本文關(guān)鍵詞： SCD基因 鵝肥肝 miRNA 脂肪代謝　出處：《中國(guó)家禽》2017年02期 　論文類型：期刊論文

【摘要】：SCD1是位于內(nèi)質(zhì)網(wǎng)上的一類催化酶,在肝臟的脂質(zhì)代謝及鵝肥肝形成中發(fā)揮重要作用。試驗(yàn)通過(guò)熒光定量PCR技術(shù)對(duì)填飼不同階段(填飼7、14和19 d)朗德鵝肝臟中SCD1基因的表達(dá)進(jìn)行了研究。結(jié)果發(fā)現(xiàn):SCD1基因在填飼各階段肝臟中表達(dá)量均高于對(duì)照組,且填飼19 d時(shí),填飼組表達(dá)量極顯著高于對(duì)照組(P0.01);通過(guò)測(cè)定填飼組各時(shí)期肝臟中棕櫚油酸、油酸含量以及血液中甘油三酯和膽固醇含量發(fā)現(xiàn),SCD1基因表達(dá)量與油酸、膽固醇、VLDL以及甘油三酯含量均呈顯著正相關(guān)(P0.05);通過(guò)生物學(xué)軟件預(yù)測(cè)SCD1的靶向miRNA并利用雙熒光素酶報(bào)告系統(tǒng)在CHO細(xì)胞系中驗(yàn)證所篩選的3個(gè)miRNA,結(jié)果顯示miR30b與miR-30a-5p為鵝SCD1基因可能的靶向miRNA,進(jìn)一步研究發(fā)現(xiàn)miR-30b和miR-30a-5p在填飼組表達(dá)量均顯著低于對(duì)照組(P0.05),表明miR-30b和miR-30a-5p可能通過(guò)對(duì)SCD1基因的調(diào)節(jié)作用影響鵝肝臟脂代謝過(guò)程。
[Abstract]:SCD1 is a class of enzymes located in the endoplasmic reticulum, play an important role in hepatic lipid metabolism and the formation of goose liver. Test by fluorescence quantitative PCR in different stages of overfeeding (feeding 7,14 and 19 d) of SCD1 gene expression in the liver of Landes geese were studied. The results showed that the expression level of SCD1 gene were higher than the control group in each stage of feeding the liver, and feeding 19 D, feeding group was significantly higher than the control group (P0.01); the determination of fill in each period of liver fed group palmitoleic acid, oleic acid content and found in the blood triglyceride and cholesterol levels, SCD1 gene expression and oleic acid, cholesterol, there was significant positive correlation between VLDL and serum triglyceride were (P0.05); to miRNA and using the dual luciferase reporter system to verify 3 miRNA screening in CHO cell lines by biology software SCD1 forecast target, the results showed miR30b and miR-30a-5p For the goose SCD1 gene may target miRNA, further studies found that miR-30b and miR-30a-5p expression in feeding group was significantly lower than that in control group (P0.05), indicating that miR-30b and miR-30a-5p may affect the lipid metabolism process of foie gras by regulating SCD1 gene.

【作者單位】： 揚(yáng)州大學(xué)動(dòng)物科學(xué)與技術(shù)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31072021、31372298) 江蘇省普通高校研究生科研創(chuàng)新計(jì)劃項(xiàng)目(KYLX_1355)
【分類號(hào)】：S835
【正文快照】： 鵝肥肝是一種高級(jí)營(yíng)養(yǎng)食品,其味道鮮美,營(yíng)養(yǎng)豐富,能夠起到降低血脂、軟化血管、預(yù)防心血管疾病的作用[1]。目前,人們對(duì)于鵝肥肝形成的分子機(jī)理有了初步的認(rèn)識(shí)[2],但其深入的調(diào)控機(jī)制仍需要進(jìn)一步研究。硬脂酰輔酶A去飽和酶1(Stearoyl-Co A desaturase,SCD1)是一種內(nèi)質(zhì)網(wǎng)應(yīng)激酶

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