【摘要】： 鉛(Pb)、鎘(Cd)均為重要的環(huán)境污染物,并通過食物鏈系統(tǒng)進入動物和人體產生毒性作用。本研究是在建立SD大鼠腎細胞培養(yǎng)的基礎上,通過培養(yǎng)液中加入不同濃度的Cd(10、20、40μmol/L)、Pb(10,20,40μmol/L)和Pb-Cd聯(lián)合(分別為5、10、20μmol/L),并加入Na_2SeO_30.5mg/L,在培養(yǎng)6h后,觀察細胞形態(tài)、測定細胞凋亡率和細胞周期及上清液脂質過氧化指標,探討Pb、Cd對腎細胞的聯(lián)合毒性及硒的保護作用。結果表明: (1)建立了0.05%胰蛋白酶消化SD乳鼠腎組織培養(yǎng)腎細胞的方法,該法細胞產量高,操作簡便,不易污染,花費較少。 (2)低濃度Pb或Cd(10μmol/L)對細胞無明顯損傷,20μmol/L以上可以使細胞皺縮、體積變小,表面有細胞碎片,甚至呈泡沫狀或樹枝狀。Pb、Cd聯(lián)合可加劇細胞損傷。 (3)低濃度的Pb、Cd(10μmol/L)使細胞凋亡率下降,而20μmol/L以上的Pb、Cd均可使細胞凋亡率增加,并改變細胞周期。Pb、Cd聯(lián)合具有明顯的協(xié)同性,分別濃度達40μmol/L時,細胞凋亡率降低,表明細胞已有死亡。 (4)Pb、Cd均可引起腎細胞培養(yǎng)液GSH-Px、SOD的活性降低,MDA含量升高,并存在劑量和時間效應。 (5)在培養(yǎng)液中添加Na_2SeO_3,可降低腎細胞凋亡率,使細胞周期趨于正常,并緩解GSH-Px、SOD活性下降和MDA含量升高的幅度,表明Se對Pb、Cd所致的腎細胞損傷具有一定的保護作用。
[Abstract]:Lead, (Pb), cadmium and (Cd) are important environmental pollutants and have toxic effects on animals and human beings through food chain system. Based on the establishment of renal cell culture in SD rats, different concentrations of CD (10 ~ 20 ~ 40 渭 mol/L) Pb (10 ~ 20 ~ 20 ~ 40 渭 mol/L) and Pb-Cd (5 ~ 10 ~ 10 ~ 20 渭 mol/L) were added to the culture medium to observe the morphology of the cells, and Na2SeOO _ (30) mg / L was added to the culture medium. The apoptosis rate, cell cycle and lipid peroxidation in supernatant were measured to study the combined toxicity of PBN and CD on renal cells and the protective effect of selenium. The results showed that: (1) A method for digestion of kidney cells by 0.05% trypsin was established. The method has the advantages of high cell yield, simple operation and easy contamination. (2) low concentration of Pb or CD (10 渭 mol/L) had no obvious damage to the cells. More than 20 渭 mol/L could make the cells shrink, become smaller, and have cell fragments on the surface. (3) low concentration of PBN CD (10 渭 mol/L) decreased the apoptosis rate of cells, but more than 20 渭 mol/L of PBN CD could increase the apoptosis rate of the cells, and even the combination of foam or dendritic cells could aggravate the cell injury. (3) low concentration of PBN CD (10 渭 mol/L) decreased the apoptosis rate of the cells, but more than 20 渭 mol/L, the apoptosis rate of the cells increased. When the cell cycle was changed, the cell apoptosis rate decreased when the concentration of 40 渭 mol/L was 40 渭 mol/L, respectively. The results indicated that the cells had died. (4) PbPxC could induce the decrease of the activity of GSH-PxN SOD in renal cell culture medium and the increase of MDA content. There were dose and time effects. (5) adding NaCo2SeO3 to the culture medium could reduce the apoptosis rate of renal cells and make the cell cycle normal. It also alleviated the decrease of SOD activity and the increase of MDA content, indicating that se has a certain protective effect on renal cell injury induced by PBH and CD.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R363

【引證文獻】

相關期刊論文 前1條

1 沈雪;王昊;關爽;盧靜;;細胞毒性在食品安全性中的應用研究[J];食品科學;2008年09期

，

本文編號：2170007