發(fā)布時(shí)間：2018-09-05 13:29

【摘要】： 目的:抗生素所致腹瀉是抗生素應(yīng)用過程中發(fā)生的一種副作用。有實(shí)驗(yàn)證明抗生素應(yīng)用可以引起腸道菌群結(jié)構(gòu)紊亂,細(xì)菌易位現(xiàn)象發(fā)生及免疫功能受到抑制。本實(shí)驗(yàn)采用鹽酸林可霉素制造動(dòng)物腹瀉模型,采用大腸桿菌、活菌制劑干預(yù),其目的是①采用細(xì)菌培養(yǎng)、鑒定、G染色及厭氧菌代謝產(chǎn)物的氣相色譜分析等方法動(dòng)態(tài)觀察抗生素致腹瀉大鼠3-14天的腸道菌群結(jié)構(gòu)及遠(yuǎn)隔器官的細(xì)菌易位現(xiàn)象。從微生態(tài)角度出發(fā)解釋細(xì)菌易位現(xiàn)象的發(fā)生機(jī)制。②利用反轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)技術(shù)測(cè)定抗生素致腹瀉大鼠腸粘膜組織、腸系膜淋巴結(jié)(MLN)、肝臟、脾臟中Toll-like recepters (TLRs)mRNA的表達(dá)。說明抗生素應(yīng)用對(duì)模式識(shí)別受體機(jī)制(pattern recognition receptors mechanism,PRRs機(jī)制)的影響,分析抗生素應(yīng)用,細(xì)菌易位及免疫功能的關(guān)系。探討模式識(shí)別受體機(jī)制在機(jī)體防御機(jī)制:天然免疫和獲得性免疫中的重要作用。③采用活菌制劑乳酸桿菌灌胃的方法對(duì)比觀察腹瀉后菌群結(jié)構(gòu)、細(xì)菌易位及免疫功能的變化,以了解活菌制劑的干預(yù)效應(yīng)。④采用條件致病菌埃希氏致病性大腸桿菌灌胃的方法對(duì)比觀察細(xì)菌易位、腸道免疫功能的變化,以了解抗生素應(yīng)用造成的二重感染對(duì)細(xì)菌易位和免疫功能的影響。 方法:采用健康SD大鼠60只,雌雄各半,體重220-240
[Abstract]:Objective: antibiotic-induced diarrhea is a side effect of antibiotic application. Some experiments have proved that the use of antibiotics can cause intestinal microflora structure disorder, bacterial translocation phenomenon and the inhibition of immune function. In this experiment, lincomycin hydrochloride was used to make animal diarrhea model, Escherichia coli and live bacteria preparation were used to intervene. The purpose of this experiment was to use bacteria culture. The intestinal flora structure and bacterial translocation in distant organs of antibiotic induced diarrhea rats were dynamically observed by means of G staining and gas chromatographic analysis of anaerobes metabolites. The mechanism of bacterial translocation was explained from the microecological point of view. The expression of Toll-like recepters (TLRs) mRNA in intestinal mucosa, mesenteric lymph node, liver and spleen of rats with antibiotic induced diarrhea was determined by reverse transcriptase polymerase chain reaction (RT-PCR). The effect of antibiotic application on the (pattern recognition receptors mechanism,PRRs mechanism of pattern recognition receptor was explained, and the relationship among antibiotic application, bacterial translocation and immune function was analyzed. To explore the important role of pattern recognition receptor mechanism in the defense of organism: .3 the changes of bacterial community structure, bacterial translocation and immune function after diarrhea were observed by the method of intragastric perfusion of Lactobacillus, a living bacterium preparation, in innate and acquired immunity. In order to understand the intervention effect of living bacteria preparation, the method of oral administration of Escherichia coli was used to observe the changes of bacterial translocation and intestinal immune function. To understand the effect of double infection caused by antibiotic application on bacterial translocation and immune function. Methods: 60 healthy SD rats, half male and female, weight 220-240 were used.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李紅云,姚詠明,盛志勇;Toll樣受體與膿毒癥的研究進(jìn)展[J];中華燒傷雜志;2002年05期

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本文編號(hào)：2224413