【摘要】：目的:探討丙酸睪酮對去勢和不去勢SD大鼠前列腺增生的影響。方法:8周齡雄性SD大鼠25只,隨機分為正常對照組(n=5)、不去勢模型組(n=15)及去勢模型組(n=5);不去勢模型組按注射丙酸睪酮劑量又分為不去勢低劑量組、中劑量組和高劑量組,注射劑量分別為1.25,2.5,5 mg/(kg·d);去勢模型組無菌切除雙側(cè)睪丸1周后注射丙酸睪酮5 mg/(kg·d);去勢模型組和不去勢模型組均連續(xù)注射丙酸睪酮4周,于末次給藥后分離大鼠前列腺,檢測其濕重、體積和臟器系數(shù);組織切片HE染色,光鏡下觀察前列腺組織變化。結(jié)果:不去勢小劑量組和中劑量組的前列腺濕重、體積和臟器系數(shù)增加,與正常對照組相比差異有統(tǒng)計學(xué)意義(P0.05),不去勢高劑量組和去勢組的前列腺體積和臟器系數(shù)與正常對照組相比,差異均具有統(tǒng)計學(xué)意義(P0.01);去勢組和不去勢組大鼠前列腺體積和腺腔大小均較正常對照組增大,且不去勢組前列腺腺體與周圍組織界限更清楚,腺腔內(nèi)乳頭狀突起也比去勢組更加明顯。結(jié)論:使用不去勢、皮下注射5 mg/(kg·d)丙酸睪酮的方法可有效的建立前列腺增生大鼠模型。
[Abstract]:Aim: to investigate the effect of testosterone propionate on prostatic hyperplasia in ovariectomized and non-castrated SD rats. Methods Twenty-five male Sprague-Dawley rats aged 8 weeks were randomly divided into normal control group (n = 5), no castration model group (n = 15) and ovariectomized model group (n = 5), and no castration model group was divided into no castration low dose group, middle dose group and high dose group according to the dose of testosterone propionate injection. The rats in ovariectomized group were injected with testosterone propionate 5 mg/ (kg d); for 1 week, and testosterone propionate was injected continuously for 4 weeks in the ovariectomized model group and the non-castrated model group. The prostatic gland was separated after the last administration and its wet weight was measured. Volume and organ coefficient, HE staining of tissue sections, changes of prostate tissue under light microscope. Results: the wet weight, volume and organ coefficient of prostate were increased in the low dose group and middle dose group. Compared with the normal control group, the prostate volume and organ coefficient of the non-ovariectomized high dose group and ovariectomized group were significantly higher than that of the normal control group (P0.05). The difference was statistically significant (P0.01), the volume of prostate and the size of glandular cavity in ovariectomized group and non-castrated group were larger than those in normal control group, and the boundary between prostate gland and surrounding tissue in non-castrated group was more clear than that in non-castrated group. The intraglandular papillary process was also more pronounced than that in the castrated group. Conclusion: subcutaneous injection of testosterone propionate for 5 mg/ (kg d) can effectively establish a rat model of benign prostatic hyperplasia (BPH).
【作者單位】： 寧夏醫(yī)科大學(xué)檢驗學(xué)院;寧夏醫(yī)科大學(xué)實驗動物中心;
【基金】：國家自然科學(xué)基金(81201344) 寧夏醫(yī)科大學(xué)面上項目(XM2011008)
【分類號】：R697.32;R-332

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