ZHX2（zinc fingers and homoboxes 2）,即鋅指結(jié)構(gòu)與同源結(jié)構(gòu)域蛋白2,是一個(gè)轉(zhuǎn)錄抑制因子,與ZHX1、ZHX3組成鋅指結(jié)構(gòu)及同源結(jié)構(gòu)域家族蛋白。研究發(fā)現(xiàn),ZHX2在肝癌、肺癌、霍奇金淋巴瘤等多種惡性腫瘤疾病中表達(dá)下調(diào),因此ZHX2被鑒定為一個(gè)新的抑癌基因。此外,ZHX2尚可轉(zhuǎn)錄抑制肝癌細(xì)胞中重要的腫瘤標(biāo)記物甲胎蛋白（AFP）、GPC3的表達(dá),并通過(guò)調(diào)控細(xì)胞周期蛋白,抑制細(xì)胞的過(guò)度增殖,進(jìn)一步說(shuō)明ZHX2在抑制腫瘤發(fā)生和進(jìn)程中起著重要作用,并為肝癌的診斷和預(yù)后監(jiān)測(cè)提供了新的候選分子。與其他遺傳背景相似的小鼠相比,Balb/cJ小鼠肝臟內(nèi)的ZHX2表達(dá)水平顯著降低,并可以避免高脂飲食飼喂誘導(dǎo)的高脂血癥。通過(guò)數(shù)量性狀位點(diǎn)圖譜鑒定發(fā)現(xiàn),脂質(zhì)代謝相關(guān)基因LPL的表達(dá)水平與ZHX2表達(dá)水平呈現(xiàn)相反的表達(dá)趨勢(shì),這也許是因?yàn)轱曃垢咧嬍澈?Balb/cJ小鼠肝臟中高表達(dá)的LPL能攝取更多血漿甘油三酯,使血漿中甘油三酯的水平低于其他品系的小鼠。LPL全稱(chēng)脂蛋白脂肪酶,是機(jī)體分解甘油三酯的關(guān)鍵酶。LPL通過(guò)肝素結(jié)合位點(diǎn)以同源二聚體的形式錨定在血管內(nèi)皮細(xì)胞表面,負(fù)責(zé)攝取載脂蛋白... 

【文章來(lái)源】：山東大學(xué)山東省 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：72 頁(yè)

【學(xué)位級(jí)別】：碩士

【部分圖文】：

圖２．肝癌細(xì)胞系中ＺＨＸ２?（２４２－４４６?ａａ）是抑制ＬＰＬ表達(dá)的較小功能區(qū)??Ｆｉｇｕｒｅ２?ＺＨＸ２?（２４２－４４６ａａ）?ｉｓ?ｔｈｅ?ｆｕｎｃｔｉｏｎ?ｄｏｍａｉｎ?ｆｏｒ??ｉｎｈｉｂｉｔｉｎｇ?ＬＰＬ?ｅｘｐｒｅ巧ｉｏｎ?ｉｎ?ｈｅｐａｔｏｍａ?ｃｅｌｌ?ｌｉｎｅｓ??．－

圖３．?ＺＨＸ２?（２４２－４４６ａａ）抑制Ｌ化核也啟動(dòng)子活性??Ｆｉｇｕｒｅ?３?ＺＨＸ２?（２４２－４４６ａａ）?ｉｎｈｉｂｉｔｓ?ＬＰＬ?ｃｏｒｅ?ｐｒｏｍｏｔｅｒ?ａｃｔｉｖｉｔｙ??

圖４．ＣｏｎＡ誘導(dǎo)肝炎模型后肝臟ＺＨＸ２表達(dá)上調(diào)??Ｆｉｇｕｒｅ４?之ＨＸ２?ｉｓ?ｕｐ－ｒｅｇｕｌａｔｅｄ?ｉｎ?化ｅ?ｍｏｕｓｅ?ｌｉｖｅｒ?ｏｆ?ＣｏｎＡ?ｉｎｄｕｃｅｄ?ｈｅｐａｔｉｔｉｓ??１．?２?ＬＰＳ／Ｇａ?ＩＮ肝炎模型肝組織中的ＺＨＸ２表達(dá)上巧??

【參考文獻(xiàn)】：
期刊論文
[1]Immune mechanisms of Concanavalin A model of autoimmune hepatitis[J]. Hai-Xia Wang,College of Sciences,Anhui Science and Technology University,Fengyang 233100,Anhui Province,China Man Liu,Shun-Yan Weng,Jing-Jing Li,Chao Xie,Hong-Lin He,Wen Guan,Yun-Sheng Yuan,Shanghai Municipality Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China Jin Gao,Laboratory of Regeneration,School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China.  World Journal of Gastroenterology. 2012(02)
[2]Kupffer cells contribute to concanavalin A-induced hepatic injury through a Th1 but not Th17 type response-dependent pathway in mice[J]. Lin Chen,Xiao-Jun Xie,Yu-Fu Ye,Lin Zhou,Hai-Yang Xie,Qin-Fen Xie, Jiong Tian and Shu-Sen ZhengZhejiang University School of Medicine,Hangzhou 310003,ChinaKey Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health;Department of Hepatobiliary and Pancreatic Surgery Department of Nephrology,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China.  Hepatobiliary & Pancreatic Diseases International. 2011(02)



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