【摘要】：目的 以高產(chǎn)β-半乳糖苷酶(β-galactosidase)的保加利亞乳桿菌(Lactobacillus bulgaricus)β-半乳糖苷酶基因(lacZ)為初始基因，遞歸PCR合成的乳酸乳球菌基因usp45為分泌信號(hào)肽基因，表達(dá)型質(zhì)粒pMG36e為載體，構(gòu)建重組質(zhì)粒pMG36e-lacZ和pMG36e-usp-lacZ。電轉(zhuǎn)化乳酸乳球菌MG1363，獲得能分泌高活性β-半乳糖苷酶的重組乳酸乳球菌，并初步研究其產(chǎn)酶特性。為進(jìn)一步構(gòu)建食品級(jí)重組乳酸桿菌，用于乳糖酶缺乏的治療和研究奠定基礎(chǔ)。 方法 本研究分5個(gè)部分： 1．β-半乳糖苷酶基因初始菌的鑒定及其產(chǎn)酶特性研究：對(duì)基因初
[Abstract]:Objective to use the 尾-galactosidase (尾-galactosidase)-producing (Lactobacillus bulgaricus) 尾-galactosidase gene (lacZ) as the initial gene of Lactobacillus bulgaricus. The gene usp45 of Lactococcus lactis synthesized by recursive PCR was used as the secretory signal peptide gene, and the expression plasmid pMG36e was used as the vector. The recombinant plasmids pMG36e-lacZ and pMG36e-usp-lacZ. were constructed. The recombinant lactococcus, which can secrete high activity 尾-galactosidase, was obtained by electrotransformation of Lactococcus lactis MG1363,. It lays a foundation for the further construction of food-grade recombinant lactobacillus for the treatment and research of lactase deficiency. Methods this study was divided into five parts: 1. Identification of 尾-galactosidase gene and characterization of 尾-galactosidase gene: characterization of 尾-galactosidase gene
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R371

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 羅冬英,尹傳武;乳酸菌制劑對(duì)人體保健功效的機(jī)理探討[J];鄂州大學(xué)學(xué)報(bào);2002年04期

2 鐘燕;乳糖不耐受基礎(chǔ)研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(衛(wèi)生學(xué)分冊(cè));2000年03期

3 焦振泉,劉秀梅;16s rRNA序列同源性分析與細(xì)菌系統(tǒng)分類鑒定[J];國(guó)外醫(yī)學(xué)(衛(wèi)生學(xué)分冊(cè));1998年01期

4 王麗影，葉勤，，張宏，張嗣良, 吳自榮，王林發(fā);基因工程枯草桿菌生產(chǎn)中性蛋白酶的研究[J];華東理工大學(xué)學(xué)報(bào);1995年06期

5 李文,張雪梅,盧陽(yáng),曾s

本文編號(hào)：2443345