【摘要】： 目的：模擬人的動(dòng)脈硬化閉塞癥，制作一種下肢缺血的實(shí)驗(yàn)動(dòng)物模型，建立持久的、穩(wěn)定的缺血模型。 方法：選取雄性新西蘭白兔10只，結(jié)扎并切除左下肢從腹股溝韌帶至膝關(guān)節(jié)的股動(dòng)脈及其分支，造成左下肢缺血，術(shù)后按時(shí)對(duì)家兔患肢缺血程度及肢體變化予以評(píng)估，直至術(shù)后6周。 結(jié)果：家兔缺血側(cè)肢體一周以后開始出現(xiàn)肌肉萎縮，壞死，肢體潰瘍，，指甲增長(zhǎng)、增厚等。術(shù)后家兔患肢血壓明顯下降，左下肢／右下肢血壓率顯著下降(P＜0.05)。放射性同位素灌注掃描示：患肢血流量明顯減少，左下肢／右下肢血流量明顯降低，左下肢同位素興趣圖分布明顯低于右下肢。DSA血管造影示：術(shù)后左下肢／右下肢的細(xì)微側(cè)枝血管記數(shù)率從1.00±0.52增加至3.00±0.04，血管數(shù)量明顯增加(P＜0.05)。術(shù)后6周患側(cè)與健側(cè)肌肉的病理學(xué)觀察示：患側(cè)肌纖維變小、變少、血管側(cè)支增多。 結(jié)論：家兔下肢慢性缺血模型成功制作，與臨床動(dòng)脈硬化閉塞癥病例癥狀相似，表現(xiàn)為持久的、穩(wěn)定的左下肢缺血。
[Abstract]:Aim: to establish an experimental animal model of lower extremity ischemia by simulating human arteriosclerosis obliterans. Methods: ten male New Zealand white rabbits were selected and the femoral artery and its branches from inguinal ligament to knee joint were ligated and resected to cause ischemia of the left lower extremity. Up to 6 weeks after operation. Results: muscle atrophy, necrosis, ulcers, nail growth and thickening began to occur in the ischemic limbs after one week. After operation, the blood pressure of the affected limbs decreased significantly, and the blood pressure rate of the left / right lower limbs decreased significantly (P < 0.05). Radioisotope perfusion scan showed that the blood flow of the affected limb was significantly decreased, and that of the left / right lower extremities was significantly decreased. The distribution of isotopic interest map of left lower extremity was significantly lower than that of right lower extremity. DSA showed that the count rate of fine lateral branches of left lower limb / right lower extremity increased from 1.00 鹵0.52 to 3.00 鹵0.04, and the number of blood vessels increased significantly (P < 0.05). The pathological observation of the affected and healthy muscles at 6 weeks after operation showed that the muscle fibers of the affected side became smaller and less, and the collateral branches of the vessels increased. Conclusion: the model of chronic ischemia of rabbit's lower extremities is made successfully, which is similar to the clinical symptoms of arteriosclerosis obliterans, showing persistent and stable left lower extremity ischemia.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R-332

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