【摘要】：目的： 神經(jīng)細(xì)胞移植是中樞神經(jīng)系統(tǒng)(central nervous system，CNS)退行性疾病、遺傳性疾病和中樞神經(jīng)系統(tǒng)損傷引起的神經(jīng)系統(tǒng)功能障礙最佳的治療方案。干細(xì)胞作為神經(jīng)細(xì)胞移植的來(lái)源，日益受到人們的關(guān)注。目前，關(guān)于體外誘導(dǎo)骨髓間充質(zhì)干細(xì)胞(bone mesenchymal stem cells，BMSCs)分化為神經(jīng)細(xì)胞的研究報(bào)道已有很多，但是，大多數(shù)研究是集中于神經(jīng)細(xì)胞標(biāo)志物，如NSE、NF-M等的檢測(cè)，神經(jīng)受體方面的研究鮮有報(bào)道。神經(jīng)受體是神經(jīng)遞質(zhì)發(fā)揮功能所必需的結(jié)構(gòu)，在跨膜信號(hào)轉(zhuǎn)導(dǎo)過(guò)程中，各種胞膜受體是細(xì)胞接受內(nèi)外環(huán)境刺激的門戶。不同的化學(xué)信號(hào)甚至相同的化學(xué)信號(hào)會(huì)因?yàn)槟な荏w的不同而對(duì)組織細(xì)胞產(chǎn)生截然不同的生理作用。因此，BMSCs誘導(dǎo)分化形成的神經(jīng)細(xì)胞是否表達(dá)神經(jīng)受體對(duì)于神經(jīng)細(xì)胞能否發(fā)揮正常功能是至關(guān)重要的。 多巴胺D2受體(dopamine receptor D2，DRD2)既是突觸前受體又是突觸后受體，，作為突觸前受體可以調(diào)節(jié)多巴胺的合成和釋放，作為突觸后受體可以調(diào)節(jié)DA能神經(jīng)元的電生理功能。5-羥色胺2(5-hydroxytryptamine 2，5-HT2)受體是突觸后受體，能抑制放電活動(dòng)，主要位于非5-HT能神經(jīng)元上，是許多抗精神病藥物的靶受體。γ-氨基丁酸(gamma-aminobutyric acid，GABA)受體是腦內(nèi)最普遍的抑制性神經(jīng)遞質(zhì)受體，其主要的生理作用是調(diào)節(jié)思維活動(dòng)，抑制憂慮和焦慮的產(chǎn)生。研究表明DRD2、GABA受體、5-HT受體在阿爾茨海默病(ALzheimer's desease，AD)患者腦內(nèi)表達(dá)下降。 本實(shí)驗(yàn)擬利用不同的細(xì)胞因子和化學(xué)試劑體外誘導(dǎo)人BMSCs向神經(jīng)細(xì)胞分化，然后檢測(cè)分化形成的神經(jīng)細(xì)胞表達(dá)GABA_A受體-α2和α3亞基、5-HT2受體
[Abstract]:Objective: nerve cell transplantation is the best treatment for neurodegenerative diseases, hereditary diseases and neurological dysfunction caused by central nervous system (central nervous system,CNS). Stem cells, as a source of neural cell transplantation, have attracted more and more attention. At present, there have been many reports about inducing the differentiation of bone marrow mesenchymal stem cells (bone mesenchymal stem cells,BMSCs) into nerve cells in vitro. However, most of the studies focus on the detection of neural cell markers, such as NSE,NF-M, etc. Few studies on nerve receptors have been reported. Neuroreceptors are the necessary structures for neurotransmitters to perform their functions. In the transmembrane signal transduction process, various cellular membrane receptors are the gateway for cells to be stimulated by internal and external environment. Different chemical signals and even the same chemical signals have different physiological effects on tissue cells due to different membrane receptors. Therefore, the expression of neuronal receptors in neurons induced by BMSCs is crucial to the normal function of neurons. Dopamine D2 receptor (dopamine receptor D2DRD2 is both a presynaptic receptor and a postsynaptic receptor. As a presynaptic receptor, dopamine synthesis and release can be regulated. As a postsynaptic receptor, it can regulate the electrophysiological function of DA neurons. 5-HT _ 2 receptor is a postsynaptic receptor that inhibits discharge activity and is mainly located in non-5-HT neurons. Gamma-aminobutyric acid (gamma-aminobutyric acid,GABA) receptor is the most common inhibitory neurotransmitter receptor in the brain. Its main physiological function is to regulate the activity of thinking and inhibit the production of anxiety and anxiety. Studies have shown that the expression of DRD2,GABA receptor, 5-HT receptor in the brain of patients with Alzheimer's disease (ALzheimer's desease,AD) decreased. In this study, different cytokines and chemical reagents were used to induce the differentiation of human BMSCs into neural cells in vitro, and then to detect the expression of GABA_A receptor- 偽 2 and 偽 3 subunits and 5-HT2 receptors in the differentiated neurons.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R329

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