【摘要】：Ca~(2+)是細(xì)胞內(nèi)一種重要的信號(hào)分子,它在肌肉收縮、突觸傳遞、激素分泌、基因轉(zhuǎn)錄、細(xì)胞的存活和死亡等眾多重要的生理過(guò)程中發(fā)揮關(guān)鍵的作用。鈣火花作為胞內(nèi)鈣釋放的基本單元在心肌、平滑肌、骨骼肌等肌肉細(xì)胞上研究比較多,而在神經(jīng)細(xì)胞上研究的比較少。頸上神經(jīng)節(jié)是用來(lái)研究交感系統(tǒng)和突觸傳遞的一個(gè)很好的標(biāo)本,對(duì)于鈣火花的研究還沒(méi)有涉及過(guò)。 在本文的研究中,我們利用共聚焦顯微鏡在頸上交感神經(jīng)節(jié)細(xì)胞上探測(cè)神經(jīng)元鈣火花的存在,并分析其特性,比較頸上神經(jīng)節(jié)細(xì)胞與其它類型細(xì)胞鈣火花的異同點(diǎn)。取出生3～7天的SD大鼠頸上神經(jīng)節(jié)細(xì)胞做原代培養(yǎng),實(shí)驗(yàn)前用Fluo-4-AM熒光探針事先裝載培養(yǎng)了3～10天的細(xì)胞,在Zeiss 510倒置共聚焦顯微鏡下,用低濃度的caffeine作為胞內(nèi)鈣庫(kù)的刺激物來(lái)誘發(fā)鈣信號(hào)。我們發(fā)現(xiàn),1.5mM的caffeine可以在細(xì)胞的胞體和神經(jīng)突上誘發(fā)產(chǎn)生鈣火花。對(duì)鈣火花的參數(shù)分析發(fā)現(xiàn),頸上神經(jīng)節(jié)細(xì)胞的鈣火花有它自身的特點(diǎn):與經(jīng)
[Abstract]:Ca2 is an important signal molecule in cells, which plays a key role in many important physiological processes, such as muscle contraction, synaptic transmission, hormone secretion, gene transcription, cell survival and cell death. As the basic unit of intracellular calcium release, calcium sparks are studied more in muscle cells, smooth muscle, skeletal muscle and other muscle cells, but less in nerve cells. The superior cervical ganglion is a good specimen for the study of sympathetic system and synaptic transmission, but has not been involved in the study of calcium sparks. In the present study, we use confocal microscope to detect the existence of calcium sparks in the superior cervical sympathetic ganglion cells, and analyze their characteristics, and compare the differences of calcium sparks between the superior cervical ganglion cells and other types of cells. The supracervical ganglion cells of SD rats were taken out for primary culture after 3 days old for 7 days. Before the experiment, the cells were cultured with Fluo-4-AM fluorescence probe for 10 days. The cells were cultured under Zeiss 510 inverted confocal microscope. Low concentration of caffeine was used as the stimulation of intracellular calcium reservoir to induce calcium signal. We found that 1.5 mm of caffeine could induce calcium sparks in the cell body and neuronal process. The analysis of the parameters of calcium sparks shows that the calcium sparks of the superior cervical ganglion cells have their own characteristics:
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R33

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 丁艷平;摘除頸上神經(jīng)節(jié)對(duì)成年大鼠海馬齒狀回神經(jīng)再生及學(xué)習(xí)記憶的影響[D];蘭州大學(xué);2008年

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本文編號(hào)：2192728