本文選題：兒茶酚胺 + 泡沫細(xì)胞��； 參考：《四川大學(xué)》2005年博士論文

【摘要】：第一部分 心血管系統(tǒng)是心理應(yīng)激的靶目標(biāo),伴有運(yùn)動(dòng)誘導(dǎo)的心肌缺血,血壓升高,心率增加,心律不齊,動(dòng)脈粥樣硬化的發(fā)展,以及死亡。最近的資料指出在下丘腦,垂體腺,腎上腺髓質(zhì)和交感神經(jīng)末端之間存在相互作用如同對(duì)應(yīng)激的神經(jīng)內(nèi)分泌反應(yīng)。心理應(yīng)激導(dǎo)致細(xì)胞機(jī)能障礙的機(jī)制了解甚少,在動(dòng)脈粥樣硬化中表達(dá)上調(diào)的多種基因,也可被心理應(yīng)激誘導(dǎo),精神應(yīng)激導(dǎo)致細(xì)胞功能失調(diào)并最終導(dǎo)致心血管疾病的更多的下游信號(hào)仍然大部分都未知。 目的:觀察兒茶酚胺類激素對(duì)THP-1巨噬細(xì)胞源性泡沫細(xì)胞泡沫化和細(xì)胞內(nèi)膽固醇流出的影響。同時(shí)觀察兒茶酚胺類激素對(duì)THP-1巨噬細(xì)胞源性泡沫細(xì)胞TGF-β1、CD36、SR-AⅠ、ABCA1、Caveolin-1基因表達(dá)的影響。初步了解兒茶酚胺類激素影響THP-1巨噬細(xì)胞源性泡沫細(xì)胞功能的細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)途徑。 方法:運(yùn)用高效液相色譜檢測(cè)細(xì)胞內(nèi)總膽固醇和游離膽固醇的含量,液體閃爍計(jì)數(shù)器檢測(cè)細(xì)胞內(nèi)膽固醇流出,逆轉(zhuǎn)錄-多聚酶鏈?zhǔn)椒磻?yīng)檢測(cè)TGF-β1、CD36、SR-AⅠ、ABCA1、Caveolin-1 mRNA的表達(dá),酶聯(lián)免疫吸附試驗(yàn)檢測(cè)TGF-β1、Western印跡檢測(cè)SR-AⅠ和ABCA1蛋白的表達(dá)。 結(jié)果:不同濃度的去甲腎上腺素(10 nmol/L～10μmol/L)作用THP-1巨噬細(xì)胞源性泡沫細(xì)胞24小時(shí)。結(jié)果顯示,1μmol/L和10μmol/L去甲腎上腺素處理后,細(xì)胞內(nèi)總膽固醇、游離膽固醇和膽固醇酯明顯增加,呈劑量和時(shí)間依賴性(p0.05)。而apoA-Ⅰ介導(dǎo)的細(xì)胞內(nèi)膽固醇流出呈時(shí)間依賴性減少(p0.05)。100nmol/L及1μmol/L的腎上腺素作用THP-1巨噬細(xì)胞源性泡沫細(xì)胞后,其TGF-β1、ABCA1 mRNA水平下降(p0.01;p0.05),SR-AⅠmRNA水平升高(p0.05)。100 nmol/L,1μmol/L及10μmol/L的去甲腎上腺素作用THP-1巨噬細(xì)胞源性泡沫細(xì)胞后,其TGF-β1、ABCA1 mRNA和蛋白水平下降(p0.05);1μmol/L及10μmol/L的去甲腎上腺素則升高SR-AⅠmRNA和蛋白的水平(p0.05)。而腎上腺素和去甲腎上腺素作用細(xì)胞后對(duì)CD36和Caveolin-1基因表達(dá)無影響(p0.05)。為了解去甲腎上腺素影響TGF-β1、SR-AⅠ
[Abstract]:The first part of the cardiovascular system is the target of psychological stress, accompanied by exercise induced myocardial ischemia, elevated blood pressure, increased heart rate, irregular rhythm, The development of atherosclerosis, as well as death. Recent data suggest that there are interactions between hypothalamus, pituitary gland, adrenal medulla and sympathetic nerve terminals as well as neuroendocrine responses to stress. Little is known about the mechanisms of psychological stress leading to cellular dysfunction, and many genes that are up-regulated in atherosclerosis can also be induced by psychological stress. Mental stress causes cell dysfunction and ultimately leads to more downstream signals from cardiovascular disease that remain largely unknown. Aim: to observe the effect of catecholamines on foaming and cholesterol efflux of THP-1 macrophage derived foam cells. Meanwhile, the effects of catecholamines on the expression of Caveolin-1 gene in THP-1 macrophage-derived foam cells were observed. To understand the cellular signal transduction pathway of catecholamines affecting the function of THP-1 macrophage derived foam cells. Methods: the contents of total cholesterol and free cholesterol in cells were detected by high performance liquid chromatography, cholesterol efflux was detected by liquid scintillation counter, and the expression of TGF- 尾 1 CD36A 鈪，

本文編號(hào)：2107889