本文關(guān)鍵詞： PRRSV IL- PAMs p MAPK　出處：《中國動物傳染病學(xué)報》2017年02期 　論文類型：期刊論文

【摘要】：豬繁殖與呼吸綜合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)感染后能夠引發(fā)機體產(chǎn)生免疫抑制,IL-10在病毒介導(dǎo)的免疫抑制中起重要作用。PRRSV HuN4株感染PAM細(xì)胞后能夠誘導(dǎo)IL-10高水平表達(dá),而其傳代致弱毒株HuN4-F112感染PAM細(xì)胞后IL-10表達(dá)水平很低。本研究以PRRSV HuN4株及HuN4-F112株為研究對象,對其感染PAM細(xì)胞后IL-10上游關(guān)鍵因子表達(dá)差異進(jìn)行研究,揭示誘導(dǎo)IL-10產(chǎn)生差異的分子基礎(chǔ)。結(jié)果顯示,PRRSV強弱毒感染PAM細(xì)胞后,強毒HuN4株誘導(dǎo)TLR2與TLR4轉(zhuǎn)錄水平顯著高于弱毒HuN4-F112株,TLR3、TLR7和TLR9轉(zhuǎn)錄水平則無顯著差異;強毒HuN4株感染PAM細(xì)胞后,其p38磷酸化水平顯著高于弱毒HuN4-F112株,而ERK磷酸化水平差異不具備顯著統(tǒng)計學(xué)意義。結(jié)果提示,PRRSV強弱毒株感染PAM細(xì)胞誘導(dǎo)IL-10表達(dá)水平的差異推測是由于強弱毒誘導(dǎo)p38 MAPK磷酸化水平差異造成的。
[Abstract]:Porcine reproductive and respiratory syndrome virus. PRRSV-) infection can induce immune suppression in the body. IL-10 plays an important role in virus-mediated immunosuppression. PRRSv HuN4 strain can induce high IL-10 expression after infecting PAM cells. However, the expression of IL-10 was very low after the subculture of attenuated strain HuN4-F112 infected PAM cells. In this study, PRRSV HuN4 and HuN4-F112 strains were selected as the research objects. The differential expression of key factors in upstream of IL-10 was studied after its infection with PAM cells to reveal the molecular basis of inducing the difference in IL-10. After PRRSV was infected with PAM cells, the transcription levels of TLR2 and TLR4 induced by HuN4 strain were significantly higher than those of HuN4-F112 strain. There was no significant difference in transcription level between TLR7 and TLR9. The phosphorylation level of p38 in PAM cells infected with highly virulent HuN4 strain was significantly higher than that in attenuated HuN4-F112 strain, but there was no significant difference in ERK phosphorylation level. The difference of IL-10 expression induced by PAM cells infected with PRRSV strain is probably due to the difference of phosphorylation level of p38 MAPK induced by strong and weak virulence.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院上海獸醫(yī)研究所;江蘇高校動物重要疫病與人獸共患病防控協(xié)同創(chuàng)新中心;
【基金】：“十二五”農(nóng)村領(lǐng)域國家科技支撐計劃(2015BAD12B01) 國家自然科學(xué)基金面上項目(31670158)
【分類號】：S852.651
【正文快照】： 揚州225009)豬繁殖與呼吸綜合征病毒(Porcine reproductiveand respiratory syndrome virus,PRRSV)是有囊膜的單股正鏈RNA病毒[1]。PRRSV基因組含有10個開放閱讀框,可形成至少14種非結(jié)構(gòu)蛋白(Nsp1α、N s p 1β、N s p 1~N s p 6,N s p 7α,N s p 7β、Nsp8~Nsp12)和8種結(jié)構(gòu)蛋

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