【摘要】：目的探討ghrelin對大鼠消化間期復(fù)合肌電活動(IMC)的影響及其機(jī)制。方法采用免疫組織化學(xué)和圖像分析方法觀察ghrelin受體(生長激素促分泌素受體,GHS-R)在大鼠消化道的分布特征;采用多導(dǎo)生理記錄儀監(jiān)測大鼠消化間期IMC,觀察靜脈給予ghrelin對胃腸IMC的影響;采用放射免疫法測定靜脈給予ghrelin前后,IMC不同時(shí)相的血漿胃動素濃度。結(jié)果①GHS-R在胃腸道的腸肌叢和黏膜下叢有表達(dá),胃腺體均有GHS-R免疫強(qiáng)陽性廣泛表達(dá),在腸腺體的陽性表達(dá)較少。②靜脈給予ghrelin促進(jìn)胃腸IMC,IMC周期縮短,Ⅲ相時(shí)程縮短,Ⅲ相占IMC周期百分比無顯著性改變;靜脈注射ghrelin后,血漿胃動素周期性波動規(guī)律不受影響,各相應(yīng)時(shí)相胃動素濃度與ghrelin作用前比較無顯著性差異。結(jié)論 GHS-R主要分布于大鼠消化道的腸肌叢和黏膜下叢,在胃腺體有免疫強(qiáng)陽性表達(dá),在小腸腺有少量陽性表達(dá)。Ghrelin可促進(jìn)胃腸運(yùn)動,此作用與胃動素沒有明顯關(guān)系。
[Abstract]:Objective to investigate the effect and mechanism of ghrelin on interdigestive complex myoelectric activity (IMC) in rats. Methods Immunohistochemistry and image analysis were used to observe the distribution of ghrelin receptor (GHS-R) in the digestive tract of rats. The effects of intravenous administration of ghrelin on gastrointestinal IMC in rats were observed by multichannel physiological recorder during interdigestive period, and plasma motilin concentrations at different phases before and after ghrelin administration were measured by radioimmunoassay. Results 1GHS-R was expressed in myenteric plexus and submucosal plexus of gastrointestinal tract, and GHS-R immunoreactivity was widely expressed in gastric glands. The positive expression of ghrelin in intestinal gland was less than that in intestinal gland. The cycle of gastrointestinal IMC,IMC was shortened by intravenous administration of ghrelin, and the duration of phase 鈪，

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