【摘要】：在過去的10余年時間里,人們對 “自身免疫”的認(rèn)識日趨深入。研究發(fā)現(xiàn),自身反應(yīng)性細(xì)胞不僅存在于自身免疫病患者,也存在于正常機體;應(yīng)用某些自身抗原免疫,通過增強或擴大某些自身免疫反應(yīng),對一些疾病(自身免疫病、損傷修復(fù))具有獨特的治療作用。這表明,“自身免疫”并不等同于“自身免疫病”,源自生理狀態(tài)下的某些自身免疫反應(yīng)可能對機體發(fā)揮著有益的作用。 以往研究表明:作為生理性自身免疫反應(yīng)的組成成分,自身反應(yīng)性B細(xì)胞存在于正常機體,并可分泌天然自身抗體(natural autoantibody,NAA),對維持機體自穩(wěn)、介導(dǎo)免疫耐受的形成有重要作用。然而,其介導(dǎo)生理性自身免疫反應(yīng)的機制尚不明確。在以往的研究基礎(chǔ)上,本課題以角蛋白反應(yīng)性B細(xì)胞及角蛋白反應(yīng)性NAA為研究切入點,對其介導(dǎo)免疫耐受的機制進(jìn)行探討。 目的:分析正常機體角蛋白反應(yīng)性B細(xì)胞的分布及比例,明確生理性自身免疫反應(yīng)的存在;闡明其可變區(qū)基因編碼特性,為進(jìn)一步探討角蛋白反應(yīng)性B細(xì)胞的作用奠定基礎(chǔ);研究角蛋白反應(yīng)性B細(xì)胞的抗原提呈作用,以及相應(yīng)NAA在凋亡細(xì)胞清除中作用,探討其介導(dǎo)免疫耐受的作用機制。 方法:實驗內(nèi)容共分5部分,實驗方法簡述如下: 1.檢測自身反應(yīng)性B細(xì)胞在正常機體的分布及比例 我們應(yīng)用FITC對5種蛋白抗原進(jìn)行了標(biāo)記,用流式細(xì)胞儀(flow cytometry,
[Abstract]:In the past 10 years, people's understanding of "autoimmune" is getting deeper and deeper. It has been found that its own reactive cells exist not only in the autoimmune disease patients but also in the normal body. Some autoantigens are used and some autoimmune reactions are enhanced or expanded, and some diseases (autoimmune diseases, injury repair) are enhanced or expanded. It has a unique therapeutic effect. This shows that "autoimmune" is not equal to "autoimmune disease", and some autoimmune reactions from physiological states may play a useful role in the body.
Previous studies have shown that as a component of the physiological autoimmune reaction, self reactive B cells exist in the normal body, and can secrete natural autoantibodies (natural autoantibody, NAA), which plays an important role in maintaining the body's self stability and mediating the formation of immune tolerance. However, the mechanism of its mediated autoimmune reaction is not yet clear. On the basis of previous studies, this topic is based on the study of keratin reactive B cells and keratin reactive NAA as a breakthrough point, and the mechanism of its mediated immune tolerance is discussed.
Objective: to analyze the distribution and proportion of keratin reactive B cells in normal body, to clarify the existence of physiological autoimmune reaction, to clarify the coding characteristics of its variable region gene, to lay the foundation for further exploration of the role of keratin reactive B cells, to study the antigen presenting effect of keratin reactive B cells and the corresponding NAA in apoptotic cells. To explore the mechanism of action mediated immune tolerance.
Methods: the experiment is divided into 5 parts. The experimental methods are summarized as follows:
1. detect the distribution and proportion of reactive B cells in the normal body.
We used FITC to label 5 kinds of protein antigens, using flow cytometry (flow cytometry).
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R392.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 張衍國,付萌,劉玉峰,高天文,潘蕾,王琳;EB病毒蛋白對抗角蛋白自身抗體產(chǎn)生的影響[J];中國皮膚性病學(xué)雜志;2003年06期

2 王剛,王琰,化冰,劉曉琳,喬媛媛;人源性天然抗體庫的構(gòu)建及初步鑒定[J];細(xì)胞與分子免疫學(xué)雜志;2002年01期

3 王剛!710032西安,王琰,化冰,喬媛媛,劉玉峰!710032西安;鏈替換技術(shù)提高抗角蛋白人Fab抗體的親和力[J];中華微生物學(xué)和免疫學(xué)雜志;2001年02期

4 李冰,李巍,劉玉峰,李承新,趙小東,高天文;抗角蛋白自身抗體對豚鼠銀屑病樣模型的影響[J];中國皮膚性病學(xué)雜志;2002年02期

，

本文編號：2141987