發(fā)布時(shí)間：2018-01-02 18:29

  本文關(guān)鍵詞：NELL1和FMO3基因在綿羊尾脂、皮下脂、腎周脂和肝臟中轉(zhuǎn)錄水平表達(dá)的研究　出處：《西北農(nóng)林科技大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 綿羊 NELL1 FMO3 表達(dá) mRNA

【摘要】：綿羊脂肪沉積的部位和沉積方式顯著影響羊肉品質(zhì)和養(yǎng)羊效益。因此,揭示功能基因、日糧及生長(zhǎng)階段對(duì)綿羊脂肪沉積的影響,對(duì)改良綿羊肉品質(zhì)具有重要意義。研究選取了脂肪代謝相關(guān)功能基因NELL1和FMO3,比較其在不同尾型綿羊品種(哈薩克羊、灘羊、同羊、西藏羊)尾部脂肪組織中的表達(dá)差異;探討營養(yǎng)水平及生長(zhǎng)階段對(duì)其在灘羊脂肪組織中表達(dá)的影響,旨在揭示不同營養(yǎng)水平及不同生長(zhǎng)階段脂尾型綿羊NELL1和FMO3基因表達(dá)的組織特異性及其表達(dá)模式,為脂尾型綿羊體內(nèi)脂肪沉積的營養(yǎng)調(diào)控提供理論依據(jù)。試驗(yàn)一:不同品種綿羊尾脂中NELL1和FMO3 mRNA表達(dá)差異分析選擇健康、體重相近的9月齡灘羊、同羊、哈薩克羊和西藏羊羯羊各3只,采集尾部脂肪組織,利用實(shí)時(shí)熒光定量PCR技術(shù),檢測(cè)NELL1和FMO3 mRNA表達(dá),結(jié)果如下:1.FMO3mRNA在哈薩克羊尾脂中表達(dá)量最高(P0.05);在同羊、灘羊、西藏羊尾脂中依次下降,且差異顯著(P0.05)。2.NELL1mRNA在西藏羊尾脂中表達(dá)量最高(P0.05);在灘羊、同羊、哈薩克羊中表達(dá)量依次下降。試驗(yàn)二:營養(yǎng)水平對(duì)不同階段灘羊部分組織中NELL1和FMO3 mRNA表達(dá)的影響選擇健康4月齡灘羊112只隨機(jī)分為4組,公母各半,每組四個(gè)重復(fù),每個(gè)重復(fù)7只羊。試驗(yàn)根據(jù)增重目標(biāo)分為3個(gè)階段:第一階段(22~28kg),第二階段(28~35kg),第三階段(35~40kg),每個(gè)階段各組分別飼喂不同營養(yǎng)水平的日糧,由低到高分為T1、T2、T3、T4組。每個(gè)生長(zhǎng)階段結(jié)束時(shí)采集肝臟、腎周脂肪、尾脂、皮下脂肪的組織樣品,提取樣品總RNA,采用實(shí)時(shí)熒光定量PCR的方法分析NELL1和FMO3 mRNA的表達(dá)差異。結(jié)果如下:1.NELL1 mRNA在尾脂中的表達(dá),第1階段以T4組為最高(P0.05),其他三組差異不顯著;第2階段和第3階段,也都是以T4組表達(dá)量最高(P0.05),T1、T2、T3組表達(dá)量略有波動(dòng)。2.NELL1 mRNA在皮下脂中的表達(dá)。第1階段和第2階段四個(gè)營養(yǎng)水平組間表達(dá)差異不顯著(P0.05);第3階段,T4組表達(dá)量最高(P0.05),依次為T2、T3、T1組。相同營養(yǎng)水平下,NELL1 mRNA在第1階段表達(dá)量最高(P0.05)。3.NELL1 mRNA在腎周脂中的表達(dá)。在同一生長(zhǎng)階段,營養(yǎng)水平對(duì)灘羊腎周脂肪組織中NELL1mRNA的表達(dá)無顯著影響(P0.05);在相同營養(yǎng)水平下,不同生長(zhǎng)階段間表現(xiàn)出表達(dá)差異顯著(P0.05)。4.NELLL1 mRNA在肝臟中的表達(dá)。第1階段,在四個(gè)營養(yǎng)水平組間表達(dá)差異不顯著;第2階段,表達(dá)以T4組最高(P0.05),依次為T3、T1、T2組;第3階段,表達(dá)以T1組為最高(P0.05),依次為T2、T3、T4組。3個(gè)階段表達(dá)依次升高,其中第3階段表達(dá)量最高(P0.05)。5.FMO3 mRNA的表達(dá)。尾部脂肪組織中最高表達(dá)均出現(xiàn)在T2組;在第1階段、第2階段和第3階段,T1、T2、T3、T4組中表達(dá)量均呈現(xiàn)先升高再降低的趨勢(shì);皮下脂肪組織T3組最高,腎周脂最高表達(dá)出現(xiàn)在T1組,肝臟的最高表達(dá)出現(xiàn)在第T4組,最高表達(dá)組與相同階段時(shí)的最低表達(dá)組相比差異顯著的(P0.05)。試驗(yàn)三:NELL1、FMO3mRNA在灘羊不同組織中的表達(dá)差異分析在試驗(yàn)第三階段結(jié)束當(dāng)天,選取T2組4只灘羊進(jìn)行屠宰,采集肝臟、腎周脂肪、尾脂、皮下脂肪組織,利用實(shí)時(shí)熒光定量PCR技術(shù),檢測(cè)NELL1、FMO3mRNA的相對(duì)表達(dá)量,結(jié)果如下:1.NELL1 mRNA在灘羊尾部脂肪組織、皮下脂肪組織、腎周脂肪組織、肝臟中均有表達(dá)。NELL1在肝臟組織中表達(dá)最高,顯著高于尾脂、皮下脂和腎周脂(P0.05),其次是在腎周脂中的表達(dá),高于尾脂和皮下脂(P0.05),在尾脂和皮下脂中的表達(dá)最低,兩者之間差異不顯(P0.05)。2.FMO3 mRNA在灘羊尾部脂肪組織、皮下脂肪組織、腎周脂肪組織、肝臟中均有表達(dá)。FMO3在肝臟組織中表達(dá)最高,顯著高于尾部脂肪組織、皮下脂肪組織和腎周脂肪組織(P0.05),其次是在尾部脂肪組織中的表達(dá),高于腎周脂肪組織和皮下脂肪組織(P0.05),在腎周脂肪組織和皮下脂肪組織中的表達(dá)最低,兩者之間差異不顯(P0.05)。綜合以上試驗(yàn)結(jié)果,表明NELL1和FMO3基因在綿羊組織中的表達(dá)受到品種、部位、生長(zhǎng)階段、營養(yǎng)水平等原因會(huì)有不同的表達(dá)結(jié)果,其表達(dá)差異是與脂肪的沉積差異是存在一定關(guān)系。
[Abstract]:Location and mode of deposition of sheep fat deposition significantly affect the quality of mutton and sheep benefits. Therefore, to reveal the functional gene, the effects of dietary and growth stages of sheep fat deposition, and has the important significance for improving the quality of sheep meat. The fat metabolism related genes NELL1 and FMO3, the tail type in different sheep breeds (Kazak sheep, Tan sheep, Tong sheep, Tibet sheep) differentially expressed in adipose tissue of the tail; nutrition level and growth effects on its expression in sheep adipose tissue, to reveal the different nutritional levels and different growth stages of fat tail sheep NELL1 and expression of FMO3 gene and tissue specific expression pattern. To provide a theoretical basis for the nutritional regulation of fat deposition of fat tail sheep. Experiment one: compare health NELL1 and the expression of mRNA FMO3 in different varieties of sheep tail fat, body weight were 9 Month old Tan sheep, Tong sheep, Kazak sheep and Tibet sheep were all 3, collected tail fat tissue, using real-time fluorescence quantitative PCR technology, detection of NELL1 and FMO3 mRNA expression, the results are as follows: 1.FMO3mRNA in Kazak sheep tail fat expression was the highest (P0.05); in the same Tan sheep, Tibet sheep tail fat. In order to decrease, and the difference was significant (P0.05).2.NELL1mRNA in Tibet sheep tail fat the highest expression level (P0.05); in the Tan sheep, Tong sheep, Kazakstan sheep in the amount of expression gradually decreased. Experiment two: the influence of nutrition level on the expression of NELL1 and FMO3 mRNA part of the organization in different stages of selection in the April health sheep old Tan sheep were randomly 112 divided into 4 groups, male and female, with four replicates, each replicate 7 sheep. The test is divided into 3 stages according to the weight target: the first stage (22~28kg), the second stage (28~35kg), the third stage (35~40kg), each stage of the four groups were fed with different nutritional levels of the day Grain, from low to high as T1, T2, T3, T4 group. The end of each growth stage when collecting the liver, perirenal fat, fat tail, subcutaneous adipose tissue samples were extracted total RNA expression by real-time quantitative PCR analysis of NELL1 and FMO3 mRNA. The results are as follows: the expression of 1.NELL1 mRNA in the first stage of fat tail, T4 group was the highest (P0.05), the other three groups had no significant difference; the second and third stages, is also the highest expression level in T4 group (P0.05), T1, T2, T3 group, the expression of.2.NELL1 mRNA in slight fluctuations in subcutaneous fat in first stages. And the second stage four nutrition level group. The expression difference was not significant (P0.05); the third stage, the highest expression level in T4 group (P0.05), followed by T2, T3, T1 group. The same nutrient level, NELL1 mRNA expression was the highest in the first stage (P0.05) expression of.3.NELL1 mRNA in kidney at the same Zhou Zhizhong. A period of growth, nutrition level of Tan sheep No significant effect on the expression of NELL1mRNA in perirenal adipose tissue (P0.05); in the same nutrient level, different growth stages showed significant differences in the expression of.4.NELLL1 mRNA (P0.05) expression in the liver. In the first stage, in the four trophic level group. The expression difference was not significant; the second stage, the expression of T4 was the highest (P0.05), followed by T3, T1, T2 group; the third stage, the expression in T1 group was the highest (P0.05), followed by T2, T3, T4 group.3 expression gradually increased, the third stage of the highest expression level (P0.05). The expression of.5.FMO3 mRNA were found in T2 group is the highest expression of fat tail fat tissue; in the first stage, second stage and third stage, T1, T2, T3, T4 expression in the group volume showed first increased and then decreased; subcutaneous adipose tissue of T3 group was the highest, perirenal fat was the highest in T1 group, the highest expression in the liver of T4 group, the highest expression group with the same stage of the lowest The expression was significantly different (P0.05). Experiment three: NELL1 analysis of expression of FMO3mRNA in different tissues of sheep in the third trial of the end of the day, T2 group selected 4 Tan sheep were slaughtered, collected liver, perirenal fat, fat tail, subcutaneous adipose tissue, detection of NELL1 using real-time quantitative PCR technique and the relative expression of FMO3mRNA, the results are as follows: 1.NELL1 mRNA in sheep tail fat, subcutaneous adipose tissue, perirenal adipose tissue, the expression of.NELL1 had the highest expression in the liver tissue in the liver, was significantly higher than that of tail fat, subcutaneous fat and perirenal fat (P0.05), followed by the expression of perirenal Zhizhong, higher than tail fat and subcutaneous fat (P0.05), the lowest expression in tail fat and subcutaneous fat in between the two, the difference was not significant (P0.05).2.FMO3 mRNA in sheep tail fat, subcutaneous adipose tissue, perirenal adipose tissue,.FMO3 was expressed in the liver in the liver The highest expression was significantly higher than that of the tail, adipose tissue, subcutaneous adipose tissue and perirenal adipose tissue (P0.05), the second is expressed in tail fat tissue, higher in perirenal adipose tissue and subcutaneous adipose tissue (P0.05), in perirenal adipose tissue and subcutaneous adipose tissue was the lowest, the difference between the two no significant (P0.05). These test results showed that the expression of NELL1 and FMO3 gene in sheep tissues by species, site, growth stage, nutrient levels and other reasons will result expression of different, its expression difference is and there are differences in fat deposition.

【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S826

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1 田崇奇;NELL1和FMO3基因在綿羊尾脂、皮下脂、腎周脂和肝臟中轉(zhuǎn)錄水平表達(dá)的研究[D];西北農(nóng)林科技大學(xué);2016年

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本文編號(hào)：1370417