【摘要】：背景與目的 骨髓來源的間充質(zhì)干細(xì)胞(Mesenchymal stem cells,MSCs)是存在于骨髓中除造血干細(xì)胞(hemopoietic stem cells,HSCs)外的另一種干細(xì)胞,是中胚層發(fā)育的早期細(xì)胞。不僅參與生成造血微環(huán)境支持造血,在適宜的條件下還可分化為中胚層和神經(jīng)外胚層來源的組織細(xì)胞:成骨細(xì)胞、脂肪細(xì)胞、神經(jīng)細(xì)胞、肝細(xì)胞、內(nèi)皮細(xì)胞等。因其具有高度自我更新能力和多向分化潛能等優(yōu)點(diǎn),是細(xì)胞替代治療、基因治療理想的細(xì)胞來源。 現(xiàn)在MSCs的研究熱點(diǎn)是尋找高度特異性的分離純化方法、移植治療各系統(tǒng)功能障礙并重建組織功能、端粒酶活性的研究、基因治療的靶細(xì)胞,以及與MAPCs的比較研究等;對(duì)于MSCs誘導(dǎo)分化為神經(jīng)細(xì)胞的研究,也多停留于神經(jīng)細(xì)胞特異性標(biāo)記的檢測(cè)和MSCs動(dòng)物模型移植后行為學(xué)評(píng)定等。 Tau蛋白,是一種含量最高的神經(jīng)元微管相關(guān)蛋白(microtubule associated protein,MAPs)。正常情況下,Tau廣泛存在于神經(jīng)元的軸突中。Tau蛋白的主要功能在于促進(jìn)微管的形成和保持微管的穩(wěn)定性,降低微管蛋白的解離,并誘導(dǎo)微管成束。Tau蛋白還在神經(jīng)元的分化、成熟、內(nèi)分泌活性、受體活化過程中都起著重要的作用。Tau蛋白可以通過它的磷酸化狀態(tài)從時(shí)間和空間上調(diào)節(jié)微管的組裝;Tau蛋白的異常磷酸化可使神經(jīng)細(xì)胞發(fā)生功能紊亂而喪失正常生理功能,導(dǎo)致神經(jīng)系統(tǒng)疾病的發(fā)生(如阿爾茨海默病等)。 MSCs的準(zhǔn)確誘導(dǎo)分化是其應(yīng)用于臨床治療的基礎(chǔ),這需要對(duì)MSCs發(fā)育有關(guān)的信號(hào)調(diào)節(jié)、微環(huán)境影響作用進(jìn)行深入地研究。目前研究發(fā)現(xiàn),在體外MSCs
[Abstract]:Background & objective Bone marrow derived mesenchymal stem cells (Mesenchymal stem cells,MSCs) are another kind of stem cells which exist in bone marrow except hematopoietic stem cells (hemopoietic stem cells,HSCs). They are early cells of mesoderm development. It is not only involved in the production of hematopoietic microenvironment to support hematopoiesis, but also can differentiate into mesoderm and neuroectodermal tissue cells: osteoblasts, adipocytes, nerve cells, hepatocytes, endothelial cells and so on. Because of its high self-renewal ability and multi-directional differentiation potential, it is an ideal cell source for cell replacement therapy and gene therapy. At present, the research focus of MSCs is to find highly specific methods of isolation and purification, transplantation to treat dysfunction of various systems and reconstruction of tissue function, research on telomerase activity, target cells for gene therapy, and comparative study with MAPCs, and so on. For the study of MSCs-induced differentiation into nerve cells, the detection of neuron-specific markers and the assessment of behavior after transplantation of MSCs animal model are more important. Tau protein, is the highest content of neuronal microtubule-associated protein (microtubule associated protein,MAPs). The main function of Tau protein is to promote the formation of microtubule and maintain the stability of microtubule, reduce the dissociation of microtubule protein, and induce microtubule bundle formation. Tau protein is still in the differentiation of neurons, and the main function of tau protein is to promote the formation of microtubule and maintain the stability of microtubule. Tau protein plays an important role in the process of maturation endocrine activity and receptor activation. Tau protein can regulate the assembly of microtubule in time and space through its phosphorylation state. Abnormal phosphorylation of Tau protein can cause dysfunction of neural cells and loss of normal physiological function, leading to the occurrence of nervous system diseases (such as Alzheimer's disease). The accurate induction and differentiation of MSCs is the basis of its application in clinical treatment, which requires in-depth study on the signal regulation and microenvironmental effects of MSCs development. At present, it has been found that in vitro MSCs
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 胡以平;干細(xì)胞:生物醫(yī)藥研究的新靶標(biāo)[J];第二軍醫(yī)大學(xué)學(xué)報(bào);2002年11期

2 王磊,袁錦楣,郝洪軍,李海峰,金弘敏,冀成君;阿爾茨海默病患者腦脊液中Tau蛋白的含量及意義[J];中國(guó)臨床神經(jīng)科學(xué);2000年04期

3 王亭忠,馬愛群;干細(xì)胞分化及其調(diào)控機(jī)制研究進(jìn)展[J];心臟雜志;2004年05期

4 陳運(yùn)賢,歐瑞明,鐘雪云,鐘立業(yè),陳惠珍,彭龍?jiān)?曾武濤,靳三慶,張希,韓忠朝;粒細(xì)胞集落刺激因子動(dòng)員骨髓干細(xì)胞治療大鼠急性心肌梗塞[J];中國(guó)病理生理雜志;2002年01期

5 張麗蓉,項(xiàng)鵬,夏文杰,陳振光,張秀明,李艷,李樹濃;PD98059對(duì)人骨髓間質(zhì)干細(xì)胞分化為成骨細(xì)胞的影響[J];中國(guó)病理生理雜志;2002年08期

6 肖慶忠,李浩威,溫冠媚,黃少華,張秀明,李艷,李樹濃;麝香多肽體外誘導(dǎo)成年大鼠和人骨髓間充質(zhì)干細(xì)胞定向分化為神經(jīng)元的研究[J];中國(guó)病理生理雜志;2002年10期

7 宮立眾,孫士紅,程天民,粟永萍,羅成基,郭朝華;骨髓間質(zhì)干細(xì)胞輸注對(duì)外周血干細(xì)胞移植造血恢復(fù)的影響[J];中國(guó)病理生理雜志;2002年11期

8 陳運(yùn)賢,鐘雪云,歐瑞明;造血干細(xì)胞的可塑性及臨床應(yīng)用[J];中國(guó)病理生理雜志;2002年12期

，

本文編號(hào)：2436926