【摘要】：前言 治療性血管新生(therapeutic angiogenesis)是通過血管生成促進因子的直接應用,特異性轉(zhuǎn)基因治療及細胞治療以促進缺血組織血管生成,改善心肌血供為目的的治療。缺血性心臟病的治療目的是擴張冠狀動脈,促進血管新生,改善心肌血供。多種血管生成促進因子參與心臟新生血管形成,用于治療性血管新生。其中,血管內(nèi)皮生長因子(vascular endothelial growth factor,VEGF)和堿性成纖維細胞生長因子(basic fibroblast growth factor,bFGF)是最重要的并且研究較多的兩種血管生成促進因子。本實驗在體外培養(yǎng)大鼠心臟微血管內(nèi)皮細胞(cardiac microvascular endothelial cell,CMEC),并應用Matrigel建立誘導血管腔樣結(jié)構(gòu)形成的體外培養(yǎng)體系,研究血管生成促進因子bFGF對誘導心臟微血管內(nèi)皮細胞形成管腔樣結(jié)構(gòu)的影響,和bFGF對內(nèi)皮細胞VEGF受體Flt-1的作用,進而探討bFGF促進血管形成過程的作用機制。 方法 首先,參考Nishida等的實驗方法,分離、培養(yǎng)大鼠心臟微血管內(nèi)皮細胞。根據(jù)不同的實驗因素濃度設計成濃度效應組:不同濃度bFGF(5,10,20,40ng/ml)和正常對照組(無血清DMEM培養(yǎng)液),分別作用24小時。應用Matrigel誘導管腔樣結(jié)構(gòu)形成的體外培養(yǎng)體系,檢測不同濃度bFGF作用后,心臟微血管內(nèi)皮細胞形成管腔的情況。RT-PCR檢測各組血管內(nèi)皮細胞的Flt-1mRNA表達量。最后統(tǒng)計分析結(jié)果。 結(jié)果 實驗結(jié)果表明,應用Matrigel可誘導心臟微血管內(nèi)皮細胞管腔樣結(jié)構(gòu)
[Abstract]:The purpose of therapeutic angiogenesis (therapeutic angiogenesis) is to promote angiogenesis in ischemic tissues through the direct application of angiogenic promoters, specific transgene therapy and cell therapy. To improve myocardial blood supply for the purpose of treatment. The aim of treatment of ischemic heart disease is to dilate coronary artery, promote angiogenesis and improve myocardial blood supply. A variety of angiogenesis promoters are involved in cardiac neovascularization and are used for therapeutic angiogenesis. Among them, vascular endothelial growth factor (vascular endothelial growth factor,VEGF) and basic fibroblast growth factor (basic fibroblast growth factor,bFGF) are the two most important and studied angiogenesis promoting factors. In this study, rat cardiac microvascular endothelial cells (cardiac microvascular endothelial cell,CMEC) were cultured in vitro, and Matrigel was used to establish an in vitro culture system to induce the formation of vascular lumen-like structure. To study the effect of angiogenesis promoting factor bFGF on the formation of lumen-like structure in cardiac microvascular endothelial cells and the effect of bFGF on VEGF receptor Flt-1 in endothelial cells, and to explore the mechanism of bFGF promoting angiogenesis. Methods firstly, the microvascular endothelial cells of rat heart were isolated and cultured according to the experimental methods of Nishida et al. Different concentration of bFGF (5,10,20,40 ng / ml) and normal control group (serum-free DMEM medium) were designed for 24 hours respectively according to the concentration of the experimental factors and the concentration of the control group (5 ng / ml, 10 ng / ml, 20 ng / ml, 40 ng / ml). In vitro culture system induced by Matrigel to induce lumen-like structure was used to detect the formation of lumen in cardiac microvascular endothelial cells treated with different concentrations of bFGF, and Flt-1mRNA expression in vascular endothelial cells in each group was measured by RT-PCR. Finally, statistical analysis of the results. Results the results showed that Matrigel could induce the lumen-like structure of cardiac microvascular endothelial cells.
【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R363.2

【參考文獻】

相關期刊論文 前2條

1 王冬,葉星沈;缺血性疾病與治療性血管再生[J];基礎醫(yī)學與臨床;2004年03期

2 金惠銘;治療性血管新生研究的若干進展[J];國外醫(yī)學(生理、病理科學與臨床分冊);2003年04期

，

本文編號：2430265