【摘要】：目的 研究原癌基因c-fos在正常小鼠大腦顳葉皮質、海馬和體外培養(yǎng)小鼠NIH3T3成纖維細胞中的生物節(jié)律變化,并闡述其與嗎啡成癮的關系。 方法 飼養(yǎng)正常小鼠,按時間點(間隔4小時)灌注固定小鼠,取全腦作冰凍切片,用原位雜交法測定小鼠顳葉皮質和海馬c-fos mRNA的變化。 體外培養(yǎng)小鼠NIH3T3成纖維細胞,血清休克誘導基因節(jié)律表達,按時間點提取細胞總RNA,用RT-PCR方法半定量檢測c-fos mRNA和per1 mRNA的節(jié)律變化。 制備嗎啡成癮小鼠模型,一組按時間點(間隔4小時)灌注固定小鼠,取全腦作冰凍切片,用原位雜交法測定小鼠顳葉皮質和海馬c-fos mRNA的變化;另一組按時間點注射鹽酸納絡酮催癮,連續(xù)觀察30min戒斷體征指標(跳躍、濕狗樣抖動、爪顫、齒顫)出現(xiàn)次數(shù)。
[Abstract]:Objective to study the biological rhythm of proto-oncogene c-fos in the temporal cortex, hippocampus and cultured NIH3T3 fibroblasts of normal mice, and to elucidate the relationship between proto-oncogene c-fos and morphine addiction. Methods normal mice were fed and fixed by perfusion at 4 hours interval. The whole brain was taken as frozen sections and the changes of c-fos mRNA in temporal cortex and hippocampus of mice were measured by in situ hybridization. Mouse NIH3T3 fibroblasts were cultured in vitro. The expression of shock induced gene was induced by serum. The total RNA, was extracted from the cells at time point and the changes of c-fos mRNA and per1 mRNA were detected by RT-PCR method. The morphine-dependent mice model was established. The mice were infused and fixed at 4 hours interval. The whole brain was taken as frozen sections and the changes of c-fos mRNA in temporal cortex and hippocampus were measured by in situ hybridization. The other group was injected with naloxone hydrochloride to induce addiction according to time point. The occurrence times of 30min abstinence signs (jumping, wet dog shaking, claw fibrillation, tooth tremor) were observed continuously.
【學位授予單位】：四川大學
【學位級別】：博士
【學位授予年份】：2005
【分類號】：R33

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