【摘要】：【背景和目的】 皮膚廣泛分布于脊椎動物和人體表面,其表皮的基底層內(nèi)分布著大量的淺感受器和豐富的神經(jīng)末梢。發(fā)育神經(jīng)生物學研究表明,皮膚是脊神經(jīng)感覺纖維的靶區(qū),在胚胎發(fā)育期間背根神經(jīng)節(jié)細胞的周圍突伸向皮膚特定的層次內(nèi),形成密集的神經(jīng)網(wǎng)絡(luò),說明皮膚的發(fā)育與周圍神經(jīng)感覺纖維的發(fā)育之間一定存在著某種密切的關(guān)系。然而有關(guān)胚胎發(fā)育時期皮膚細胞與發(fā)育中的神經(jīng)末梢之間聯(lián)系的建立,背根神經(jīng)節(jié)細胞的周圍突如何被誘導向靶區(qū)生長及調(diào)節(jié)因素等問題都不是很清楚。 一氧化氮(Nitric Oxide,NO)是一種在細胞內(nèi)和細胞間具有高度活性的信使分子,廣泛分布于生物體內(nèi)各組織中,特別是神經(jīng)組織中。關(guān)于NO在中樞和外周神經(jīng)系統(tǒng)的分布特點已有許多研究報道,但對胚胎期小鼠周圍神經(jīng)末梢的分布和發(fā)育未見報道。 因此,我們利用逆行軸漿運輸?shù)脑韺晒饨?fluorogold,FG)和辣根過氧化物酶(horse radish peroxidase;horseradish peroxidase,HRP)在體注射到胚胎的羊水中,觀察胚胎體神經(jīng)和背根神經(jīng)節(jié)細胞是否含有熒光金或HRP,并通過
[Abstract]:[background and objective] skin is widely distributed on vertebrate and human surface, and a large number of superficial receptors and abundant nerve endings are distributed in the basal layer of the epidermis. Developmental neurobiology studies have shown that the skin is the target of the sensory fibers of the spinal nerve. During embryonic development, the processes around the dorsal root ganglion cells extend to a specific level of the skin, forming a dense neural network. It suggests that there must be a close relationship between the development of skin and the development of peripheral nerve sensory fibers. However, it is not clear that the relationship between the skin cells and the developing nerve endings during embryonic development, how the peripheral processes of dorsal root ganglion cells are induced to the target and the regulatory factors. Nitric oxide (Nitric Oxide,NO) is a highly active messenger molecule in cells and between cells, which is widely distributed in various tissues of organisms, especially in nerve tissues. The distribution of NO in central and peripheral nervous system has been reported, but the distribution and development of peripheral nerve endings in embryonic mice have not been reported. Therefore, we use the principle of retrograde axonal transport to transfer fluorogold,FG and horseradish peroxidase (horse radish peroxidase;. Horseradish peroxidase,HRP) injected into the amniotic fluid of the embryo in vivo to observe whether the embryonic somatic nerve and dorsal root ganglion cells contain fluorescent gold or HRP, and pass through
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R329

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本文編號：2385137