湖北土家族15個(gè)STR基因座遺傳多態(tài)性研究
發(fā)布時(shí)間：2018-08-18 21:11
【摘要】： 目的:了解湖北土家族15個(gè)STR基因座(TH01、CSF1PO、HUMTPOX、FGA、vWA、D2S1338、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D19S433、D21S11)的遺傳多態(tài)性,探討該民族與國(guó)內(nèi)18個(gè)民族群體的遺傳關(guān)系。 材料與方法:湖北土家族無(wú)關(guān)個(gè)體血樣110例,用Chelex-100方法提取DNA,應(yīng)用AmpFl STR~(?)Identifiler~(Tm)kit熒光標(biāo)記復(fù)合PCR擴(kuò)增技術(shù)對(duì)樣本DNA15個(gè)STR基因座進(jìn)行擴(kuò)增,在ABI Prism 3100型DNA序列分析儀對(duì)擴(kuò)增產(chǎn)物進(jìn)行檢測(cè),GeneScan Analysis3.7和Genotyper3.7分析軟件對(duì)檢測(cè)結(jié)果進(jìn)行掃描分析。用人工方法(Excel軟件)計(jì)算各個(gè)基因座的遺傳多態(tài)性參數(shù);另外選擇與本研究9個(gè)STR基因座完全相同的18個(gè)國(guó)內(nèi)民族群體進(jìn)行群體遺傳學(xué)研究,根據(jù)他們的等位基因頻率算出遺傳距離后,利用MEGA3.1軟件的Neigllbour-Joining方法構(gòu)建系統(tǒng)發(fā)生樹。 結(jié)果:①遺傳多態(tài)性參數(shù):湖北土家族15個(gè)STR基因座中共檢測(cè)出132種等位基因,等位基因頻率分布在0.0045-0.5182之間;374種基因型,頻率分布在0.0091～0.3182之間;雜合度(H_o)分布在0.6364～0.9091,平均雜合度為0.7873、多態(tài)信息含量(PIC)分布在0.5549～0.8552、個(gè)體識(shí)別力(DP)分布在0.7954～0.9595、累積個(gè)體識(shí)別力(TDP)大于0.99999999998,非父排除率(EP)分布在0.3658～0.8510,累積非父排除率(CEP)大于0.999999992。②群體遺傳學(xué)研究:湖北土家族與地理位置位于其周邊的漢族群體間的遺傳距離普遍小于其與其他少數(shù)民族群體間的遺傳距離,與維吾爾族的遺傳距離最大。湖北土家族與其他18個(gè)民族群體的系統(tǒng)發(fā)生樹結(jié)果顯示湖北土家族首先和廣西苗族、廣西瑤族相聚,其次同四川漢族、江西漢族、浙江漢族、湖南漢族、河南漢族相聚,再次與安徽漢族、甘肅漢族、甘肅東鄉(xiāng)族、陜西漢族、江蘇漢族相聚,然后和拉薩藏族及云南彝族、云南納西族相聚,最后與蒙古族、朝鮮族、維吾爾族相聚。 結(jié)論:湖北土家族的15個(gè)STR基因座除TPOX基因座外,其他14個(gè)基因座都屬于高度多態(tài)性遺傳標(biāo)記。湖北土家族群體可能是以土家族的遺傳標(biāo)記為主的融合有其他民族遺傳特征的群體。
[Abstract]:Objective: to investigate the genetic polymorphism of 15 STR loci in Tujia nationality of Hubei Province (TH01 / CSF1POP HUMTPOXA FGAvWAA D2S1338 / D3S1358 / D5S818D7S820 / D8S1179 / D16S539D18S51D19S433D21S11) and to explore the genetic relationship between this population and 18 ethnic groups in China. Materials and methods: one hundred and ten unrelated blood samples from Tujia nationality in Hubei Province were extracted by Chelex-100 method. The DNA15 STR loci were amplified by AmpFl STR ~ (?) Identifiler~ (Tm) kit fluorescence labeled PCR amplification technique. GeneScan Analysis3.7 and Genotyper3.7 software were used to detect the amplified products by ABI Prism 3100 DNA sequence analyzer. The genetic polymorphism parameters of each locus were calculated by artificial method (Excel software), and the population genetics of 18 national populations identical to 9 STR loci in this study were studied. The genetic distance was calculated according to their allelic frequency and the phylogenetic tree was constructed by Neigllbour-Joining method of MEGA3.1 software. Results 1 genetic polymorphism parameters: 132 alleles were detected in 15 STR loci of Tujia nationality in Hubei Province. The allele frequencies were between 0.0045-0.5182 and 0.3182, respectively. The distribution of heterozygosity (Ho) was 0.6364 / 0.9091, the average heterozygosity was 0.7873, the polymorphic information content (PIC) was 0.5549 / 0.8552, the individual recognition power (DP) was 0.7954 / 0.9595, the cumulative individual recognition power (TDP) was greater than 0.999999999998, the non-paternal exclusion rate (EP) was 0.36580.8510, and the cumulative non-paternal exclusion rate (EP) was 0.36580.8510. (CEP) > 0.9999992.2 population genetic study: the genetic distance between the Tujia nationality and the Han population located in its vicinity was generally smaller than the genetic distance between the Tujia nationality and the other minority populations in Hubei Province. The genetic distance between Uygur and Uygur is the largest. The phylogenetic tree results of the Tujia and other 18 ethnic groups in Hubei show that the Tujia in Hubei first meet with the Miao and Yao ethnic groups in Guangxi, then with the Han nationality in Sichuan, Jiangxi, Zhejiang, Hunan and Henan. They met with the Han nationality of Anhui, Gansu Dongxiang, Shaanxi Han and Jiangsu Han, then with Lhasa Tibetans, Yunnan Yi, Yunnan Naxi, and finally with Mongolian, Korean and Uygur. Conclusion: the 15 STR loci of Tujia nationality in Hubei are highly polymorphic except TPOX loci. The Tujia population in Hubei Province may be a population with genetic characteristics of other nationalities, which is based on genetic markers of Tujia nationality.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R394
本文編號(hào)：2190689
[Abstract]:Objective: to investigate the genetic polymorphism of 15 STR loci in Tujia nationality of Hubei Province (TH01 / CSF1POP HUMTPOXA FGAvWAA D2S1338 / D3S1358 / D5S818D7S820 / D8S1179 / D16S539D18S51D19S433D21S11) and to explore the genetic relationship between this population and 18 ethnic groups in China. Materials and methods: one hundred and ten unrelated blood samples from Tujia nationality in Hubei Province were extracted by Chelex-100 method. The DNA15 STR loci were amplified by AmpFl STR ~ (?) Identifiler~ (Tm) kit fluorescence labeled PCR amplification technique. GeneScan Analysis3.7 and Genotyper3.7 software were used to detect the amplified products by ABI Prism 3100 DNA sequence analyzer. The genetic polymorphism parameters of each locus were calculated by artificial method (Excel software), and the population genetics of 18 national populations identical to 9 STR loci in this study were studied. The genetic distance was calculated according to their allelic frequency and the phylogenetic tree was constructed by Neigllbour-Joining method of MEGA3.1 software. Results 1 genetic polymorphism parameters: 132 alleles were detected in 15 STR loci of Tujia nationality in Hubei Province. The allele frequencies were between 0.0045-0.5182 and 0.3182, respectively. The distribution of heterozygosity (Ho) was 0.6364 / 0.9091, the average heterozygosity was 0.7873, the polymorphic information content (PIC) was 0.5549 / 0.8552, the individual recognition power (DP) was 0.7954 / 0.9595, the cumulative individual recognition power (TDP) was greater than 0.999999999998, the non-paternal exclusion rate (EP) was 0.36580.8510, and the cumulative non-paternal exclusion rate (EP) was 0.36580.8510. (CEP) > 0.9999992.2 population genetic study: the genetic distance between the Tujia nationality and the Han population located in its vicinity was generally smaller than the genetic distance between the Tujia nationality and the other minority populations in Hubei Province. The genetic distance between Uygur and Uygur is the largest. The phylogenetic tree results of the Tujia and other 18 ethnic groups in Hubei show that the Tujia in Hubei first meet with the Miao and Yao ethnic groups in Guangxi, then with the Han nationality in Sichuan, Jiangxi, Zhejiang, Hunan and Henan. They met with the Han nationality of Anhui, Gansu Dongxiang, Shaanxi Han and Jiangsu Han, then with Lhasa Tibetans, Yunnan Yi, Yunnan Naxi, and finally with Mongolian, Korean and Uygur. Conclusion: the 15 STR loci of Tujia nationality in Hubei are highly polymorphic except TPOX loci. The Tujia population in Hubei Province may be a population with genetic characteristics of other nationalities, which is based on genetic markers of Tujia nationality.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R394
【引證文獻(xiàn)】
相關(guān)碩士學(xué)位論文 前1條
1 范瑾;雪山蒙古族人群15個(gè)STR基因座遺傳多態(tài)性[D];遼寧醫(yī)學(xué)院;2012年
，本文編號(hào)：2190689
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