【摘要】：目的 以高產(chǎn)β-半乳糖苷酶(β-galactosidase)的保加利亞乳桿菌(Lactobacillus bulgaricus)β-半乳糖苷酶基因(lacZ)為初始基因，遞歸PCR合成的乳酸乳球菌基因usp45為分泌信號肽基因，表達型質(zhì)粒pMG36e為載體，構建重組質(zhì)粒pMG36e-lacZ和pMG36e-usp-lacZ。電轉化乳酸乳球菌MG1363，獲得能分泌高活性β-半乳糖苷酶的重組乳酸乳球菌，并初步研究其產(chǎn)酶特性。為進一步構建食品級重組乳酸桿菌，用于乳糖酶缺乏的治療和研究奠定基礎。 方法 本研究分5個部分： 1．β-半乳糖苷酶基因初始菌的鑒定及其產(chǎn)酶特性研究：對基因初
[Abstract]:Objective to use the 尾-galactosidase (尾-galactosidase)-producing (Lactobacillus bulgaricus) 尾-galactosidase gene (lacZ) as the initial gene of Lactobacillus bulgaricus. The gene usp45 of Lactococcus lactis synthesized by recursive PCR was used as the secretory signal peptide gene, and the expression plasmid pMG36e was used as the vector. The recombinant plasmids pMG36e-lacZ and pMG36e-usp-lacZ. were constructed. The recombinant lactococcus, which can secrete high activity 尾-galactosidase, was obtained by electrotransformation of Lactococcus lactis MG1363,. It lays a foundation for the further construction of food-grade recombinant lactobacillus for the treatment and research of lactase deficiency. Methods this study was divided into five parts: 1. Identification of 尾-galactosidase gene and characterization of 尾-galactosidase gene: characterization of 尾-galactosidase gene
【學位授予單位】：四川大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R371

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