本文關鍵詞： 水貂阿留申病 血清學調(diào)查 防制措施 膠體金試紙條　出處：《吉林農(nóng)業(yè)大學》2015年碩士論文　論文類型：學位論文

【摘要】：水貂阿留申病(AMD)是由水貂阿留申病毒(AMDV)引起的一種慢性傳染性疾病。為了進一步了解我國水貂阿留申病的流行情況,本研究在2013~2014年間,從我國黑龍江、河北、山東、遼寧和吉林5個主要的水貂養(yǎng)殖省份中收集了126748份樣品,采用CIEP法進行檢測,并對結果進行分析。黑龍江省共檢測了63799頭份,陽性率為29%;山東省檢測了23452頭份,陽性率為54%;河北省檢測了4570頭份,陽性率為44%;遼寧省檢測了26927頭份,陽性率為24%;吉林省檢測了8000頭份,陽性率為67%。表明黑龍江省和遼寧省的陽性率相對較低,吉林省,山東省和河北省陽性率較高,結果顯示整體陽性率在36%。本研究對黑龍江省帽兒山地區(qū)的部分水貂養(yǎng)殖場的患病率進行對比其中陽性率最低為13%,陽性率最高為85%。整體陽性率較低但部分養(yǎng)殖廠該疾病危害嚴重;隨后又對國內(nèi)五個采取防控措施的養(yǎng)殖場進行為期一年的跟蹤調(diào)查,發(fā)現(xiàn)陽性率下降了6%~25%,效果明顯;為進一步驗證水貂阿留申病對產(chǎn)仔率的影響,對某養(yǎng)殖場2013年和2014年的水貂患病率和群平均產(chǎn)仔數(shù)進行統(tǒng)計,得出水貂阿留申病陽性率下降了8%,貂群平均產(chǎn)仔成活數(shù)增加了0.55個,顯示水貂阿留申病影響產(chǎn)仔率。實驗中首次大批量應用水貂阿留申病膠體金試紙條與對流免疫電泳的結果進行對比,結果顯示兩種方法的符合率高達88%,且膠體金試紙條具有快速、易操作、靈敏等優(yōu)點,可成為未來阿留申病檢測的新方法。建議國內(nèi)消除AMD可利用膠體金試紙條和對流免疫電泳法相結合,采取養(yǎng)殖場層面淘汰阿留申病貂的策略,通過8~10年時間可將國內(nèi)AMD的陽性率控制在5%以下。本調(diào)查研究為國內(nèi)水貂阿留申病的預防和控制提供了參考和基礎數(shù)據(jù)。
[Abstract]:Mink Aleutian disease (AMDD) is a chronic infectious disease caused by mink Aleutian virus (AMDV). In order to further understand the prevalence of Aleutian disease in mink in China. In this study, 126748 samples were collected from 5 major mink breeding provinces in Heilongjiang, Hebei, Shandong, Liaoning and Jilin provinces in 2013 and 2014. The results were analyzed by CIEP method. A total of 63799 samples were detected in Heilongjiang Province, and the positive rate was 29. 23452 samples were tested in Shandong Province, and the positive rate was 54 pieces. 4570 samples were tested in Hebei Province, and the positive rate was 44. 26927 samples were tested in Liaoning Province, and the positive rate was 24. The positive rate of 8000 samples was detected in Jilin province, and the positive rate was 670.The results showed that the positive rate of Heilongjiang Province and Liaoning Province was relatively low, and the positive rate of Jilin Province, Shandong Province and Hebei Province was higher. The results showed that the overall positive rate was 36. This study compared the prevalence of some mink farms in Maoershan, Heilongjiang Province, among which the lowest positive rate was 13%. The highest positive rate was 85. The overall positive rate was lower but the disease was serious in some aquaculture plants. Then five domestic farms that take preventive and control measures were followed up for one year and the positive rate was found to have dropped by 6% and the effect was obvious. In order to further verify the effect of Aleutian disease on litter size, the prevalence of mink and the average litter size in a farm on 2013 and 2014 were analyzed. The results showed that the positive rate of Aleutian disease in mink decreased by 8%, and the average litter survival increased by 0.55. The results showed that Aleutian disease of mink had an effect on litter yield. In the experiment, the results of colloidal gold test strip of Aleutian disease of mink were compared with the results of convection immunoelectrophoresis. The results showed that the coincidence rate of the two methods was as high as 88%. The colloidal gold strip has the advantages of fast, easy to operate and sensitive, so it can be a new method for the detection of Aleutian disease in the future. It is suggested that the domestic elimination of AMD can be combined with colloidal gold test strip and convection immunoelectrophoresis. Adopt the strategy of eliminating Aleutian mink at farm level. The positive rate of AMD in China can be controlled below 5% in 8 ~ 10 years. This investigation provides reference and basic data for the prevention and control of Aleutian disease in mink.
【學位授予單位】：吉林農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.92

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本文編號：1446295