【摘要】： 目的:Geotricum sivicola是地霉屬新發(fā)現的一個種,目前國內外有關的報道極少。2002年我科從一位膿癬病人的病發(fā)及痂皮部位首次分離出此菌,由中國醫(yī)學科學院微生物研究所鑒定為Geotricum sivicola,原翻譯為昔維考拉地霉,現根據其拉丁文原意譯為林生地霉。李秀麗等[1]從形態(tài)學、營養(yǎng)生理學、分子生物學等各個角度對林生地霉進行初步研究,并且通過皮膚感染的動物實驗及毛發(fā)受侵試驗對其致病力進行了初步探討。 本研究通過不同途徑給予不同免疫狀態(tài)下的昆明小鼠注射林生地霉,檢測是否能夠引起系統性的林生地霉感染,并且觀察不同免疫狀態(tài)下動物的死亡情況,同時對自然死亡和分期處死的小鼠進行解剖,通過直接鏡檢、組織逆培養(yǎng)、組織病理學檢查、組織載菌量測定等方法比較各臟器的感染程度以及電鏡觀察該菌在組織中的形態(tài)。為進一步了解此菌的致病力和引起的病理形態(tài)學改變提供實驗室依據。 方法: 1林生地霉系統感染動物模型的制作將我科保存菌種復溫,轉種在沙堡氏培養(yǎng)基上37℃培養(yǎng)一周。用無菌生理鹽水制備成菌懸液,通過皮下、腹腔、靜脈三種途徑接種至不同免疫狀態(tài)的小鼠。 2死亡率的觀察連續(xù)觀察各組小鼠感染該菌后4周內的自然死亡情況,記錄死亡日期和死亡只數。
[Abstract]:Objective: Geotricum sivicola is a newly discovered species of Trichoderma, which is rarely reported at home and abroad. In 2002, the strain was first isolated from the hair and callus of a patient with psoriasis purpura. Geotricum sivicola was identified by the Institute of Microbiology, Chinese Academy of Medical Sciences. Li Xiuli et al. [1] the pathogenicity of Trichoderma woodland was studied from the aspects of morphology, nutrition physiology and molecular biology, and its pathogenicity was studied by animal experiment of skin infection and hair invasion test. In this study, Kunming mice in different immune states were injected with Trichoderma plantarum in different ways to detect whether they could cause systemic infection of Trichoderma plantarum, and to observe the death of animals in different immune states. At the same time, the mice died by stages were dissected. The infection degree of the organs and the morphology of the bacteria in the tissues were observed by direct microscope examination, tissue reverse culture, histopathological examination and tissue carrying capacity measurement. To provide laboratory basis for further understanding the pathogenicity and pathomorphological changes caused by this bacterium. Methods: 1 the animal model of Trichoderma woodland system infection was established. The preserved strains of the family were reheated and transferred to the culture medium at 37 鈩，

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