玻璃微流控芯片表面處理及其在DNA分析中的應(yīng)用
發(fā)布時(shí)間：2018-07-24 16:44
【摘要】：玻璃微流控芯片表面處理及其在DNA分析中的應(yīng)用 前言 微型全分析系統(tǒng)(Miniaturized Total Analysis Systems,μTAS)是當(dāng)前世界上最前沿的科技領(lǐng)域之一,其目的是通過(guò)化學(xué)分析設(shè)備的微型化和集成化,最大限度地把分析實(shí)驗(yàn)室的功能集成到便攜的分析設(shè)備中,甚至是方寸大小的芯片上,即“Lab on a chip”。微流控芯片是μTAS當(dāng)前最活躍的研究領(lǐng)域,以微通道網(wǎng)絡(luò)及眾多分析功能元件的集成化為其結(jié)構(gòu)特征,不僅使試樣和試劑的消耗顯著下降,而且具有高效、高速、高通量的分離分析能力。 生物醫(yī)學(xué)是當(dāng)前微流控芯片的主要應(yīng)用領(lǐng)域。芯片毛細(xì)管電泳是以微流控芯片上的微通道作為分離通道的分離分析技術(shù)。因微通道比表面積顯著增大,焦耳熱能很快向四周溢散,所以可施加平板凝膠電泳難以達(dá)到的高場(chǎng)強(qiáng),從而實(shí)現(xiàn)對(duì)樣品的快速、高效的分離檢測(cè)。 微流控芯片的微米級(jí)結(jié)構(gòu)顯著增大了微通道的比表面積,所以保持其內(nèi)表面的物理化學(xué)性質(zhì)的平衡、穩(wěn)定具有十分重要的意義。 玻璃具備優(yōu)良的光學(xué)性能,且易于進(jìn)行表面改性,是最廣泛使用的芯片基材之一。玻璃微流控芯片在進(jìn)行電泳分析時(shí),因微通道內(nèi)表面—SiOH基團(tuán)上的H~+解離,當(dāng)在微通道兩端施加外電場(chǎng)時(shí),通道內(nèi)整個(gè)緩沖溶液會(huì)流向陰極,形成電滲流(Electroosmotic Flow,EOF)。EOF不僅導(dǎo)致微通道表面對(duì)DNA樣品的吸附而且嚴(yán)重影響玻璃芯片對(duì)DNA樣品的分離效能,所以有效抑制EOF是玻璃芯片獲得高分離效率的前提。 對(duì)芯片微通道進(jìn)行表面改性處理是一種控制EOF的常用方法,可分為動(dòng)態(tài)涂層和永久表面改性。前者是最簡(jiǎn)單的表面改性方法,改性化合物可通過(guò)物理吸附結(jié)合在微通道表面實(shí)現(xiàn)動(dòng)態(tài)涂層。但因表面涂層不能長(zhǎng)期保持穩(wěn)定狀態(tài),其應(yīng)用受到限制。后者是控制EOF和減少樣品—表面吸附的最有效的方法。硅烷化試劑常用于永久改性處理中,其與微通道表面—SiOH基團(tuán)可共價(jià)鍵合,也可進(jìn)一步將線性聚合物交聯(lián)固定在通道表面形成
[Abstract]:Surface treatment of Glass Microfluidic Chip and its Application in DNA Analysis Analysis Systems, 渭 TAS is one of the most advanced fields of technology in the world. Its aim is to integrate the function of the analysis laboratory into the portable analysis equipment, even on the chip of square inch, that is, "Lab on a chip", through the miniaturization and integration of the chemical analysis equipment. Microfluidic chip is the most active research field in 渭 TAS at present. With the integration of microchannel network and many analytical functional components as its structural characteristics, the consumption of samples and reagents is significantly reduced, and the microfluidic chip has high efficiency and high speed. High throughput separation and analysis capability. Biomedicine is the main application field of microfluidic chip. Chip capillary electrophoresis (CE) is a separation analysis technique using microchannels on microfluidic chips as separation channels. Because the specific surface area of the microchannel is significantly increased and the Joule heat energy spills out rapidly, the high field strength which is difficult to achieve by plate gel electrophoresis can be applied to realize the rapid and efficient separation and detection of the samples. The micron structure of the microfluidic chip increases the specific surface area of the microchannel significantly, so it is of great significance to maintain the balance and stability of the physical and chemical properties of the inner surface of the microfluidic chip. Glass with excellent optical properties and easy surface modification is one of the most widely used chip substrates. When the glass microfluidic chip is analyzed by electrophoretic analysis, the whole buffer solution in the microchannel will flow to the cathode when the external electric field is applied to the two ends of the microchannel because of the dissociation of H ~ on the inner surface of the microchannel. The formation of electroosmotic flow (Electroosmotic flow EOF). EOF not only results in the adsorption of DNA on the surface of microchannels, but also seriously affects the separation efficiency of glass chips to DNA samples. Therefore, it is a prerequisite for glass chips to obtain high separation efficiency by effectively inhibiting EOF. Surface modification of microchannel is a common method to control EOF, which can be divided into dynamic coating and permanent surface modification. The former is the simplest surface modification method, and the modified compounds can be dynamically coated on the microchannel surface by physical adsorption. However, its application is limited because the surface coating can not be kept stable for a long time. The latter is the most effective method for controlling EOF and reducing sample-surface adsorption. Silanization reagents are often used in permanent modification treatment. They can be covalently bonded with the -SiOH group on the surface of the microchannel, and further, the linear polymer crosslinking can be fixed on the surface of the channel to form.
【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R346
[Abstract]:Surface treatment of Glass Microfluidic Chip and its Application in DNA Analysis Analysis Systems, 渭 TAS is one of the most advanced fields of technology in the world. Its aim is to integrate the function of the analysis laboratory into the portable analysis equipment, even on the chip of square inch, that is, "Lab on a chip", through the miniaturization and integration of the chemical analysis equipment. Microfluidic chip is the most active research field in 渭 TAS at present. With the integration of microchannel network and many analytical functional components as its structural characteristics, the consumption of samples and reagents is significantly reduced, and the microfluidic chip has high efficiency and high speed. High throughput separation and analysis capability. Biomedicine is the main application field of microfluidic chip. Chip capillary electrophoresis (CE) is a separation analysis technique using microchannels on microfluidic chips as separation channels. Because the specific surface area of the microchannel is significantly increased and the Joule heat energy spills out rapidly, the high field strength which is difficult to achieve by plate gel electrophoresis can be applied to realize the rapid and efficient separation and detection of the samples. The micron structure of the microfluidic chip increases the specific surface area of the microchannel significantly, so it is of great significance to maintain the balance and stability of the physical and chemical properties of the inner surface of the microfluidic chip. Glass with excellent optical properties and easy surface modification is one of the most widely used chip substrates. When the glass microfluidic chip is analyzed by electrophoretic analysis, the whole buffer solution in the microchannel will flow to the cathode when the external electric field is applied to the two ends of the microchannel because of the dissociation of H ~ on the inner surface of the microchannel. The formation of electroosmotic flow (Electroosmotic flow EOF). EOF not only results in the adsorption of DNA on the surface of microchannels, but also seriously affects the separation efficiency of glass chips to DNA samples. Therefore, it is a prerequisite for glass chips to obtain high separation efficiency by effectively inhibiting EOF. Surface modification of microchannel is a common method to control EOF, which can be divided into dynamic coating and permanent surface modification. The former is the simplest surface modification method, and the modified compounds can be dynamically coated on the microchannel surface by physical adsorption. However, its application is limited because the surface coating can not be kept stable for a long time. The latter is the most effective method for controlling EOF and reducing sample-surface adsorption. Silanization reagents are often used in permanent modification treatment. They can be covalently bonded with the -SiOH group on the surface of the microchannel, and further, the linear polymer crosslinking can be fixed on the surface of the channel to form.
【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R346
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