血管緊張素Ⅱ的生長調(diào)節(jié)作用 概述 腎素-血管緊張素系統(tǒng)傳統(tǒng)上被歸類為系統(tǒng)性激素調(diào)節(jié)系統(tǒng)。它的主要活性物質(zhì)是血管緊張素Ⅱ（AⅡ）。血管緊張素Ⅱ來源于血漿α₂-球旦白，即血管緊張素原，它的形成和釋放過程簡述如下： 血管緊張素原 ↓腎素 血管緊張素Ⅰ ↓轉(zhuǎn)化酶 血管緊張素Ⅱ（AⅡ） AⅡ具有很強(qiáng)的血管收縮作用及促進(jìn)醛固酮分泌的作用，其結(jié)果主要是導(dǎo)致血壓升高和鈉鹽儲(chǔ)留。所以腎素-血管緊張素系統(tǒng)一直被認(rèn)為是一種作用于心血管的內(nèi)分泌系統(tǒng)。然而，近年來發(fā)現(xiàn)腎素-血管緊張素系統(tǒng)不僅分布在心臟，腎臟及腦組織，而且存在于卵巢和脂肪組織。內(nèi)分泌激素局部作用的發(fā)現(xiàn)更促使人們重新探討腎素-血管緊張素的其它作用。近幾十年來，已有許多實(shí)驗(yàn)室相繼報(bào)道AⅡ可以產(chǎn)生于局部，以旁分泌或自分泌的方式調(diào)節(jié)局部組織細(xì)胞的生長繁殖。 高血壓導(dǎo)致的心肌肥大一貫被認(rèn)為是繼發(fā)于血流動(dòng)力學(xué)變化的代償性肥大。但已有證據(jù)表明心室肥大的程度與血壓升高的程度沒有密切的相關(guān)性。例如自發(fā)性高血壓大鼠的心肌肥大發(fā)生在血壓明顯升高之前；臨床觀察發(fā)現(xiàn)血壓很高的病人并不一定發(fā)生明顯的... 

【文章來源】：北京協(xié)和醫(yī)學(xué)院北京市 211工程院校 985工程院校

【文章頁數(shù)】：76 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
中文摘要
INTRODUCTION
1. The tissue renin-angiotensin systems（RAS）
2. Cardiac hypertrophy in hypertension
    a. hemodynamic
    b. pharmacology
    c. hemadynamic factors may at least partially affect cardiac mass through intrinsic renin-angiotensin system
3. Angiotensin receptors and signalling system
4. Renin-angiotensin system in neuroblastoma cells
5. N-myc expression in neuroblastoma
6. Molecular biology of angiotensinogen
7. Role of AII in gene transcription
8. Regulation of angiotensinogen gene expression
EXPERIMENTS
    EXPERIMENT 1: Regulation of ANGT expression the hearts of hypertensive rats
        1. Animals
        2. Northern blot and slot blot analysis
            Isolation of Total RNA Utilizing the Guanidine Isothiocyanate Method
            Electrophoresis of RNA
            Nick Translation Procedure
            Purification Procedure of labeled probe
            Hybridization And Washing Procedure
        RESULTS
    EXPERIMENT 2: Effects of converting enzyme inhibitors on the growth of neuroblastoma cell line SHSY5Y
        Materials and Methods
        Results
    EXPERIMENT 3, The interaction of insulin and angiotensin II on the regulation of human neuroblastoma cell growth
        MATERIALS AND METHODS
        RESULTS
    EXPERIMENT 4: Effect enalapril and DUP 753 on N-myc mRNA expression in SHSY5Y cells
        Material and methods
        RESULTS
125I All binding assay onSHSY5Y cell">    EXPERIMENT 5: ¹²⁵I All binding assay onSHSY5Y cell
        Materials and Methods
        RESULTS
    EXPERIMENT 6: Cloning of ANGT promotor DNA and nuclear protein binding assay
        First goal: obtaining ANGT promoter DNA
        Second goal: utilizing the promoter DNA to study on the gene regulation
        RESULTS
DISCUSSION
    conclusion
References



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