune response individuals and low immune response individuals. The differences between individuals with high immune response ability and those with low immune response ability were analyzed. Humoral immunoassay was used to detect the serum antibody content of different individuals after immune stimulation by ELISA liquid phase blocking antibody. The fruit shows. There were significant differences in antibody content among different individuals. The antibody titers of one of the individuals with higher maternal antibody were significantly decreased after immune stimulation. High antibody mediated immune response individuals were screened according to the antibody titer. The higher antibody titers among the three subtypes of foot-and-mouth disease were compared with those with lower antibody titers. There were significant differences in the titer of type A antibody in different stages of immunostimulation. In the cellular immunity test, the expression rate of CD4 ~ CD8 ~ in T lymphocyte subsets was not significantly different before and after immunization. After ConA stimulation, there were significant differences in expression rates among different individuals. The results showed that the individuals with higher proliferation rate had higher immune response ability and those with lower proliferation rate had lower immune response ability. Fluorescence quantitative PCR test was used to detect the Th1 type IL-2 of T cell mitogen ConA and B cell mitogen LPS after stimulation. The expression of IFN- 緯 and Th2 cytokine IL-4 and IL-6. The results showed that there were significant differences in the expression rate of immune factors among different individuals. There was no significant difference between ConA and LPS after stimulation. The ability of cellular immune response between individuals was analyzed synthetically to screen the individuals with high and low immune response. Mycoplasma bovis was used as the pathogen of infection in this study. The results showed that mycoplasma could interfere with the immune response ability of the host. Mycoplasma infection interfered weakly with host immune response, but was stronger in low immune population.
【學(xué)位授予單位】：河北工程大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S823

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 邵南齊;孫永武;朱萱萱;高青;付金芳;王憲齡;;地魄湯對(duì)陰虛小鼠免疫功能的影響[J];河南中醫(yī);2016年10期

2 孫艷美;鐘秀宏;閆美田;黃郁雯;鄭楷;;樺褐孔菌多糖對(duì)環(huán)磷酰胺化療后小鼠免疫功能的研究[J];黑龍江畜牧獸醫(yī);2016年16期

3 牛暉;徐照學(xué);王二耀;馮亞杰;陳付英;張嘉保;;奶牛子宮內(nèi)膜炎血常規(guī)分析及病原分離鑒定[J];河南農(nóng)業(yè)科學(xué);2015年08期

4 林萍萍;李明春;;T淋巴細(xì)胞離子通道及其在自身免疫性疾病治療中的研究進(jìn)展[J];細(xì)胞與分子免疫學(xué)雜志;2015年05期

5 徐文俊;王莒生;;中藥對(duì)T淋巴細(xì)胞免疫抑制作用的研究進(jìn)展[J];世界中西醫(yī)結(jié)合雜志;2015年03期

6 李燕;徐慧芳;韓志剛;梁彩云;高凱;;CD_4~+T淋巴細(xì)胞增殖與艾滋病疾病進(jìn)展的關(guān)系研究[J];中國(guó)艾滋病性病;2013年02期

7 李志;楊婷婷;徐維家;;CD4~+T淋巴細(xì)胞與自身免疫性疾病的關(guān)系研究進(jìn)展[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2012年23期

8 肖進(jìn);王楠;巴利民;齊鵬;;牛外周血T淋巴細(xì)胞增殖試驗(yàn)條件的優(yōu)化[J];中國(guó)獸醫(yī)雜志;2012年11期

9 馬騰;劉巖;郝海生;杜衛(wèi)華;戴蘊(yùn)平;趙學(xué)明;王棟;秦彤;朱化彬;李發(fā)弟;王宗禮;;轉(zhuǎn)人α-乳清蛋白基因克隆牛及其胚胎移植受體血常規(guī)和血清生化指標(biāo)的研究[J];甘肅農(nóng)業(yè)大學(xué)學(xué)報(bào);2012年05期

10 吳俊;杜友衛(wèi);;血常規(guī)在寵物臨床上的意義[J];畜牧與飼料科學(xué);2011年11期

，

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