【摘要】：前言 紫菜屬于紅藻類(Rhodophyta),紅毛菜目(Bangiales),紅毛菜科(Bangiaceae),紫菜屬(Porphyra)。紫菜多糖(PP)是紫菜的重要組成成分,研究表明具有多種生物學(xué)功能,如抗凝血、降血脂、抗血栓、,抗炎、防治潰瘍等作用,以及明顯的抗突變、抗腫瘤作用。近年來(lái)紫菜多糖作為一種免疫調(diào)節(jié)劑逐漸被人們所認(rèn)識(shí),其可上調(diào)機(jī)體的免疫系統(tǒng)功能,刺激各種免疫活性細(xì)胞如自然殺傷細(xì)胞和B細(xì)胞成熟、分化和增殖,并能促進(jìn)外周血淋巴細(xì)胞的轉(zhuǎn)化,可以促進(jìn)脾細(xì)胞分泌細(xì)胞毒效應(yīng)分子TNF,使其細(xì)胞毒作用得到增強(qiáng)。 紫菜多糖主要由巖藻糖,半乳糖,甘露糖,葡萄糖,和木糖組成,是一種含糖醛酸的異多糖,典型的紫菜聚糖結(jié)構(gòu)含有3,6-內(nèi)醚-半乳糖基和硫酸基,研究表明,后者與紫菜多糖所具有的免疫調(diào)節(jié)作用關(guān)系密切。本實(shí)驗(yàn)通過(guò)給予小鼠腹腔注射紫菜多糖,取脾細(xì)胞檢測(cè)NK細(xì)胞殺傷活性,淋巴細(xì)胞增殖功能,及產(chǎn)生TNF的含量,以探討紫菜多糖對(duì)免疫功能的影響。 實(shí)驗(yàn)材料與方法 將干燥紫菜以蒸餾水浸濕,95%酒精脫脂,蒸餾水浸泡以除色素,以10倍水量文火煮沸提取,以三氯乙酸法(TCA法)除蛋白,無(wú)水乙醇沉淀多糖,冷凍干燥后得PP提取物。以苯酚-硫酸法檢測(cè)純度。將BALB/C小鼠隨機(jī)分為4組,每組18只:生理鹽水對(duì)照組,不同劑量PP處理組(0.05g·kg~(-1),0.25g·kg~(-1),0.5g·kg~(-1))連續(xù)免疫7天,分別于末次免疫后的24h,48h,72h,殺鼠各6只,無(wú)菌取脾,調(diào)細(xì)胞濃度至1×10~7/ml,用乳酸脫氫酶釋放法檢測(cè)NK細(xì)胞的活性,用MTT法檢測(cè)淋巴細(xì)胞轉(zhuǎn)化功能及脾培養(yǎng)上清中的TNF對(duì)靶細(xì)胞的殺傷活性,用SPSS統(tǒng)計(jì)軟件對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行差異顯
[Abstract]:Introduction Laver belongs to red algae (Rhodophyta), (Bangiales), (Bangiaceae), (Porphyra). Porphyra polysaccharide (PP) is an important component of porphyra. Studies have shown that it has a variety of biological functions, such as anticoagulant, antithrombotic, anti-inflammatory, anti-ulcer, as well as obvious anti-mutation, anti-tumor effects. In recent years, porphyra polysaccharide has been gradually recognized as an immunomodulator, which can up-regulate the function of the body's immune system and stimulate the maturation, differentiation and proliferation of various immune active cells such as natural killer cells and B cells. It can promote the transformation of peripheral blood lymphocytes and enhance the cytotoxicity of spleen cells by secreting cytotoxic response molecule TNF,. Porphyra polysaccharide, mainly composed of fucose, galactose, mannose, glucose, and xylose, is an isopolysaccharide containing uronic acid. The typical porphyra polysaccharide structure contains 3,6-internal ether-galactosyl and sulfate groups. The latter is closely related to the immunomodulatory effect of porphyra polysaccharide. In order to explore the effect of porphyra polysaccharide on immune function, the cytotoxicity of NK cells, the proliferation of lymphocytes and the production of TNF were detected by injecting porphyra polysaccharide into mice intraperitoneally. Materials and methods the dried porphyra was soaked in distilled water, degreased by 95% alcohol, soaked in distilled water to remove pigment, boiled in 10 times water and removed protein by trichloroacetic acid (TCA) method. The polysaccharide was precipitated by anhydrous ethanol and the PP extract was obtained after freeze-drying. The purity was determined by phenol-sulfuric acid method. BALB/C mice were randomly divided into 4 groups: saline control group (n = 18), PP treated group (0.05g kg~ (- 1), 0.25g kg~ (- 1), 0.5g kg~ (- 1) for 7 days. At 24 h, 48 h, 72 h after the last immunization, 6 mice in each group were killed. The spleen was taken aseptically and the cell concentration was adjusted to 1 脳 10 ~ 7 ml. The activity of NK cells was measured by lactate dehydrogenase release assay. The transformation function of lymphocytes and the killing activity of TNF in the supernatant of spleen culture against target cells were detected by MTT method. The difference of the experimental results was observed by SPSS statistical software.
【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【引證文獻(xiàn)】

相關(guān)期刊論文 前2條

1 趙保力;張英鋒;李長(zhǎng)江;馬子川;;紫菜多糖的提取及化學(xué)成分[J];化學(xué)教學(xué);2010年03期

2 羅彤;戚向陽(yáng);李培培;;紫菜多糖雙向免疫調(diào)節(jié)作用的研究進(jìn)展[J];食品科技;2010年08期

相關(guān)碩士學(xué)位論文 前1條

1 黃麗嬌;荔枝多糖LCP50和LCP50W對(duì)小鼠的免疫功能調(diào)節(jié)作用研究[D];暨南大學(xué);2013年

，

本文編號(hào)：2440101