高溫致畸金黃地鼠胚胎神經(jīng)管cDNA文庫(kù)的構(gòu)建及致畸相關(guān)基因的篩查
[Abstract]:In the process of vertebrate embryo development, neuroembryo is an important developmental period. At this stage, the ectoderm thickens to form an epithelial nerve plate, which forms a nerve fold on both sides of the nerve plate, and then the two sides of the nerve fold approach the dorsal midline and heal into a neural tube. The occurrence and differentiation of neural tube is the premise of normal development of brain and spinal cord, which will lead to neural tube malformation (neural tube defects,NTDs). With the development of modern industry and the increasingly serious environmental pollution, the incidence of congenital malformations in human beings is also increasing year by year. The results of 1986 survey show that the incidence of congenital malformations in China is as high as 1.3%. Therefore, more and more attention has been paid to the study of congenital malformations. Among all kinds of congenital malformations, neural tube malformation is the highest incidence and the most serious congenital malformation, which is caused by incomplete or delayed closure of neural tube in the early stage of embryonic development, mainly involving the central nervous system, including anencephalia, open brain, spina bifida and so on. Neural tube malformation is also one of the main causes of stillbirth, abortion and premature delivery, and its disability rate is very high, which brings serious psychological and economic burden to family and society. The etiology of NTDs is very complex, there are both genetic and environmental factors, but also contains the interaction between genetic factors and environmental factors. Epidemiological investigation and animal experiments show that high temperature is the most common and difficult to prevent physical teratogenic factors, which can cause congenital malformations in many animals and humans, among which NTDs has the highest occurrence rate. In recent years, scholars from all over the world have done a lot of animal experiments and cell culture studies on high temperature teratogenesis. The effects of high temperature on cell proliferation, apoptosis, cell differentiation, cell adhesion, cell clustering, tissue induction and organogenesis have been observed, but the molecular mechanism of high temperature teratogenesis has not yet been clarified. NTDs induced by high temperature is an extremely complex process, in which some proteins are synthesized, especially with embryonic differentiation.
【學(xué)位授予單位】:山東大學(xué)
【學(xué)位級(jí)別】:博士
【學(xué)位授予年份】:2006
【分類號(hào)】:R321.6
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