本文選題：人巨細(xì)胞病毒 + 感染�。� 參考：《山東大學(xué)》2006年碩士論文

【摘要】：第一部分 人巨細(xì)胞病毒感染對(duì)人臍靜脈內(nèi)皮細(xì)胞黏附分子和RAGE受體表達(dá)的影響 目的 觀察人巨細(xì)胞病毒(Human cytomegalovirus，HCMV)感染對(duì)人臍靜脈內(nèi)皮細(xì)胞(Human umbilical vein endothelial cells，HUVEC)的細(xì)胞間黏附分子-1(Intercellular adhesion molecule-1，ICAM-1)、血管細(xì)胞黏附分子-1(Vascular cell adhesion molecule-1，VCAM-1)及糖基化終產(chǎn)物受體(the Receptor for advanced glycation end products，RAGE)時(shí)序性表達(dá)的影響，研究HCMV感染致動(dòng)脈粥樣硬化(Atherosclerosis，AS)的作用機(jī)制。 方法 用HCMV感染HUVEC，采用RT-PCR方法檢測(cè)不同感染時(shí)段細(xì)胞ICAM-1、VCAM-1及RAGE的mRNA表達(dá)。 結(jié)果 1．HCMV感染對(duì)HUVEC ICAM-1mRNA表達(dá)的影響 HCMV感染HUVEC細(xì)胞后，0h時(shí)ICAM-1mRNA有基礎(chǔ)水平的表達(dá)，4h開始升高，8h表達(dá)量達(dá)高峰，12h開始回落，48h表達(dá)量仍維持在較高的水平，各時(shí)段與0h比較，均有統(tǒng)計(jì)學(xué)意義(P＜0.01)。 2．HCMV感染對(duì)HUVEC VCAM-1mRNA表達(dá)的影響 HCMV感染HUVEC細(xì)胞后，0h時(shí)VCAM-1mRNA有基礎(chǔ)水平的表達(dá)，4h開始升高，8h時(shí)達(dá)高峰，12h開始回落，24h回落更明顯，，與0h比較，均有統(tǒng)計(jì)學(xué)意義(P＜0.01)，48h接近0h表達(dá)水平(P＞0.05)。
[Abstract]:Effect of human cytomegalovirus infection on the expression of adhesion molecules and rage receptors in human umbilical vein endothelial cells Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (Vascular) induced by human cytomegalovirus (HCMV) infection in human umbilical vein endothelial cells were observed. The effects of cell adhesion molecule-1 (VCAM-1) and the receptor for advanced glycation end products rage on the temporal expression of VCAM-1, To study the mechanism of atherosclerosis induced by HCMV infection. Methods HUVECs were infected with HCMV, and the mRNA expression of ICAM-1VCAM-1 and rage were detected by RT-PCR. The effect of HCMV infection on the expression of ICAM-1 mRNA in HUVEC The expression of ICAM-1 mRNA at 0 h after HUVEC increased at 4 h and reached the peak at 12 h and decreased at 48 h. The expression of ICAM-1 mRNA remained at a higher level at 48 h. Compared with 0h, 2. The effect of HCMV infection on the expression of VCAM-1 mRNA in HUVEC After HCMV was infected with HUVEC cells, the expression of VCAM-1 mRNA at 0 h after HCMV infection began to increase at 4 h and reached the peak at 12 h, and decreased at 12 h and decreased significantly at 24 h after HCMV infection, and the expression of VCAM-1 mRNA increased at 4 h after HCMV infection. Compared with 0 h, there was significant difference (P < 0.01) between 0 h and 48 h (P > 0.05).
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R373

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 程敏,聞良珍,凌霞珍,趙捷;人巨細(xì)胞病毒感染對(duì)臍靜脈內(nèi)皮細(xì)胞粘附分子和核轉(zhuǎn)錄因子表達(dá)的影響[J];中華微生物學(xué)和免疫學(xué)雜志;2003年07期

2 鄭超,文格波,曹仁賢,劉江華,黃雌友;糖基化終末產(chǎn)物對(duì)人臍靜脈內(nèi)皮細(xì)胞凋亡及其受體表達(dá)的影響[J];中華內(nèi)分泌代謝雜志;2003年02期

本文編號(hào)：2111582