本文選題：兒茶酚胺 + 泡沫細胞�。� 參考：《四川大學》2005年博士論文

【摘要】：第一部分 心血管系統(tǒng)是心理應激的靶目標,伴有運動誘導的心肌缺血,血壓升高,心率增加,心律不齊,動脈粥樣硬化的發(fā)展,以及死亡。最近的資料指出在下丘腦,垂體腺,腎上腺髓質(zhì)和交感神經(jīng)末端之間存在相互作用如同對應激的神經(jīng)內(nèi)分泌反應。心理應激導致細胞機能障礙的機制了解甚少,在動脈粥樣硬化中表達上調(diào)的多種基因,也可被心理應激誘導,精神應激導致細胞功能失調(diào)并最終導致心血管疾病的更多的下游信號仍然大部分都未知。 目的:觀察兒茶酚胺類激素對THP-1巨噬細胞源性泡沫細胞泡沫化和細胞內(nèi)膽固醇流出的影響。同時觀察兒茶酚胺類激素對THP-1巨噬細胞源性泡沫細胞TGF-β1、CD36、SR-AⅠ、ABCA1、Caveolin-1基因表達的影響。初步了解兒茶酚胺類激素影響THP-1巨噬細胞源性泡沫細胞功能的細胞信號轉(zhuǎn)導途徑。 方法:運用高效液相色譜檢測細胞內(nèi)總膽固醇和游離膽固醇的含量,液體閃爍計數(shù)器檢測細胞內(nèi)膽固醇流出,逆轉(zhuǎn)錄-多聚酶鏈式反應檢測TGF-β1、CD36、SR-AⅠ、ABCA1、Caveolin-1 mRNA的表達,酶聯(lián)免疫吸附試驗檢測TGF-β1、Western印跡檢測SR-AⅠ和ABCA1蛋白的表達。 結(jié)果:不同濃度的去甲腎上腺素(10 nmol/L～10μmol/L)作用THP-1巨噬細胞源性泡沫細胞24小時。結(jié)果顯示,1μmol/L和10μmol/L去甲腎上腺素處理后,細胞內(nèi)總膽固醇、游離膽固醇和膽固醇酯明顯增加,呈劑量和時間依賴性(p0.05)。而apoA-Ⅰ介導的細胞內(nèi)膽固醇流出呈時間依賴性減少(p0.05)。100nmol/L及1μmol/L的腎上腺素作用THP-1巨噬細胞源性泡沫細胞后,其TGF-β1、ABCA1 mRNA水平下降(p0.01;p0.05),SR-AⅠmRNA水平升高(p0.05)。100 nmol/L,1μmol/L及10μmol/L的去甲腎上腺素作用THP-1巨噬細胞源性泡沫細胞后,其TGF-β1、ABCA1 mRNA和蛋白水平下降(p0.05);1μmol/L及10μmol/L的去甲腎上腺素則升高SR-AⅠmRNA和蛋白的水平(p0.05)。而腎上腺素和去甲腎上腺素作用細胞后對CD36和Caveolin-1基因表達無影響(p0.05)。為了解去甲腎上腺素影響TGF-β1、SR-AⅠ
[Abstract]:The first part of the cardiovascular system is the target of psychological stress, accompanied by exercise induced myocardial ischemia, elevated blood pressure, increased heart rate, irregular rhythm, The development of atherosclerosis, as well as death. Recent data suggest that there are interactions between hypothalamus, pituitary gland, adrenal medulla and sympathetic nerve terminals as well as neuroendocrine responses to stress. Little is known about the mechanisms of psychological stress leading to cellular dysfunction, and many genes that are up-regulated in atherosclerosis can also be induced by psychological stress. Mental stress causes cell dysfunction and ultimately leads to more downstream signals from cardiovascular disease that remain largely unknown. Aim: to observe the effect of catecholamines on foaming and cholesterol efflux of THP-1 macrophage derived foam cells. Meanwhile, the effects of catecholamines on the expression of Caveolin-1 gene in THP-1 macrophage-derived foam cells were observed. To understand the cellular signal transduction pathway of catecholamines affecting the function of THP-1 macrophage derived foam cells. Methods: the contents of total cholesterol and free cholesterol in cells were detected by high performance liquid chromatography, cholesterol efflux was detected by liquid scintillation counter, and the expression of TGF- 尾 1 CD36A 鈪，

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