發(fā)布時(shí)間：2018-10-13 20:13

【摘要】：為探討由B淋巴細(xì)胞刺激因子(BLyS)及其受體所介導(dǎo)的B細(xì)胞激活對(duì)疾病的致病機(jī)理，本研究根據(jù)Taqman技術(shù)的基本原理，在國(guó)內(nèi)率先建立了實(shí)時(shí)定量檢測(cè)BLyS受體mRNA含量的方法，并在國(guó)內(nèi)外首先對(duì)系統(tǒng)性紅斑狼瘡(SLE)和多發(fā)性骨髓瘤(MM)患者血清BLys蛋白和外周血單個(gè)核細(xì)胞(peripheral blood mononuclear cells，，PBMCs)BLys及其受體mRNA表達(dá)水平進(jìn)行檢測(cè)，初步探討B(tài)Lys及其受體在SLE和MM患者發(fā)病的作用；同時(shí)將檢測(cè)結(jié)果與兩個(gè)疾病其他臨床指標(biāo)進(jìn)行相關(guān)性研究，以探討B(tài)LyS及其受體在相關(guān)疾病的預(yù)后及治療等方面的意義；因?yàn)锽AFF-R為BLyS主要和特異性受體，我們構(gòu)建人BAFF-R 5′側(cè)翼區(qū)5′系列缺失的重組質(zhì)粒，為進(jìn)一步研究BAFF-R基因轉(zhuǎn)錄調(diào)控機(jī)制奠定基礎(chǔ)。
[Abstract]:In order to investigate the pathogenetic mechanism of B cell activation mediated by B lymphocyte stimulating factor (BLyS) and its receptor, a method of real-time and quantitative detection of BLyS receptor mRNA was established in China according to the basic principle of Taqman technique. The expression levels of serum BLys protein, peripheral blood mononuclear cell (peripheral blood mononuclear cells,PBMCs) BLys and its receptor mRNA in patients with systemic lupus erythematosus (SLE) and multiple myeloma (MM) were detected at home and abroad. To explore the role of BLys and its receptors in the pathogenesis of SLE and MM, and to study the correlation between the results of BLyS and other clinical indicators of the two diseases, and to explore the significance of BLyS and its receptors in the prognosis and treatment of related diseases. Because BAFF-R is the main and specific receptor for BLyS, we constructed the recombinant plasmid of 5 '-deletion of human BAFF-R 5'flanking region, which laid a foundation for further study on the transcriptional regulation mechanism of BAFF-R gene.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

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