【摘要】：目的：分離大鼠骨髓間充質(zhì)干細(xì)胞和脂肪間充質(zhì)干細(xì)胞，比較兩種細(xì)胞的特征，為脂肪間充質(zhì)干細(xì)胞可替代骨髓基質(zhì)細(xì)胞做為種子細(xì)胞提供依據(jù)。 方法：利用細(xì)胞貼壁性對(duì)骨髓間充質(zhì)干細(xì)胞和脂肪間充質(zhì)干細(xì)胞進(jìn)行分離，比較兩種細(xì)胞的情況，包括(1)單位質(zhì)量組織獲得兩種細(xì)胞的量、(2)細(xì)胞形態(tài)、(3)細(xì)胞貼壁率、(4)細(xì)胞動(dòng)力學(xué)、(5)細(xì)胞表面標(biāo)記。并對(duì)數(shù)據(jù)進(jìn)行分析。 結(jié)果：(1)單位質(zhì)量組織獲得骨髓間充質(zhì)干細(xì)胞數(shù)量為(2.23±0.35)×10~5／g，脂肪間充質(zhì)干細(xì)胞為(2.66±0.75)×10~5/g，采用成組設(shè)計(jì)兩樣本比較的t檢驗(yàn)，t=-1.446，P=0.179＞0.05，尚不能認(rèn)為兩者有差異，可以認(rèn)為在單位質(zhì)量骨髓和脂肪組織中獲得的骨髓間充質(zhì)干細(xì)胞和脂肪間充質(zhì)干細(xì)胞數(shù)量相當(dāng)。(2)兩種細(xì)胞形態(tài)均為條索樣，呈成纖維細(xì)胞形態(tài)。(3)應(yīng)用直線(xiàn)相關(guān)分析考察兩組細(xì)胞的貼壁率之間的關(guān)系，相關(guān)系數(shù)r=0.999，決定系數(shù)r~2=0.997，(F=5862.949，，P＜0.001)，可以認(rèn)為兩貼壁率之間有直線(xiàn)相關(guān)關(guān)系，可以認(rèn)為在不同的時(shí)間點(diǎn)兩種細(xì)胞的貼壁率相似，并且有共同遞增趨勢(shì)。(4)脂肪間充質(zhì)干細(xì)胞的增殖能力與骨髓間充質(zhì)干細(xì)胞相當(dāng)，消化后的第2、5代細(xì)胞經(jīng)歷24～48小時(shí)的潛伏期后，進(jìn)入6～9天的對(duì)數(shù)生長(zhǎng)期，然后過(guò)渡到平臺(tái)期。(5)通過(guò)流式實(shí)驗(yàn)和免疫細(xì)胞化學(xué)顯示，骨髓間充質(zhì)干細(xì)胞和骨髓間充質(zhì)干細(xì)胞表面表達(dá)CD29、CD44，不表達(dá)CD34。 結(jié)論：(1)自大鼠脂肪中可提取出與骨髓間充質(zhì)干細(xì)胞類(lèi)似的脂肪間充質(zhì)干細(xì)胞。(2)脂肪間充質(zhì)干細(xì)胞的表面標(biāo)記與骨髓間充質(zhì)干
[Abstract]:Aim: to isolate rat bone marrow mesenchymal stem cells (BMSCs) and adipose mesenchymal stem cells (AMSCs) and compare the characteristics of the two cells so as to provide a basis for replacing bone marrow stromal cells (BMSCs) with adipose mesenchymal stem cells (AMSCs) as seed cells. Methods: bone marrow mesenchymal stem cells (BMSCs) and adipose mesenchymal stem cells (AMSCs) were isolated with adherent cells. It includes (1) the quantity of two kinds of cells obtained by unit mass tissue, (2) cell morphology, (3) cell adhesion rate, (4) cell dynamics, and (5) cell surface labeling. The data are analyzed. Results: (1) the number of bone marrow mesenchymal stem cells (BMSCs) and adipose mesenchymal stem cells (BMSCs) were (2.23 鹵0.35) 脳 10 ~ (5) / g and (2.66 鹵0.75) 脳 10 ~ (5) / g respectively. It can be concluded that the number of bone marrow mesenchymal stem cells and adipose mesenchymal stem cells obtained in bone marrow and adipose tissue per unit mass is equal. (3) the linear correlation analysis was used to investigate the relationship between the adherent rate of the two groups, the correlation coefficient was 0.999, the determining coefficient was 0.997, and the coefficient of determination was 0.997 (F _ (5862.949) P < 0.001). It can be concluded that there is a linear correlation between the two cell adhesion rates. It can be concluded that the adhesion rate of the two cells at different time points is similar and there is a common increasing trend. (4) the proliferation ability of adipose mesenchymal stem cells is similar to that of bone marrow mesenchymal stem cells. After digestion, the second passage 5 cells experienced the incubation period of 24 hours 48 hours, entered the logarithmic growth phase of 6 to 9 days, and then transitioned to the plateau phase. (5) flow cytometry and immunocytochemistry showed that, Bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells expressed CD29 CD44, not CD34. Conclusion: (1) adipose mesenchymal stem cells similar to bone marrow mesenchymal stem cells can be extracted from fat of rats. (2) Surface markers of adipose mesenchymal stem cells and bone marrow mesenchymal stem cells can be obtained.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R329.2

【引證文獻(xiàn)】

相關(guān)期刊論文 前2條

1 任宇;吳海青;馬玉珍;倉(cāng)明;王瑞;劉東軍;;牛脂肪間充質(zhì)干細(xì)胞的分離、培養(yǎng)與鑒定[J];生物工程學(xué)報(bào);2010年12期

2 呂煒亮;廖毅;童庭輝;;雌激素誘導(dǎo)干細(xì)胞成骨研究新進(jìn)展[J];中國(guó)組織工程研究;2013年14期

本文編號(hào)：2156553