發(fā)布時(shí)間：2019-07-01 18:24

【摘要】：目的采用實(shí)時(shí)熒光PCR法、酶聯(lián)免疫吸附法及細(xì)胞培養(yǎng)分離病毒株的方法,對(duì)2013年臨床疑似發(fā)熱伴血小板減少綜合征患者急性期血進(jìn)行檢測(cè)比較,尋求快速、準(zhǔn)確的鑒定新布尼亞病毒感染的方法。方法對(duì)180例臨床疑似病例的急性期血液首先采用實(shí)時(shí)熒光PCR法檢測(cè),篩選出新布尼亞病毒核酸陽性的樣本,同時(shí)將陽性樣本采用酶聯(lián)免疫吸附的方法檢測(cè)抗體及采用VERO-E6細(xì)胞培養(yǎng)的方法分離病毒株。結(jié)果 180份樣本經(jīng)實(shí)時(shí)熒光PCR法檢測(cè),陽性率為42.78%(77/180Ct值≤35曲線形態(tài)與陽性對(duì)照一致呈S型的),ELISA方法檢測(cè)抗體陽性率為44.16%(34/77),將核酸檢測(cè)陽性的77份樣本全部感染VERO-E6細(xì)胞獲得病毒株32株,陽性率為38.55%(32/77)。結(jié)論實(shí)時(shí)熒光PCR法更適合于新布尼亞病毒感染者早期實(shí)驗(yàn)室快速診斷,發(fā)病在1w左右的病例不適合用ELISA的方法做確證實(shí)驗(yàn)。細(xì)胞培養(yǎng)分離病毒雖然是金標(biāo)準(zhǔn),但耗時(shí)、費(fèi)力,對(duì)早期診斷意義不大,但適于研究或疫苗研發(fā)。
[Abstract]:Objective to detect and compare the acute phase blood of suspected febrile patients with thrombopenia syndrome in 2013 by real-time fluorescence PCR, enzyme-linked immunosorbent assay (Elisa) and cell culture, and to find a rapid and accurate method for the identification of new Bunia virus infection. Methods the blood samples of 180 suspected clinical cases in acute phase were detected by real-time fluorescence PCR, and the positive samples were screened out. At the same time, the positive samples were detected by enzyme-linked immunosorbent assay (Elisa) and the virus strains were isolated by VERO-E6 cell culture. Results the positive rate of antibody was 42.78% by real-time fluorescence PCR (77/180Ct 鈮，

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