【摘要】： 目的：觀察比較孕鼠著床期子宮內(nèi)膜中免疫調(diào)節(jié)蛋白孕酮誘導(dǎo)的封閉因子(progesterone-induced blocking factor，PIBF)、雌激素受體(estrogen receptor-a，ER-a)、孕激素受體(progesterone receptor，PR)的表達以及血清雌二醇(estradiol，E_2)及孕酮(progesterone，P)含量差異，探討外源性孕激素補充對胚胎著床期免疫的影響及其影響途徑。 方法：昆明小鼠隨機分為地屈孕酮組(從孕1天起每日灌胃2mg地屈孕酮)、黃體酮組(從孕1天起頸背部皮下注射2mg黃體酮)和對照組，分別于孕4、5、6天收集子宮及血清；C57BL／6雌鼠隨機分為同基因交配組(與C57BL／6雄鼠合籠交配)和異基因組(與BALB／c雄鼠合籠交配)，在孕6天收集子宮及血清。免疫組化法檢測各組子宮內(nèi)膜PIBF、ER-a、PR表達，放射免疫法檢測血清E_2及P含量，對比分析各組間差異。 結(jié)果：(1)異基因交配組(C57BL／6×BALB／c)較同基因交配組(C57BL／6×C57BL／6)子宮內(nèi)膜PIBF表達明顯增高(p＜0.05)，血清E_2、P含量和子宮內(nèi)膜ER-a、PR表達均顯著升高(p＜0.05)。(2)外源性孕激素補充(包括天然黃體酮和地屈孕酮)的孕鼠其子宮內(nèi)膜PIBF表達明顯增高(p＜0.05)，地屈孕酮組較黃體酮組PIBF表達更高，差異有顯著性(p＜0.05)。黃體酮組血清P含量顯著升高(p＜0.01)，ER-a和PR表達下降(p＜0.05)。地屈孕酮組血清E_2、P含量無變化(p＞0.05)，但子宮內(nèi)膜ER-a、PR表達顯著升高(p＜0.05)。 結(jié)論：PIBF的表達需要異基因胚胎抗原的刺激；地屈孕酮和黃體酮均上調(diào)PIBF表達，，地屈孕酮的作用強于黃體酮，且前者上調(diào)而后者下調(diào)子宮內(nèi)膜ER-a、PR的表達。
[Abstract]:Objective: to observe and compare the blocking factor (progesterone-induced blocking factor,PIBF), estrogen receptor (estrogen receptor-a,ER-a) and progesterone receptor (progesterone receptor, induced by progesterone in the uterus of pregnant rats during implantation. The expression of PR) and the difference of serum estrogen (estradiol,E_2) and progesterone (progesterone,P) levels were studied to explore the effect of exogenous progesterone supplementation on embryo implantation immunity. Methods: Kunming mice were randomly divided into diethylprogesterone group (2mg diprogesterone group), progesterone group (subcutaneous injection of 2mg progesterone into the neck and back from the 1st day of pregnancy) and control group. Uterus and serum were collected on the 4th and 5th day of gestation, respectively. the uterus and serum were collected on the 6th day of gestation, respectively. the uterus and serum were collected on the 4th and 5th day of gestation. C57BL/6 female mice were randomly divided into homogenic mating group (mated with C57BL/6 male mice) and heterogenomes (mated with BALB/c male mice). Uterus and serum were collected on the 6th day of pregnancy. The expression of PIBF,ER-a,PR in uterus was detected by immunohistochemistry, and the contents of E 鈮

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