【摘要】：旋毛蟲病是一種嚴(yán)重的人獸共患寄生蟲病，主要因生食或半生食含有旋毛蟲幼蟲囊包的豬肉或其它動(dòng)物肉類而感染。因此，對(duì)肉類及肉制品中進(jìn)行旋毛蟲檢疫是旋毛蟲病防治工作的重要內(nèi)容。目前用于肉類中旋毛蟲檢疫的方法有旋毛蟲鏡檢查法和人工消化法，但前者的敏感性低，而后者操作較復(fù)雜不易推廣應(yīng)用。由于IgG抗體在宿主血液和組織液內(nèi)均有分布，應(yīng)用免疫學(xué)方法從動(dòng)物肉汁中進(jìn)行抗旋毛蟲抗體的檢測(cè)有望用于旋毛蟲的檢疫。然而，關(guān)于動(dòng)物感染旋毛蟲后肉汁中抗體水平的動(dòng)態(tài)變化尚未見報(bào)道。鑒于此，本文應(yīng)用旋毛蟲小鼠動(dòng)物模型，通過(guò)間接ELISA對(duì)不同劑量旋毛蟲感染小鼠后不同時(shí)間血清和肉汁的抗體水平進(jìn)行了研究；并對(duì)小鼠肌肉在不同溫度下保存不同時(shí)間后肉汁抗體水平的動(dòng)態(tài)變化進(jìn)行了觀察。 材料和方法 1．對(duì)ELISA檢測(cè)旋毛蟲感染小鼠肉汁抗體的最適條件進(jìn)行了選擇，包括抗原的最適包被濃度及血清最佳稀釋度，酶標(biāo)二抗、肉汁最佳稀釋液及最佳稀釋度的選擇。 2．將300只健康昆明小鼠隨機(jī)分成3組，每組100只。每組分別感染旋毛蟲100條／只(輕度感染組)、300條／只(中度感染組)和500條／只(重度感染組)，觀察小鼠感染不同劑量旋毛蟲后不同時(shí)間(1-18周)血清及肉汁抗體水平的變化。 3．將32只健康昆明小鼠隨機(jī)分為4組，每組8只。每只分別感染50、100、300、500條旋毛蟲，感染6后周觀察小鼠膈肌蟲荷與血清及肉汁抗體水平的關(guān)系。 4．將70只健康昆明小鼠隨機(jī)分成5組，每組14只。5組分別感染不同種的旋毛蟲(旋毛蟲、鄉(xiāng)土旋毛蟲、布氏旋毛蟲、偽旋毛蟲、納氏旋毛蟲)，，每只感染500條幼蟲。感染后
[Abstract]:Trichinellosis is a serious zoonotic parasitic disease which is mainly infected by raw or half-eaten pork or other animal meat containing the cyst of Trichinella spiralis larvae. Therefore, the quarantine of Trichinella spiralis in meat and meat products is an important part of trichinellosis control. At present, the quarantine methods of Trichinella spiralis in meat are Trichinella spiralis microscopic examination and artificial digestion, but the former has low sensitivity, and the latter is difficult to be popularized and applied in complicated operation. Because of the distribution of IgG antibody in host blood and tissue fluid, the detection of anti-Trichinella antibody from animal gravy by immunology is expected to be used for quarantine of Trichinella spiralis. However, the dynamic changes of antibody levels in succulent juice of animals infected with Trichinella spiralis have not been reported. In view of this, an animal model of Trichinella spiralis (Trichinella spiralis) was used to study the antibody levels in serum and gravy of mice infected with Trichinella spiralis (Trichinella spiralis) at different time points by indirect ELISA. The dynamic changes of antibody level of succulent were observed after the muscle was stored at different temperatures for different time. Materials and methods 1. The optimum conditions for detection of antibody against Trichinella spiralis infection in mouse gravy by ELISA were selected, including the optimum coating concentration of antigen and the best dilution of serum, enzyme-labeled secondary antibody, The best diluent and the best dilution of gravy. 2. 300 healthy Kunming mice were randomly divided into 3 groups with 100 mice in each group. There were 100 Trichinella spiralis in each group (mild infection group), 300 Trichinella spiralis (moderate infection group) and 500 Trichinella spiralis (severe infection group). The changes of antibody levels in serum and gravy of mice infected with different doses of Trichinella spiralis were observed at different time (1-18 weeks). 3. 32 healthy Kunming mice were randomly divided into 4 groups with 8 mice in each group. Each Trichinella spiralis was infected with 50100300500 Trichinella spiralis respectively. Six weeks after infection, the relationship between phrenic myomyotic charge and antibody levels in serum and gravy in mice was observed. 4. Seventy healthy Kunming mice were randomly divided into 5 groups with 14 mice in each group. Five groups were infected with different species of Trichinella spiralis (Trichinella spiralis, Trichinella bruciformis, Trichinella pseudotinalis, Trichinella nahrensis), each infected with 500 larvae. After infection
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 申麗潔,羅志勇,李偉;感染旋毛蟲小鼠外周血有核細(xì)胞及抗體水平的動(dòng)態(tài)觀察[J];大理醫(yī)學(xué)院學(xué)報(bào);2000年02期

2 崔晶;旋毛蟲幼蟲囊包的結(jié)構(gòu)及其形成機(jī)制[J];國(guó)外醫(yī)學(xué)(寄生蟲病分冊(cè));2002年05期

3 陳春花,李喜仙,閻玉河,鄧瑞廣;旋毛蟲基因重組抗原快速ELISA診斷試劑盒的研究[J];華北農(nóng)學(xué)報(bào);2002年02期

4 羅公平,林明亮,何浩,張彥慶,姜淑珍,胡天陽(yáng),胡艷君,張?jiān)瀑F,盧學(xué)利,石金峰,宋銘忻,路義鑫;不同旋毛蟲隔離種感染豬檢疫情況及其分類研究[J];黑龍江畜牧獸醫(yī);1999年07期

5 韓化敏,崔晶,王中全,衛(wèi)海燕,晉雪香;旋毛蟲Ts43基因克隆和序列分析[J];鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年04期

6 崔晶，王中全，晉雪香，武峰，毛福榮;酶聯(lián)免疫吸附試驗(yàn)對(duì)人體旋毛蟲病診斷及療效考核價(jià)值的研究[J];河南醫(yī)學(xué)研究;1996年01期

7 張建偉;ELISA法中出現(xiàn)非特異性顯色原因分析[J];臨床輸血與檢驗(yàn);2005年01期

8 劉明遠(yuǎn);;我國(guó)旋毛蟲病流行病學(xué)概況與研究前瞻[J];中國(guó)農(nóng)業(yè)大學(xué)學(xué)報(bào);1998年S2期

9 朱本志;豬旋毛蟲的檢驗(yàn)及處理[J];肉類工業(yè);2001年04期

10 宋銘忻,路義鑫,周源昌,李淑聲,宋誠(chéng),林明亮,羅公平,姜淑珍,胡艷君,張彥慶,胡天陽(yáng),盧學(xué)利,石金峰;旋毛蟲病幾種檢測(cè)方法的比較研究[J];肉品衛(wèi)生;1998年08期

本文編號(hào)：2469461