【摘要】：目的觀察鈣反應(yīng)性反式激活因子(CREST)過(guò)表達(dá)對(duì)小鼠神經(jīng)母細(xì)胞瘤(N2a)細(xì)胞突起生長(zhǎng)的影響。方法實(shí)驗(yàn)分為3組,實(shí)驗(yàn)組用pCMV-HA-CREST轉(zhuǎn)染的N2a細(xì)胞,空載體對(duì)照組用pCMV-HA轉(zhuǎn)染的N2a細(xì)胞,空白對(duì)照組用未轉(zhuǎn)染任何試劑的N2a細(xì)胞,分別加入70mmol/L KCl孵育過(guò)夜。在轉(zhuǎn)染后24h,48h采用考馬斯亮藍(lán)染色法檢測(cè)N2a細(xì)胞突起生長(zhǎng)狀態(tài)。結(jié)果在轉(zhuǎn)染后24h,CREST基因過(guò)表達(dá)的細(xì)胞與兩對(duì)照組細(xì)胞相比較,細(xì)胞突起生長(zhǎng)差異無(wú)顯著性。在轉(zhuǎn)染后48h,CREST基因過(guò)表達(dá)的細(xì)胞與兩對(duì)照組細(xì)胞相比較,突起生長(zhǎng)明顯,部分突起交織成網(wǎng)絡(luò)。兩對(duì)照組間在細(xì)胞轉(zhuǎn)染后各時(shí)間點(diǎn)突起生長(zhǎng)狀態(tài)差異無(wú)顯著性(P0.05)。結(jié)論過(guò)表達(dá)CREST可促進(jìn)N2a細(xì)胞突起的生長(zhǎng)。
[Abstract]:Aim to investigate the effect of over-expression of calcium-responsive trans-activator (CREST) on neurite growth of mouse neuroblastoma (N2a) cells. Methods the experiment was divided into three groups: N2a cells transfected with pCMV-HA-CREST in experimental group, N2a cells transfected with pCMV-HA in empty vector control group, N2a cells transfected with pCMV-HA in blank control group and N2a cells transfected with no reagent in blank control group. 70mmol/L KCl was added to incubate N2a cells for the night. The neurite growth of N2a cells was detected by Coomassie brilliant blue staining at 24 h and 48 h after transfection. Results at 24 h after transfection, there was no significant difference in the neurite growth between the cells overexpressed by crest gene and those of the two control cells. At 48 h after transfection, the crest gene over-expressed cells grew significantly compared with the two control cells, and some of the processes were interwoven into a network. There was no significant difference in the neurite growth status between the two control groups at each time point after transfection (P0.05). Conclusion overexpression of CREST can promote the neurite growth of N2a cells.
【作者單位】： 山東萬(wàn)杰醫(yī)學(xué)院組織學(xué)與胚胎學(xué)教研室;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院組織學(xué)與胚胎學(xué)教研室;
【分類號(hào)】：R329.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 郭國(guó)慶;辛莉;邱小忠;沈偉哉;原林;;CRMP-1真核表達(dá)載體的構(gòu)建及其突起生長(zhǎng)抑制作用[J];生物化學(xué)與生物物理進(jìn)展;2009年03期

2 余曉君,王s，

本文編號(hào)：2461685