本文選題：腎陰虛 + SD大鼠��； 參考：《廣州中醫(yī)藥大學(xué)》2006年碩士論文

【摘要】：一、目的: 本課題的目的在于探索甲狀腺激素導(dǎo)致腎陰虛證動(dòng)物模型的規(guī)范造模方法,同時(shí)提供一個(gè)初步的腎陰虛證動(dòng)物模型評價(jià)標(biāo)準(zhǔn),為中醫(yī)證候動(dòng)物模型規(guī)范化研究提供參考。 二、方法: 1.理論研究:腎陰虛證動(dòng)物模型評價(jià)指標(biāo)選擇 1.1 通過查閱資料總結(jié)出臨床腎陰虛證診斷標(biāo)準(zhǔn)及客觀指標(biāo)研究成果,包括癥狀體征和客觀評價(jià)指標(biāo)。 1.2 根據(jù)臨床診斷標(biāo)準(zhǔn)和評價(jià)指標(biāo)初步擬定腎陰虛證動(dòng)物模型評價(jià)指標(biāo)。 2.實(shí)驗(yàn)研究(分以下四步進(jìn)行) 2.1 實(shí)驗(yàn)一:初步篩選甲狀腺素導(dǎo)致腎陰虛證大鼠模型的規(guī)范化造模方法。 本實(shí)驗(yàn)選擇SPF級雄性SD大鼠,根據(jù)文獻(xiàn)記載的最常用劑量設(shè)計(jì)出三個(gè)劑量:25mg/kgbw、12.5mg/kgbw、6.25mg/kgbw造模,分別在造模后第14天、21天、28天連續(xù)采血,觀察各劑量組動(dòng)物血清皮質(zhì)醇(Cort)、睪酮(T)、雌二醇(E_2)、甲狀腺素T_3和T_4、環(huán)磷酸腺苷(cAMP)、環(huán)磷酸鳥苷(cGMP)等各項(xiàng)指標(biāo)隨劑量、時(shí)間的變化,與空白對照組作對照,確定用藥劑量和給藥時(shí)間,篩選出較為規(guī)范的造模方法。 2.2 實(shí)驗(yàn)二:初步篩選甲狀腺素加利血平制作腎陰虛證大鼠模型的方法。 本實(shí)驗(yàn)選擇SPF級雄性SD大鼠,根據(jù)文獻(xiàn)記載的最常用劑量設(shè)計(jì)出三個(gè)劑量組:甲狀腺素37.5mg/kgbw、利血平0.25mg/kgbw;甲狀腺素75mg/kgbw、利血平0.5mg/kgbw;甲狀腺素150mg/kgbw、利血平1.0mg/kgbw造模,分別在造模后第6天、10天、14天連續(xù)采血,觀察各劑量組動(dòng)物血清皮質(zhì)醇(Cort)、睪酮(T)、雌二醇(E_2)、甲狀腺素T_3和T_4、環(huán)磷酸腺苷(cAMP)、環(huán)磷酸鳥苷(cGMP)等各項(xiàng)指標(biāo)隨劑量、時(shí)間的變化,與空白對照組作對照,確定用藥劑量和給藥時(shí)間,篩選出較為規(guī)范的造模方法。 2.3 實(shí)驗(yàn)三:甲狀腺激素造模法對雄性SD大鼠癥狀及其它指標(biāo)的影響 按實(shí)驗(yàn)一實(shí)驗(yàn)二篩選出的造模方法造模,在造模過程中對動(dòng)物進(jìn)行一般情況的觀察,并進(jìn)行耐力耐寒試驗(yàn)、抓取激惹試驗(yàn)、隔離攻擊試驗(yàn)、曠場試驗(yàn)、母鼠懷孕率等試驗(yàn),與空白對照組作對照。通過對癥狀的科學(xué)觀察,補(bǔ)充前面兩個(gè)實(shí)驗(yàn)客觀指標(biāo)檢測的不足,并進(jìn)一步完善腎陰虛證動(dòng)物模型癥狀診斷評價(jià)體系。 2.4 實(shí)驗(yàn)四:六味地黃湯的藥物反證 大鼠分為模型組、六味地黃湯組和空白對照組。模型組和六味地黃湯組均
[Abstract]:Objective: the purpose of this subject is to explore the standard method of establishing animal model of kidney yin deficiency syndrome caused by thyroid hormone. At the same time, it provides a preliminary evaluation standard for the animal model of kidney yin deficiency, and provides a reference for the standardization of animal model of TCM syndrome. Second, method: 1. Theoretical study: the evaluation index selection of animal model of kidney yin deficiency syndrome 1.1 through consulting data summarized clinical kidney yin deficiency diagnosis criteria and objective index research results. 1.2 according to the clinical diagnostic criteria and evaluation indicators, the evaluation index of animal model of kidney yin deficiency syndrome was preliminarily developed. 2. Experimental study (divided into four steps) 2.1 experiment 1: primary screening of the rat model of kidney yin deficiency induced by thyroxine. In this experiment, SPF male Sprague-Dawley rats were selected. According to the most commonly used doses recorded in the literature, three doses of 1: 25 mg / kg bww 12.5 mg / kg bww 6.25 mg / kg BW were designed. Blood samples were collected continuously on the 14th day, 21 days and 28 days after modeling, respectively. The changes of serum cortisol (Cort), testosterone (T), estradiol (E _ 2), thyroxine T3 and T _ 4, camp and cGMP were observed. The dosage and time of administration were determined, compared with the blank control group, and compared with the control group, the dosage and time of administration were determined, and the changes of serum cortisol (Cort), testosterone (T), estradiol (E _ S _ 2), thyroxine T _ 3 and T _ 4, cyclic adenosine phosphate (camp) and cyclic guanosine phosphate (cGMP) were determined. 2.2 experiment 2: the method of preliminary screening thyroxine plus reserpine to make the model of kidney yin deficiency syndrome in rats. Three dose groups were designed according to the most commonly reported doses: thyroxine 37.5 mg / kg bww, reserpine 0.25 mg / kg bww, thyroxine 75 mg / kg bww, reserpine 0.5 mg / kg bww, thyroxine 150 mg / kg bww, reserpine 1.0mg/kgbw. Serum cortisol (Cort), testosterone (T), estradiol (E2), thyroxine T3 and T4, cyclic adenosine phosphate (camp), cyclic guanosine monophosphate (cGMP) and so on were observed in serum of animals of each dose group, and the changes of serum cortisol (Cort), testosterone (T), estradiol (E2), thyroxine T3 and T4, cyclic adenosine phosphate (camp) and cyclic guanosine monophosphate (cGMP) were observed. As compared with the blank control group, the dosage and time of administration were determined. 2.3 experiment 3: effects of thyroid hormone modeling on symptoms and other indexes in male SD rats According to the model making method selected from experiment 1, experiment 2, In the course of modeling, the general situation of animals was observed, and the endurance cold tolerance test, capture irritation test, isolation attack test, open field test and pregnancy rate of female rats were carried out, which were compared with the blank control group. Through the scientific observation of the symptoms, the deficiencies of the previous two experimental objective indicators were supplemented. And further improve the animal model of kidney yin deficiency syndrome symptom diagnosis and evaluation system. 2.4 experiment 4: Liuwei Dihuang decoction drug reverse syndrome Rats were divided into model group, Liuwei Dihuang decoction group and blank control group. Model group and Liuwei Dihuang decoction group
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R-332

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